SNU-16 cell

SKU:BHC11101451
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Overview
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SNU-16 cell is a cell line derived from East Asian (Female). It is commonly used as an in vitro model for 1 research. Growth characteristics: Suspension, multicellular aggregates, Epithelial. Supplied as cryopreserved cells with accompanying batch CoA and quality-control documentation.

Species Human
Disease model Adenocarcinoma
Morphology Epithelial
Growth Properties Suspension, multicellular aggregates
Tissue Stomach
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Catalog no. Size
305273 1 cryovial
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This cell line is available in the U.S. For non-profit users, please sign and submit the Non-Profit Supply Agreement to orders@biohippo.com before placing an order. For commercial users, please complete the CLEAR Form before ordering, as additional usage fees may apply based on the intended use. For further details, please contact orders@biohippo.com. Products ship after the required agreement is completed; typical delivery is 2–3 business days. Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10^6 cells for adherent lines or 5 × 10^6 cells for suspension lines (refer to the batch CoA for details).

Field Specification
Species Human
The SNU-16 cell line is derived from a poorly differentiated gastric carcinoma of a human adult. This cell line is extensively used in gastric cancer research, offering a model to study the molecular and cellular mechanisms involved in the development and progression of gastric adenocarcinoma. SNU-16 cells are particularly valuable for investigating genetic alterations, signal transduction pathways, and the tumor microenvironment associated with this aggressive form of stomach cancer. SNU-16 cells exhibit an epithelial morphology and are characterized by the expression of gastric carcinoma markers, including carcinoembryonic antigen (CEA) and various cytokeratins. They are known to possess amplification of the c-MET gene and overexpression of the MET receptor, which plays a significant role in cell growth, survival, and metastasis. Researchers use SNU-16 cells to explore the role of the MET signaling pathway in gastric cancer and to evaluate the efficacy of MET inhibitors and other targeted therapies. Additionally, SNU-16 cells are utilized in drug resistance studies, high-throughput screening assays, and preclinical testing of new chemotherapeutic agents. The relevance of the SNU-16 cell line in gastric cancer research underscores its importance in advancing our understanding of the disease and developing more effective treatment strategies for gastric cancer patients.

SKU:BHC11101451

  • Surface antigens: Blood Type A, Rh +, carcinoembryonic antigen (CEA) and TAG 72
  • Oncogenes: myc +, erb-B2 +
  • Tumorigenic: Yes, in semisolid medium
  • Mutational profile: Mutation: MSH6, p.Lys1358fs*2 (c.4065_4066insTTGA), heterozygous; Mutation: TP53, p.Tyr205Phe (c.614A>T), homozygous
  • cultureMedium: RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
  • supplements: Supplement the medium with 10% FBS, 25 mM HEPES
  • subculturing: Suspension cells: Remove cells from substrate by pipetting with fresh medium. To obtain single cells, pass the suspension several times through a 22 gauge needle and dispense into new flasks.
  • fluidRenewal: 2 times per week
  • freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.

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