AnRE Reporter Lentivirus

Background

The androgen response element (AnRE) is a DNA sequence that mediates transcription driven by the androgen receptor (AR), a ligand-activated nuclear receptor. Upon binding androgens such as testosterone or dihydrotestosterone, AR translocates to the nucleus, dimerizes, and binds androgen response elements to regulate genes controlling male sexual differentiation, muscle and bone physiology, and prostate function. AR signaling is a central driver of prostate cancer, where it remains a key therapeutic target, and is also studied in endocrinology and steroid hormone biology. AnRE-driven reporters give a quantitative readout of androgen receptor transcriptional activity.

Product Description & Applications

The AnRE Reporter Lentivirus is a transcription factor reporter system that places a reporter gene under the control of tandem androgen response elements coupled to a minimal promoter, giving a sensitive fluorescent or luminescent readout of androgen receptor activation. Reporter options include EGFP, GFP, mCherry, RFP, firefly luciferase, and Renilla luciferase, with optional blasticidin or puromycin selection for stable polyclonal cell line generation. Particles are purified by PEG precipitation and sucrose gradient centrifugation and efficiently transduce difficult-to-transfect cells, including primary and thawed cells.

Applications include monitoring AR signaling pathway activity, characterizing androgens and anti-androgens, and screening compounds that modulate androgen receptor function in endocrinology and cancer research.

About This Product

This reporter lentivirus places a EGFP, Firefly Luc, GFP, Luc, mCherry, Renilla Luc, RFP reporter gene under the control of tandem consensus response elements specific for the Androgen receptor (AR) signaling pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.

Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.