| Field | Specification |
|---|---|
| Product Type | |
| Promoter | |
| Reporter | |
| Selection Marker | Puromycin, Blasticidin |
| Shipping | |
| Species |
Background
ATF4 (activating transcription factor 4) is a basic leucine zipper transcription factor and a master effector of the integrated stress response. In response to amino acid deprivation, endoplasmic reticulum stress, oxidative stress, and other insults, stress-sensing kinases phosphorylate the translation initiation factor eIF2-alpha, which selectively promotes ATF4 translation. ATF4 then binds C/EBP-ATF response elements (CARE) and induces genes for amino acid transport and synthesis, including aminoacyl-tRNA synthetases, as well as genes governing redox balance, autophagy, and cell fate. Through these activities ATF4 coordinates adaptation to stress and is implicated in cancer metabolism, neurodegeneration, and metabolic disease.
Product Description & Applications
The ATF4 Reporter Lentivirus places a reporter gene under the control of tandem C/EBP-ATF response elements (CARE), providing a sensitive readout of ATF4 transcriptional activity and the amino acid stress response in human and mouse cells. Reporter options include fluorescent (GFP, RFP, EGFP, mCherry) and luminescent (firefly and Renilla luciferase) formats, with puromycin or blasticidin selection for stable cell line establishment. Signal can be measured by fluorescence microscopy, flow cytometry, or luminometry.
The particles are purified by PEG precipitation and sucrose gradient centrifugation and efficiently transduce difficult-to-transfect cells, including primary and cryopreserved cultures. Applications include monitoring the integrated stress response and screening compounds that modulate ATF4 signaling.
About This Product
This reporter lentivirus places a EGFP, Firefly Luc, GFP, Luc, mCherry, Renilla Luc, RFP reporter gene under the control of tandem consensus response elements specific for the ATF4/Amino acid response transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Puromycin, Blasticidin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
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