| Field | Specification |
|---|---|
| Accession Number | |
| Product Type | |
| Reporter | |
| Selection Marker | Puromycin, Blasticidin, N/A |
| Shipping | |
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Background
Acyl-CoA synthetase long-chain family member 4 (ACSL4) is an enzyme that activates long-chain polyunsaturated fatty acids, particularly arachidonic and adrenic acid, by converting them to their acyl-CoA derivatives for incorporation into membrane phospholipids. Through this preference, ACSL4 shapes the cellular lipidome and is a key determinant of sensitivity to ferroptosis, an iron-dependent form of regulated cell death driven by lipid peroxidation. ACSL4 also participates in lipid metabolism, eicosanoid signaling, and steroidogenesis. Its expression influences tumor cell survival, drug response, and tissue injury, making ACSL4 an important target in ferroptosis, cancer metabolism, and cell death research.
Product Description & Applications
The h/m ACSL4 shRNA Lentivirus delivers validated short hairpin RNA targeting human and mouse ACSL4 from a third-generation, self-inactivating lentiviral backbone. shRNA expression is driven by a U6 promoter, with a co-expressed fluorescent reporter (GFP or RFP) and antibiotic selection marker. VSV-G pseudotyping supports broad tropism across primary, suspension, and cryopreserved cells, and each shRNA is validated for at least 70% ACSL4 knockdown by a fluorescence-based method.
High-titer particles are ultra-purified by PEG precipitation and sucrose gradient centrifugation. A shRNA set option provides a mix of two independent validated shRNAs plus a scrambled control. Applications include loss-of-function studies of ferroptosis, lipid metabolism, and cancer cell survival.
About This Product
This validated shRNA lentivirus targeting ACSL4 (NCBI Accession: NM_004458.3) delivers a 19–20 bp shRNA from a third-generation, self-inactivating lentiviral backbone. Expression is driven from a U6 Pol III promoter, with a constitutively expressed fluorescent reporter (GFP, GFP/Luc, RFP, RFP/Luc) and antibiotic selection marker (Puromycin, Blasticidin) co-expressed from the same vector. VSV-G pseudotyping enables broad cell tropism, including primary, suspension, and cryopreserved cell types.
Knockdown is validated using a proprietary bicistronic fluorescence assay in which the target mRNA is co-expressed fused to RFP alongside the shRNA-GFP construct. At least 70% reduction in RFP signal in GFP-positive cells confirms on-target activity — a more direct functional readout than transcript-level qPCR. Polyclonal stable lines can be generated by antibiotic selection within 10 days, preserving parental cell heterogeneity compared to single-clone CRISPR approaches.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
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