| Field | Specification |
|---|---|
| Accession Number | |
| Product Type | |
| Reporter | |
| Selection Marker | Blasticidin, N/A, Puromycin |
| Shipping | |
| Species |
Background
Neuropilin 2 (NRP2) is a single-pass transmembrane glycoprotein that functions as a co-receptor for class 3 semaphorins and for members of the vascular endothelial growth factor (VEGF) family. By modulating semaphorin and VEGF signaling, NRP2 regulates axon guidance, lymphatic and vascular development, and cell migration. NRP2 is also expressed on immune cells and tumor cells, where it influences cell adhesion, motility, and survival. Aberrant NRP2 expression contributes to tumor progression, lymphangiogenesis, and metastasis in several cancers, making it an actively investigated target in cancer biology, angiogenesis research, and neurodevelopmental studies.
Product Description & Applications
The h/m NRP2 shRNA Lentivirus delivers a validated short hairpin RNA that achieves at least 70% knockdown of human and mouse Neuropilin 2. The shRNA is expressed from a U6 promoter in a third-generation, self-inactivating lentiviral backbone, with a co-expressed fluorescent reporter (GFP or RFP, optionally with luciferase) and antibiotic selection marker for stable cell line generation. Knockdown is confirmed using a rapid fluorescence-based assay. The shRNA lentivirus set provides particles from a mix of two independent validated shRNAs plus a matched scrambled control. Applications include loss-of-function studies of NRP2 in tumor progression, lymphangiogenesis, cell migration, and semaphorin/VEGF signaling. Particles are ultra-purified and concentrated to high titer by PEG precipitation and sucrose gradient centrifugation, transducing difficult-to-transfect cells, including primary and thawed cultures.
About This Product
This validated shRNA lentivirus targeting NRP2 (NCBI Accession: NM_201266.2) delivers a 19–20 bp shRNA from a third-generation, self-inactivating lentiviral backbone. Expression is driven from a U6 Pol III promoter, with a constitutively expressed fluorescent reporter (GFP, GFP/Luc, RFP, RFP/Luc) and antibiotic selection marker (Blasticidin, Puromycin) co-expressed from the same vector. VSV-G pseudotyping enables broad cell tropism, including primary, suspension, and cryopreserved cell types.
Knockdown is validated using a proprietary bicistronic fluorescence assay in which the target mRNA is co-expressed fused to RFP alongside the shRNA-GFP construct. At least 70% reduction in RFP signal in GFP-positive cells confirms on-target activity — a more direct functional readout than transcript-level qPCR. Polyclonal stable lines can be generated by antibiotic selection within 10 days, preserving parental cell heterogeneity compared to single-clone CRISPR approaches.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
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