| Field | Specification |
|---|---|
| Accession Number | |
| Product Type | |
| Reporter | |
| Selection Marker | Puromycin |
| Shipping | |
| Species |
Background
miR-128a-3p is the mature microRNA derived from the MIR128-1 stem-loop and is conserved between human and mouse. Like other microRNAs, it is processed from a primary transcript by the Drosha/DGCR8 and Dicer enzymes and loaded into the RNA-induced silencing complex, where it base-pairs with target mRNAs to repress their translation or promote their degradation. miR-128a-3p is enriched in neural tissue and regulates genes involved in cell proliferation, differentiation, and signal transduction. Through this network of targets it has been studied as a modulator of neuronal development and as a candidate tumor-suppressive microRNA in several cancer types, making it a useful tool for gene-regulation and oncology research.
Product Description & Applications
This miRNA lentivirus delivers the complete human miR-128a stem-loop, which is processed in transduced cells to yield the mature miR-128a-3p sequence common to human and mouse. The stem-loop is driven by a U6 promoter, while a PGK promoter drives a GFP reporter and a puromycin selection marker separated by a self-cleaving peptide, so transduced cells can be identified by fluorescence and enriched by antibiotic selection or FACS.
The particles are ultra-purified and concentrated by PEG precipitation and sucrose gradient centrifugation, supporting in vitro and in vivo use and transduction of difficult-to-transfect cells such as primary and thawed cultures. Polyclonal stable cell lines can be established within about 10 days for long-term studies of miR-128a function, target repression, and microRNA biology while preserving parental cell properties.
About This Product
This lentivirus expresses the full pre-miRNA stem-loop sequence of MIR128-1 under a Pol II promoter (CMV or EF1α), enabling endogenous Drosha/DGCR8 and Dicer processing to produce authentic mature 3P (and 5p) strands loaded into RISC. This produces physiologically relevant miRNA activity that faithfully replicates the endogenous biogenesis pathway, unlike synthetic miRNA mimics that bypass nuclear processing and can exhibit RISC overloading artifacts.
Co-expression of a fluorescent reporter (GFP) and selection marker (Puromycin) allows stable transductant identification by fluorescence and antibiotic enrichment. Stable miRNA overexpression enables long-term target repression studies, in vivo tumor models, and exosomal miRNA loading experiments that are not achievable with transient miRNA mimics.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
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