| Field | Specification |
|---|---|
| Accession Number | |
| Product Type | |
| Reporter | |
| Selection Marker | Puromycin |
| Shipping | |
| Species |
Background
miR-375 is a conserved microRNA that post-transcriptionally regulates gene expression by guiding the RNA-induced silencing complex to complementary sequences in target mRNAs, leading to translational repression or transcript destabilization. miR-375 is highly enriched in pancreatic islets, where it influences insulin secretion and beta-cell function, and it is also expressed in epithelial and neuroendocrine tissues. It frequently behaves as a tumor-suppressive microRNA, with altered expression reported across several carcinomas. Through these activities, miR-375 contributes to endocrine regulation and cancer biology and is widely studied as a functional and biomarker microRNA.
Product Description & Applications
The miR-375-3p-GFP-Puro Lentivirus delivers stable overexpression of human and mouse miR-375. The vector carries the entire miRNA stem-loop, allowing endogenous Drosha and Dicer processing to generate the authentic mature miR-375-3p strand for loading into the RNA-induced silencing complex. The stem-loop is driven by a U6 promoter, while a PGK promoter drives a GFP fluorescent reporter and a puromycin selection marker separated by a self-cleaving peptide. Transduced cells are readily identified and enriched by GFP fluorescence or puromycin selection, with polyclonal stable lines established within about ten days while preserving parental cell properties. It is used for long-term target-repression studies, tumor models, and other functional microRNA experiments. Supplied as ultra-purified, high-titer lentiviral particles suitable for in vitro and in vivo use and for primary or thawed cells.
About This Product
This lentivirus expresses the full pre-miRNA stem-loop sequence of MIR375 under a Pol II promoter (CMV or EF1α), enabling endogenous Drosha/DGCR8 and Dicer processing to produce authentic mature 3P (and 5p) strands loaded into RISC. This produces physiologically relevant miRNA activity that faithfully replicates the endogenous biogenesis pathway, unlike synthetic miRNA mimics that bypass nuclear processing and can exhibit RISC overloading artifacts.
Co-expression of a fluorescent reporter (GFP) and selection marker (Puromycin) allows stable transductant identification by fluorescence and antibiotic enrichment. Stable miRNA overexpression enables long-term target repression studies, in vivo tumor models, and exosomal miRNA loading experiments that are not achievable with transient miRNA mimics.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
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