| Field | Specification |
|---|---|
| Product Type | |
| Promoter | |
| Reporter | |
| Selection Marker | Blasticidin, Puromycin |
| Shipping | |
| Species |
Background
The retinoid X receptor (RXR) is a nuclear hormone receptor that acts as a master regulator of transcription by binding specific DNA sequences in the promoters of target genes. RXR recognizes direct-repeat elements such as DR1, and its activity depends on dimerization state: RXR/RXR homodimers respond to 9-cis retinoic acid, whereas RXR/RAR heterodimers can repress transcription through the same element. As an obligate partner for many other nuclear receptors, RXR integrates retinoid, lipid, and hormonal signals. Retinoic acid signaling through RXR governs cell differentiation, proliferation, and development, and dysregulation of this pathway is implicated in cancer and metabolic disease.
Product Description & Applications
The RXRE/DR1 Reporter Lentivirus is a lentiviral reporter system that provides a sensitive fluorescent (GFP or RFP) or luminescent (firefly luciferase) readout of RXR transcriptional activity in mammalian cells. The reporter contains tandem repeats of the consensus DR1 DNA-binding element, which is activated by RXR/RXR homodimers and suppressed by RXR/RAR heterodimers in response to 9-cis retinoic acid. It is used to study retinoic acid signaling and to quantify RXR-mediated transcription. All versions include a drug-selection marker (puromycin or blasticidin) for straightforward establishment of stable cell lines. Supplied as lentiviral particles purified by PEG precipitation and sucrose gradient centrifugation, the product is well suited to difficult-to-transfect cells, including primary and thawed cells.
About This Product
This reporter lentivirus places a EGFP, Firefly Luc, GFP, Luc, mCherry, Renilla Luc, RFP reporter gene under the control of tandem consensus response elements specific for the RXR signaling pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
Metabolic stress sensing by epithelial RXRα links westernization of diet with Crohn's disease.
Meyer M, Grabherr F, Plattner C, Hadjihannas MV, et al.
Cell Metabolism, 2026. DOI: 10.1016/j.cmet.2025.11.008
Product(s) used: LTV-0047
Usage: RXRE/DR1 Reporter Lentivirus (LTV-0047-3S) stably transduced into MODE-K intestinal epithelial cells; firefly luciferase readout used to monitor RXRα transcriptional activity under lipid metabolic stress and dietary fat in a Crohn's disease model.
View article →Research budgets are tight — we get it. That's why we've put together a fresh round of exclusive promotions designed to help you stock up on the reagents, kits, and consumables your lab depends on, without stretching your budget.
🔬 What's on offer right now:
10% Off Pre-Designed siRNA Sets
20% Off Transmembrane Proteins
50% Off Lab Consumables + Free Shipping
$99 Pipette Filler Promotion Package
BlasTaq 2X qPCR MasterMix - 50% OFF Limited Time Offer
DENARASE® Endonuclease — 10% Off One Order
10% OFF CELL LINES-Limited-Time Offer
