| Field | Specification |
|---|---|
| Accession Number | |
| Product Type | |
| Promoter | |
| Reporter | |
| Selection Marker | Blasticidin, N/A, Puromycin |
| Shipping | |
| Species |
Background
The serum response element (SRE) is a promoter motif that mediates transcriptional responses to growth factor and mitogen signaling, classically studied in the c-Fos promoter. The SRE is bound by serum response factor (SRF) together with ternary complex factors such as ELK-1. Growth factor stimulation activates the MAPK/ERK cascade, leading to ERK1/2 (MAPK3/MAPK1)-mediated phosphorylation of ELK-1, which together with SRF drives rapid induction of immediate-early genes. This pathway controls cell proliferation, differentiation, and survival, and its dysregulation is implicated in cancer and other proliferative disorders, making SRE-driven reporters valuable for monitoring MAPK/ERK and growth factor signaling.
Product Description & Applications
The SRE Reporter Lentivirus places a reporter gene under the control of tandem c-Fos serum response elements, providing a sensitive readout of MAPK/ERK and growth factor signaling through serum response factor and its cofactors, including ELK-1. Reporter options include fluorescent (GFP, RFP), luminescent (firefly and Renilla luciferase), and combined GFP/Luc and RFP/Luc formats, with blasticidin or puromycin selection for stable reporter cell line establishment.
The particles are purified by PEG precipitation and sucrose gradient centrifugation and efficiently transduce difficult-to-transfect cells, including primary and cryopreserved cultures. Applications include monitoring MAPK/ERK pathway activation, studying mitogen and growth factor responses, and screening modulators of this signaling axis.
About This Product
This reporter lentivirus places a Firefly Luc, GFP, GFP/Luc, Luc, Renilla Luc, RFP, RFP/Luc reporter gene under the control of tandem consensus response elements specific for the MAPK/ERK pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
A neuroimmune pathway drives bacterial infection.
Wang N, Liu J, Wu R, Chen F, et al.
Science Advances, 2025. DOI: 10.1126/sciadv.adr2226
Product(s) used: LTV-0028
Usage: SRE/MAPK Reporter Lentivirus (LTV-0028, LipExoGen) transduced into bone marrow-derived macrophages; fluorescent SRE reporter quantified MAPK/ERK activation by LPS and dopamine during bacterial infection neuroimmune response.
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