| Field | Specification |
|---|---|
| Product Type | |
| Promoter | |
| Reporter | |
| Selection Marker | Blasticidin, Puromycin |
| Shipping | |
| Species |
Background
The thyroid hormone response element arranged as a direct repeat spaced by four nucleotides (T3RE/DR4) is the DNA binding site recognized by thyroid hormone receptors. These nuclear receptors heterodimerize with the retinoid X receptor and, upon binding the active thyroid hormone triiodothyronine (T3), switch from transcriptional repression to activation of target genes. Thyroid hormone signaling regulates basal metabolic rate, growth, cardiac function, and neural development. In this system thyroid stimulating hormone receptor (TSHR) signaling drives T3-dependent transcription through the DR4 element, providing a readout of thyroid pathway activity. Dysregulated thyroid hormone signaling underlies hypothyroidism, hyperthyroidism, and metabolic disease, making this pathway important in endocrinology research.
Product Description & Applications
The T3RE/DR4 Reporter Lentivirus is a transcription factor reporter system that delivers a quantitative readout of thyroid hormone receptor pathway activity in mammalian cells. The construct places a fluorescent or luminescent reporter (GFP, RFP, or firefly luciferase) under the control of tandem consensus DR4 response elements coupled to a minimal promoter and an optimized upstream mini-enhancer that amplifies signal-to-noise. A constitutive drug selection marker (Puromycin or Blasticidin) enables straightforward establishment of stable reporter cell lines. Stable lentiviral integration provides consistent reporter expression and supports readout by fluorescence microscopy, flow cytometry, or luminometry. Supplied as high-titer particles purified by PEG precipitation and sucrose gradient centrifugation, the product is suited to studying thyroid hormone responses in primary and difficult-to-transfect cells.
About This Product
This reporter lentivirus places a EGFP, Firefly Luc, mCherry, Renilla Luc, GFP, RFP, Luc reporter gene under the control of tandem consensus response elements specific for the Thyroid stimulating hormone receptor (TSHR) pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
Research budgets are tight — we get it. That's why we've put together a fresh round of exclusive promotions designed to help you stock up on the reagents, kits, and consumables your lab depends on, without stretching your budget.
🔬 What's on offer right now:
10% Off Pre-Designed siRNA Sets
20% Off Transmembrane Proteins
50% Off Lab Consumables + Free Shipping
$99 Pipette Filler Promotion Package
BlasTaq 2X qPCR MasterMix - 50% OFF Limited Time Offer
DENARASE® Endonuclease — 10% Off One Order
10% OFF CELL LINES-Limited-Time Offer
