| Field | Specification |
|---|---|
| Species |
AGS cells are a human gastric adenocarcinoma cell line derived from the stomach tissue of a 54-year-old Caucasian female. They are extensively used in biomedical research focused on gastric cancer, including studies on cancer cell biology, pathogenesis, and drug testing.
The AGS cell line exhibits epithelial-like morphology and is characterized by its aggressive growth pattern and tumorigenic potential in vivo. These cells are commonly used as a model to study the molecular and cellular mechanisms underlying gastric carcinogenesis, including the influence of Helicobacter pylori infection, a well-known risk factor for gastric cancer. AGS cells provide a robust system to explore the interactions between gastric cancer cells and H. pylori, especially regarding how bacterial factors affect cancer cell proliferation, apoptosis, and inflammatory responses.
AGS cells are also valuable for examining the gastric epithelial barrier's response to various stimuli, including inflammatory cytokines, and for studying signaling pathways implicated in gastric cancer, such as those involving NF-kB, Wnt, and MAPK. Their utility extends to the assessment of new therapeutic agents, where they are used to evaluate the efficacy and mechanisms of action of anticancer drugs, targeted therapies, and natural compounds with potential anti-cancer properties.
Furthermore, AGS cells are often employed in studies aimed at understanding the genetic and epigenetic alterations in gastric cancer, offering insights into potential diagnostic markers and therapeutic targets for this challenging and frequently fatal disease.
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- Protein expression: p53 positive
- Tumorigenic: Yes, in athymic BALB/c mice
- Viruses: This cell line may release Parainfluenzavirus Type 5 (formerly known as Simian Virus 5). The virus interferes with Interferon-signalling within the cell line by degradation of STAT1.
- Karyotype: Modal number = 47, range = 39 to 92
- cultureMedium: DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 3.7 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)
- supplements: Supplement the medium with 10% FBS
- dissociationReagent: Accutase
- doublingTime: 24 to 48 hours
- subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
- seedingDensity: 1 x 104 cells/cm2 will result in a confluent monolayer within 3to5 days.
- fluidRenewal: 2 to 3 times per week
- freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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