| Field | Specification |
|---|---|
| Target | |
| Alternative names | Potassium-transporting ATPase alpha chain 1; Gastric H(+)/K(+) ATPase subunit alpha; Proton pump; ATP4A |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human ATP4A, which shares 96.2% amino acid (aa) sequence identity with mouse and rat ATP4A. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects potassium-transporting ATPase alpha chain 1 (gene ATP4A) in human, mouse and rat samples and is validated for Western blot, IHC and flow cytometry. The predicted molecular weight is 114.1 kDa, and the supplier reports an observed band at about 130 kDa.
ATP4A encodes the catalytic α chain of the stomach H+/K+-ATPase, the proton pump of stomach parietal cells. It exchanges cytosolic H+ for luminal K+ to acidify the stomach and is the target of proton pump inhibitors; it is also a major autoantigen in autoimmune gastritis.
| Target | Potassium-transporting ATPase alpha chain 1 (gene ATP4A; UniProt P20648, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 114.1 kDa |
| Observed MW | 130 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human, Rat) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): rat testis tissue, rat stomach tissue, mouse testis tissue, mouse stomach tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human stomach tissue, rat stomach tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with a confirmed signal (flow cytometry): Daudi cells.
Immunogen
Synthetic peptide from the N-terminal region of human ATP4A. Sequence identity with the mouse and rat orthologs: 96.2%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places potassium-transporting ATPase alpha chain 1 in the apical cell membrane. Tissue expression noted by UniProt (human): Expressed in gastric parietal cells (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
