Anti-Bmi1 Rabbit Monoclonal Antibody

Overview

Anti-Bmi1 Rabbit Monoclonal Antibody is an antibody targeting BMI1. Common applications include WB, IHC, ICC, IF. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: IHE-2; isotype: Rabbit IgG; reactivity: Human,Rat; observed MW: 40 kDa; calculated MW: 36949 MW.

Boster Bio Anti-Bmi1 Rabbit Monoclonal Antibody catalog # M00313. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Rat.

Key elements and design rationale

• Target: BMI1 — Polycomb complex protein BMI-1
• Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: IHE-2; Isotype: Rabbit IgG
• Species reactivity: Human,Rat
• Molecular weight guidance: Observed: 40 kDa; Calculated: 36949 MW

Biological background

Protein function (datasheet): Component of a Polycomb group (PcG) multiprotein PRC1- like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2. .

Cellular localization (datasheet): Nucleus. Cytoplasm.

Tissue details (datasheet): Ubiquitously expressed in various human primary cells and tumor cell lines. .

Research relevance and current trends

• Commonly studied in contexts related to Cancer,Cancer Susceptibility,Cell Biology,Cell Cycle,Cell Differentiation,2339,Chromatin Remodeling,Epigenetics and Nuclear Signaling,Hematopoietic Progenitors,Oncoproteins,Oncoproteins/Suppressors,Proto-Oncogenes,Stem Cells,Transcription.
• Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
• Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).

Common research applications

• Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
• Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
• Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.

Notes for experimental interpretation

• Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
• Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.

As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.