Anti-Human P2Y11 Receptor Antibody

Overview

Anti-Human P2Y11 Receptor Antibody is an antibody targeting P2Y purinoceptor 11, P2RY11 Polyclonal raised in Rabbit (Unconjugated). This antibody is commonly used in IC, IF, IFC, IP, WB to detect, localize, or compare expression of the target across samples.

Key elements and design rationale

• Target: P2Y purinoceptor 11, P2RY11 (also reported as P2Y purinoceptor 11, P2RY11).
• Immunogen/epitope region: Intracellular, C-terminus.
• Homology note: Human only Not recommended for use with rat and mouse samples (informative for cross-species interpretation).
• Species reactivity (as provided): Human.
• Cited use: IHC, ICC (literature use does not guarantee performance in every setup).
• Lot quality control (as provided): Western blot analysis.
• Peptide confirmation: Confirmed by amino acid analysis and mass spectrometry.
• Blocking peptide: Available for antigen preadsorption control where appropriate.

These attributes help researchers interpret whether signal reflects the intended target in a given assay and sample context.

Biological background

The P2Y receptors belong to the G-protein coupled receptor superfamily. They mediate the actions of the extracellular nucleotides (ATP, ADP, UTP and UDP). Eight functional mammalian P2Y receptors have been described: P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13, and the UDP-glucose receptor, now renamed P2Y14.1-3 The human P2Y11 receptor is unique among the P2Y receptors since it is dually coupled to phospholipase C and adenylyl cyclase.4-5 P2Y11 receptor is highly expressed in the spleen and to a much lower extent in platelets.Expression of P2Y11 receptor was also shown in endothelial cells.6 In granulocytes HL-60), P2Y11 receptor is thought to have a role in granulocytic differentiation.4

Research relevance and current trends

• Comparing target expression across perturbations, genotypes, or treatment conditions.
• Interpreting localization shifts alongside pathway or phenotypic readouts.
• Using orthogonal controls (KO/KD, peptide competition, isotype concepts) to support conclusions.

Common research applications

• Western blot (WB): compare target abundance/size across lysates and conditions; consider isoforms/PTMs.
• Immunofluorescence/ICC: assess subcellular localization and co-localization with markers in cells or sections.
• Flow cytometry (direct/indirect): quantify target-positive populations and shifts in expression across subsets.
• Immunoprecipitation (IP): enrich the target for downstream detection or complex analysis (context-dependent).

Interpretation typically benefits from comparing matched sample sets (e.g., treated vs control, WT vs KO/KD) and using orthogonal readouts where feasible.

Notes for experimental interpretation

• Isoforms and post-translational modifications can shift apparent molecular weight or epitope accessibility across samples.
• Cross-species signal may depend on epitope conservation; consult the provided homology note when selecting models.
• Permeabilization, fixation, and antigen retrieval can change accessibility of intracellular vs extracellular epitopes.
• Conceptual control: antigen preadsorption (blocking peptide) can help assess signal dependence on the immunogen region.
• Provided control suggestions: Negative control: BLP-PR015.
• Application notes: see product-specific dilution/usage notes and control concepts provided in the dataset.

Application abbreviations: CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot. Species abbreviations: H- Human, M- Mouse, R- Rat.

Recommended controls: Blocking peptide: BLP-PR015; Negative control: BLP-PR015.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.