Anti-NaV1.8 (SCN10A) (extracellular) Antibody

Overview

Anti-NaV1.8 (SCN10A) (extracellular) Antibody is an antibody targeting PN3, SNS, Sodium channel protein type 10 subunit alpha Polyclonal raised in Rabbit (Unconjugated). This antibody is commonly used in WB to detect, localize, or compare expression of the target across samples.

Key elements and design rationale

• Target: PN3, SNS, Sodium channel protein type 10 subunit alpha (also reported as PN3, SNS, Sodium channel protein type 10 subunit alpha).
• Immunogen/epitope region: 1st extracellular loop, domain III..
• Homology note: Mouse - identical; human - Not recommended for use with human samples (informative for cross-species interpretation).
• Species reactivity (as provided): Rat, Mouse.
• Specificity statement (as provided): Not recommended for use with human samples..
• Lot quality control (as provided): Western blot analysis.
• Peptide confirmation: Confirmed by amino acid analysis and mass spectrometry.
• Blocking peptide: Available for antigen preadsorption control where appropriate.

These attributes help researchers interpret whether signal reflects the intended target in a given assay and sample context.

Biological background

Voltage-gated Na+ channels (NaV) are essential for the generation of action potentials and for cell excitability.1 NaV channels are activated in response to depolarization and selectively allow flow of Na+ ions. To date, nine NaV α subunits have been cloned and named NaV1.1-1.9.2-3 The NaV channels are classified into two groups according to their sensitivity to tetrodotoxin (TTX): TTX-sensitive and TTX-resistant channels.4-5b Expression of the α subunit isoform is developmentally and tissue specific.Two TTX-resistant NaV channels are expressed in dorsal root ganglion (DRG) neurons, NaV1.8 and NaV1.9. The NaV1.8 channel (also called SCN10A, SNS and PN3) is mainly expressed in small-diameter DRG neurons.4-6 TTX-resistant channels have been suggested to play an important role in nociceptive transmission.Recently, involvement of NaV1.8 in multiple sclerosis (MS) was suggested due to up-regulation of both mRNA and protein in Purkinje cells of MS patients and also in animal models.6

Research relevance and current trends

• Mapping receptor/channel localization across neuronal subtypes and subcellular compartments.
• Linking trafficking or surface expression changes to activity-dependent signaling and plasticity.
• Using KO/KD or blocking-peptide concepts to strengthen antibody-based target assignment.

Common research applications

• Western blot (WB): compare target abundance/size across lysates and conditions; consider isoforms/PTMs.

Interpretation typically benefits from comparing matched sample sets (e.g., treated vs control, WT vs KO/KD) and using orthogonal readouts where feasible.

Notes for experimental interpretation

• Isoforms and post-translational modifications can shift apparent molecular weight or epitope accessibility across samples.
• Cross-species signal may depend on epitope conservation; consult the provided homology note when selecting models.
• Permeabilization, fixation, and antigen retrieval can change accessibility of intracellular vs extracellular epitopes.
• Conceptual control: antigen preadsorption (blocking peptide) can help assess signal dependence on the immunogen region.
• Provided control suggestions: Negative control: BLP-SC028.
• Application notes: see product-specific dilution/usage notes and control concepts provided in the dataset.

Application abbreviations: CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot. Species abbreviations: H- Human, M- Mouse, R- Rat.

Recommended controls: Blocking peptide: BLP-SC028; Negative control: BLP-SC028.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.