| Field | Specification |
|---|---|
| Species |
Human Gingival Fibroblasts (hGF) are primary cells derived from the connective tissue of the gingiva, or gum tissue, in the oral cavity. These fibroblasts play a critical role in maintaining the structural integrity of the gingival tissue by producing extracellular matrix components, including collagen, elastin, and glycosaminoglycans. Their ability to proliferate and migrate is essential for wound healing, tissue repair, and the response to periodontal disease. In addition to their structural roles, hGF are involved in inflammatory responses within the gingiva, interacting with various immune cells and mediating the release of cytokines and growth factors. This makes them a key cellular model for studying oral health, periodontal disease, and tissue regeneration.
hGF cells are widely used in research focused on oral biology, particularly in understanding the pathophysiology of periodontal diseases, where the interaction between fibroblasts and pathogenic bacteria like *Porphyromonas gingivalis* is of significant interest. These cells are also utilized in tissue engineering and regenerative medicine, especially in developing therapies for gingival and periodontal defects. Their response to different biomaterials, growth factors, and extracellular matrix components is frequently studied to optimize conditions for tissue repair and regeneration in oral surgery and dental implants.
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- cultureMedium: DMEM:Ham's F12 (1:1), w: 3.1 g/L Glucose, w: 2.5 mM L-Glutamine, w: 15 mM HEPES, w: 0.5 mM Sodium pyruvate, w: 1.2 g/L NaHCO3 (Cytion article number 820400a)
- supplements: Supplement the medium with 10% FBS, 10 ng/mL bFGF, 10 microgram/L Insulin
- dissociationReagent: Accutase
- subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
- freezeMedium: As a cryopreservation medium, use 90% FBS + 10% DMSO to maintain viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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