| Field | Specification |
|---|---|
| Species |
Virus susceptibility: Baculoviruses, Autographa californica (MNPV), St. Louis encephalitis (SLE)
- cultureMedium: Sf-900™ III SFM (Thermofisher, Cat-No. 12658019)
- supplements: Supplement the medium with 0% FBS to enhance proliferation if needed
- dissociationReagent: Accutase
- subculturing: Seeding after thawing 5x105/ml. Incubate at 27°C-28°C, without CO2, on a shaker with agitation of 90–110 rpm in Erlenmeyerflask. Keep at a cell density of 3-8x105/ml. Maximum cell density possible is 1-4x106 cells/ml.
- seedingDensity: 1 x 104 cells/cm2. Incubate between 26 to 30 degree Celsius in a non-humidified, ambient air-regulated incubator. Use cell culture flasks with filter caps or loosen caps to allow for oxygen exchange.
- fluidRenewal: 2 to 3 times per week
- freezeMedium: As a cryopreservation medium, use complete cell culture medium (including FBS) + 10% DMSO for adequate post-thaw viability.
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