Sf9 cell

SKU:BHC11100216
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Overview
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Sf9 cell is a cell line (Female). It is commonly used as an in vitro model for 1 research. Growth characteristics: Adherent, Round, attached, epitheloid. Supplied as cryopreserved cells with accompanying batch CoA and quality-control documentation.

Species Fall armyworm
Morphology Round, attached, epitheloid
Growth Properties Adherent
Tissue Ovary
Available Options

This cell line is available in the U.S. For non-profit users, please sign and submit the Non-Profit Supply Agreement to orders@biohippo.com before placing an order. For commercial users, please complete the CLEAR Form before ordering, as additional usage fees may apply based on the intended use. For further details, please contact orders@biohippo.com. Products ship after the required agreement is completed; typical delivery is 2–3 business days. Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10^6 cells for adherent lines or 5 × 10^6 cells for suspension lines (refer to the batch CoA for details).

Options selector
Catalog no. Size
604328 1 cryovial
Field Specification
Species Fall armyworm
Sf9 cells are clonal isolates derived from the Spodoptera frugiperda Sf21 cell line (IPLB-Sf-21-AE). They are commonly used in insect cell culture for recombinant protein production using baculovirus expression systems. Sf9 cells are epithelial in morphology and were cloned from the pupal ovarian tissue of the fall armyworm. One of the key characteristics of Sf9 cells is their small, regular size. They are also suitable for transfection, plaque assay/purification, amplification of high-titer stocks, and expression of recombinant proteins. The Sf9 insect cell line can be maintained in attached and suspended cultures, and do not require serum or CO2 to grow. They are considered Biosafety Level 1 and are usually grown in a 26-28 degree celsius incubator. Sf9 cells/baculovirus expression systems are widely used for high-level protein expression, often for purification, but proteins may also be functionally expressed in the defined Sf9 cell environment. The size of infected Sf9 cells is generally 17-30 microns in diameter. The Sf9 cell line is distinct from the Sf21 cell line in that it is a clonal isolate with a smaller and more regular size, while Sf21 cells are more disparate in size and form monolayers and plaques that are more irregular. Some Sf9 cell lines may harbor a negative sense Rhabdovirus called Spodoptera frugiperda rhabdovirus (SfRV), although not all tested Sf9 cells appear to be infected with this virus. The genome size of Sf9 has been estimated to be 451 Mbp with a G+C content of 36.53%.

SKU:BHC11100216

Virus susceptibility: Baculoviruses, Autographa californica (MNPV), St. Louis encephalitis (SLE)

  • cultureMedium: Sf-900™ III SFM (Thermofisher, Cat-No. 12658019)
  • supplements: Supplement the medium with 0% FBS to enhance proliferation if needed
  • dissociationReagent: Accutase
  • subculturing: Seeding after thawing 5x105/ml. Incubate at 27°C-28°C, without CO2, on a shaker with agitation of 90–110 rpm in Erlenmeyerflask. Keep at a cell density of 3-8x105/ml. Maximum cell density possible is 1-4x106 cells/ml.
  • seedingDensity: 1 x 104 cells/cm2. Incubate between 26 to 30 degree Celsius in a non-humidified, ambient air-regulated incubator. Use cell culture flasks with filter caps or loosen caps to allow for oxygen exchange.
  • fluidRenewal: 2 to 3 times per week
  • freezeMedium: As a cryopreservation medium, use complete cell culture medium (including FBS) + 10% DMSO for adequate post-thaw viability.

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Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

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