| Field | Specification |
|---|---|
| Species |
- Surface antigens: Blood Type A, Rh +, carcinoembryonic antigen (CEA) and TAG 72
- Oncogenes: myc +, erb-B2 +
- Tumorigenic: Yes, in semisolid medium
- Mutational profile: Mutation: MSH6, p.Lys1358fs*2 (c.4065_4066insTTGA), heterozygous; Mutation: TP53, p.Tyr205Phe (c.614A>T), homozygous
- cultureMedium: RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
- supplements: Supplement the medium with 10% FBS, 25 mM HEPES
- subculturing: Suspension cells: Remove cells from substrate by pipetting with fresh medium. To obtain single cells, pass the suspension several times through a 22 gauge needle and dispense into new flasks.
- fluidRenewal: 2 times per week
- freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Research budgets are tight — we get it. That's why we've put together a fresh round of exclusive promotions designed to help you stock up on the reagents, kits, and consumables your lab depends on, without stretching your budget.
🔬 What's on offer right now:
10% Off Pre-Designed siRNA Sets
20% Off Transmembrane Proteins
50% Off Lab Consumables + Free Shipping
$99 Pipette Filler Promotion Package
BlasTaq 2X qPCR MasterMix - 50% OFF Limited Time Offer
DENARASE® Endonuclease — 10% Off One Order