| Field | Specification |
|---|---|
| Alternative Names | Autophagy protein 5;APG5-like;Apoptosis-specific protein |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Synthesized peptide derived from human ATG5 AA range: 180-260 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® ATG5 Rabbit Polyclonal Antibody (WP17658) is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against ATG5. It has been validated for Western Blot, ELISA and reacts with Human, Mouse, Rat.
Validated Applications
| Target | ATG5 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Western Blot, ELISA |
| Purification | Affinity Purified |
| Specificity | This antibody detects endogenous levels of Human,Mouse,Rat ATG5 |
| Molecular Weight | Calculated: 30 kDa | Observed: 51 kDa |
| UniProt / Swiss-Prot | Q9H1Y0 (Human) | Q99J83 (Mouse) | Q3MQ06 (Rat) |
Recommended dilutions: WB 1:1000-2000;ELISA 1:5000-20000
Immunogen / Antigen
Synthesized peptide derived from human ATG5 AA range: 180-260
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. Several transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq, Sep 2015]
Safety & Handling
This antibody is validated for Western Blot, ELISA. Recommended dilutions: WB 1:1000-2000;ELISA 1:5000-20000
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
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