| Field | Specification |
|---|---|
| Alternative Names | CHEK1;CHK1;Serine/threonine-protein kinase Chk1;CHK1 checkpoint homolog;Cell cycle checkpoint kinase;Checkpoint kinase-1 |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Chk1 around the phosphorylation site of Ser286. AA range:256-305 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® Chk1 (Phospho Ser286) Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against Chk1. Recognizes Phospho. It has been validated for Western Blot, Immunofluorescence, ELISA and reacts with Human, Rat, Mouse.
Validated Applications
| Target | Chk1 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Rat, Mouse |
| Applications | Western Blot, Immunofluorescence, ELISA |
| Purification | Affinity Purified |
| Specificity | Phospho-Chk1 (S286) Polyclonal Antibody detects endogenous levels of Chk1 protein only when phosphorylated at S286. |
| Molecular Weight | Observed: 55 kDa |
| UniProt / Swiss-Prot | O14757 (Human) | O35280 (Mouse) |
Recommended dilutions: WB 1:500-1:2000;IF 1:200-1:1000;ELISA 1:5000;Not yet tested in other applications.
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from human Chk1 around the phosphorylation site of Ser286. AA range:256-305
Reactivity Notes
Confirmed cross-reactivity: Human, Rat, Mouse. Confirm suitability for untested species before use.
Scientific Background
The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2011]
Safety & Handling
This antibody is validated for Western Blot, Immunofluorescence, ELISA. Recommended dilutions: WB 1:500-1:2000;IF 1:200-1:1000;ELISA 1:5000;Not yet tested in other applications.
Confirmed reactivity: Human, Rat, Mouse. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
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