| Field | Specification |
|---|---|
| Alternative Names | Coagulation factor IX;Christmas factor;Plasma thromboplastin component;PTC;[Cleaved into: Coagulation factor IXa light chain;Coagulation factor IXa heavy chain] |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Synthesized peptide derived from human FA9 (light chain, Cleaved-Arg191) |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® FA9 (light chain, Cleaved-Arg191) Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against Factor IX. It has been validated for Western Blot, ELISA and reacts with Human, Mouse, Rat.
Validated Applications
| Target | Factor IX |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Western Blot, ELISA |
| Purification | Affinity Purified |
| Specificity | This antibody detects endogenous levels of Human,Mouse,Rat FA9 (light chain, Cleaved-Arg191, protein was cleaved amino acid sequence between 191-200 ) |
| Molecular Weight | Observed: 16 kDa,45 kDa |
| UniProt / Swiss-Prot | P00740 (Human) | P16294 (Mouse) | P16296 (Rat) |
Recommended dilutions: WB 1:1000-2000;ELISA 1:5000-20000
Immunogen / Antigen
Synthesized peptide derived from human FA9 (light chain, Cleaved-Arg191)
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
This gene encodes vitamin K-dependent coagulation factor IX that circulates in the blood as an inactive zymogen. This factor is converted to an active form by factor XIa, which excises the activation peptide and thus generates a heavy chain and a light chain held together by one or more disulfide bonds. The role of this activated factor IX in the blood coagulation cascade is to activate factor X to its active form through interactions with Ca+2 ions, membrane phospholipids, and factor VIII. Alterations of this gene, including point mutations, insertions and deletions, cause factor IX deficiency, which is a recessive X-linked disorder, also called hemophilia B or Christmas disease. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing. [provided by RefSeq, Sep 2015]
Safety & Handling
This antibody is validated for Western Blot, ELISA. Recommended dilutions: WB 1:1000-2000;ELISA 1:5000-20000
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
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