| Field | Specification |
|---|---|
| Alternative Names | ERN2;IRE2;Serine/threonine-protein kinase/endoribonuclease IRE2;Endoplasmic reticulum-to-nucleus signaling 2;Inositol-requiring protein 2;hIRE2p;Ire1-beta;IRE1b |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ERN2. AA range:841-890 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® IREβ Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against IREβ. It has been validated for Immunohistochemistry, Immunofluorescence, ELISA and reacts with Human, Mouse.
Validated Applications
| Target | IREβ |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse |
| Applications | Immunohistochemistry, Immunofluorescence, ELISA |
| Purification | Affinity Purified |
| Specificity | IRE1α/β Polyclonal Antibody detects endogenous levels of IRE1α/β protein. |
| Molecular Weight | Calculated: 102 kDa |
| UniProt / Swiss-Prot | Q76MJ5 (Human) | Q9Z2E3 (Mouse) |
Recommended dilutions: IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:10000;Not yet tested in other applications.
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from human ERN2. AA range:841-890
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse. Confirm suitability for untested species before use.
Scientific Background
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:The kinase domain is activated by trans-autophosphorylation. Kinase activity is required for activation of the endoribonuclease domain.,function:Induces translational repression through 28S ribosomal RNA cleavage in response to ER stress. Pro-apoptotic. Appears to play no role in the unfolded-protein response, unlike closely related proteins.,PTM:Autophosphorylated.,similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.,similarity:Contains 1 KEN domain.,similarity:Contains 1 protein kinase domain.
Safety & Handling
This antibody is validated for Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:10000;Not yet tested in other applications.
Confirmed reactivity: Human, Mouse. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
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