U-Blot® NPM (Phospho Thr95) Rabbit Polyclonal Antibody

SKU:BHA21804816
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    Overview
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    Anti-Nucleophosmin antibody (Rabbit host, polyclonal, Unconjugated). Validated for Western Blot with reactivity to Human, Rat, Mouse.
    Clonality Polyclonal
    Host Rabbit
    Reactivity Human, Rat, Mouse
    Conjugate Unconjugated
    Isotype IgG
    Applications Western Blot
    Options selector
    Catalog no. Size
    WP10433-50 50 uL
    WP10433-100 100 uL
    Available Options

    Select the variant that best fits your experiment. Availability and lead time may vary by option.

    • Options: Size: 50 uL
    • Lead time: options listed in "Availability Content"; other statuses may take longer.
    • Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
    • Shipping: cold-chain shipment (typically with ice packs).
    • Upon receipt: store at recommended temperature as soon as possible.
    • Sales terms and conditions: Please review prior to ordering.
    Field Specification
    Alternative Names Nucleophosmin;NPM;Nucleolar phosphoprotein B23;Nucleolar protein NO38;Numatrin
    Antigen
    • NPM (Thr95)
    Cellular Localization Nucleus, nucleolus . Nucleus, nucleoplasm . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Generally nucleolar, but is translocated to the nucleoplasm in case of serum starvation or treatment with anticancer drugs. Has been found in the cytoplasm in patients with primary acute myelogenous leukemia (AML), but not with secondary AML. Can shuttle between cytoplasm and nucleus. Co- localizes with the methylated form of RPS10 in the granular component (GC) region of the nucleolus. Colocalized with nucleolin and APEX1 in nucleoli. Isoform 1 of NEK2 is required for its localization to the centrosome during mitosis.
    Clonality
    • Polyclonal
    Concentration 1 mg/ml
    Conjugate
    • Unconjugated
    Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
    Gene ID 4869 (Human) | 18148 (Mouse) | 25498 (Rat)
    Host Rabbit
    Immunogen Synthesized phosho peptide around human NPM (Thr95)
    Isotype
    • IgG
    Molecular Weight Observed: 37 kDa
    Product Type
    • Polyclonal Antibody
    • Primary Antibodies
    Reactivity
    • Human
    • Rat
    • Mouse
    Storage -15°C to -25°C/1 year(Do not lower than -25°C)
    Target Nucleophosmin
    Antibody

    Product Overview

    U-Blot® NPM (Phospho Thr95) Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against Nucleophosmin. Recognizes Phospho. It has been validated for Western Blot and reacts with Human, Rat, Mouse.

    Validated Applications

    Target Nucleophosmin
    Host Rabbit
    Clonality Polyclonal
    Isotype IgG
    Conjugate Unconjugated
    Reactivity Human, Rat, Mouse
    Applications Western Blot
    Purification Affinity Purified
    Specificity This antibody detects endogenous levels of Human NPM (phospho-Thr95)
    Molecular Weight Observed: 37 kDa
    UniProt / Swiss-Prot P06748 (Human) | Q61937 (Mouse) | P13084 (Rat)

    Recommended dilutions: WB 1:1000-2000

    Immunogen / Antigen

    Synthesized phosho peptide around human NPM (Thr95)

    Reactivity Notes

    Confirmed cross-reactivity: Human, Rat, Mouse. Confirm suitability for untested species before use.

    Scientific Background

    This gene encodes a phosphoprotein which moves between the nucleus and the cytoplasm. The gene product is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners have been characterized, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in this gene are associated with acute myeloid leukemia. More than a dozen pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Nov 2009]

    Safety & Handling

    For Research Use Only (RUO). Not for diagnostic or therapeutic use. Store as indicated: -15°C to -25°C/1 year(Do not lower than -25°C)
    What applications is this antibody validated for?

    This antibody is validated for Western Blot. Recommended dilutions: WB 1:1000-2000

    What species does it react with?

    Confirmed reactivity: Human, Rat, Mouse. Other species should be validated by the user.

    Is this antibody conjugated?

    Conjugate: Unconjugated.

    How should it be stored?

    Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)

    Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.

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