U-Blot® Sp3/4 Rabbit Polyclonal Antibody

SKU:BHA21809343
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    Overview
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    Anti-Sp3/4 antibody (Rabbit host, polyclonal, Unconjugated). Validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA with reactivity to Human, Mouse, Rat.
    Clonality Polyclonal
    Host Rabbit
    Reactivity Human, Mouse, Rat
    Conjugate Unconjugated
    Isotype IgG
    Applications Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
    Options selector
    Catalog no. Size
    WP14550-50 50 uL
    WP14550-100 100 uL
    Available Options

    Select the variant that best fits your experiment. Availability and lead time may vary by option.

    • Options: Size: 50 uL
    • Lead time: options listed in "Availability Content"; other statuses may take longer.
    • Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
    • Shipping: cold-chain shipment (typically with ice packs).
    • Upon receipt: store at recommended temperature as soon as possible.
    • Sales terms and conditions: Please review prior to ordering.
    Field Specification
    Alternative Names SP3;Transcription factor Sp3;SPR-2;SP4;Transcription factor Sp4;SPR-1
    Antigen
    • Transcription factor Sp3/4
    Cellular Localization Nucleus. Nucleus, PML body. Localizes to the nuclear periphery and in nuclear dots when sumoylated. Some localization in PML nuclear bodies.
    Clonality
    • Polyclonal
    Concentration 1 mg/ml
    Conjugate
    • Unconjugated
    Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
    Gene ID 6670;6671 (Human) | 20687;20688 (Mouse)
    Host Rabbit
    Immunogen The antiserum was produced against synthesized peptide derived from human SP3/4. AA range:671-720
    Isotype
    • IgG
    Molecular Weight Observed: 82 kDa
    Product Type
    • Polyclonal Antibody
    • Primary Antibodies
    Reactivity
    • Human
    • Mouse
    • Rat
    Storage -15°C to -25°C/1 year(Do not lower than -25°C)
    Target Sp3/4
    Antibody

    Product Overview

    U-Blot® Sp3/4 Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against Sp3/4. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA and reacts with Human, Mouse, Rat.

    Validated Applications

    Target Sp3/4
    Host Rabbit
    Clonality Polyclonal
    Isotype IgG
    Conjugate Unconjugated
    Reactivity Human, Mouse, Rat
    Applications Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
    Purification Affinity Purified
    Specificity Sp3/4 Polyclonal Antibody detects endogenous levels of Sp3/4 protein.
    Molecular Weight Observed: 82 kDa
    UniProt / Swiss-Prot Q02447;Q02446 (Human)

    Recommended dilutions: WB 1:500-1:2000;IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:10000;Not yet tested in other applications.

    Immunogen / Antigen

    The antiserum was produced against synthesized peptide derived from human SP3/4. AA range:671-720

    Reactivity Notes

    Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.

    Scientific Background

    This gene belongs to a family of Sp1 related genes that encode transcription factors that regulate transcription by binding to consensus GC- and GT-box regulatory elements in target genes. This protein contains a zinc finger DNA-binding domain and several transactivation domains, and has been reported to function as a bifunctional transcription factor that either stimulates or represses the transcription of numerous genes. Transcript variants encoding different isoforms have been described for this gene, and one has been reported to initiate translation from a non-AUG (AUA) start codon. Additional isoforms, resulting from the use of alternate downstream translation initiation sites, have also been noted. A related pseudogene has been identified on chromosome 13. [provided by RefSeq, Feb 2010]

    Safety & Handling

    For Research Use Only (RUO). Not for diagnostic or therapeutic use. Store as indicated: -15°C to -25°C/1 year(Do not lower than -25°C)
    What applications is this antibody validated for?

    This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: WB 1:500-1:2000;IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:10000;Not yet tested in other applications.

    What species does it react with?

    Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.

    Is this antibody conjugated?

    Conjugate: Unconjugated.

    How should it be stored?

    Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)

    Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.

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