| Field | Specification |
|---|---|
| Alternative Names | TNNI3;TNNC1;Troponin I;cardiac muscle;Cardiac troponin I |
| Antigen | |
| Clonality | |
| Concentration | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | The antiserum was produced against synthesized peptide derived from mouse TNNI3. AA range:5-54 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® Troponin I-C Rabbit Polyclonal Antibody (WP14866) is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against Troponin I-C. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA and reacts with Mouse, Rat.
Validated Applications
| Target | Troponin I-C |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Mouse, Rat |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence, ELISA |
| Purification | Affinity Purified |
| Specificity | Troponin I-C Polyclonal Antibody detects endogenous levels of Troponin I-C protein. |
| Molecular Weight | Observed: 28 kDa |
| UniProt / Swiss-Prot | P19429 (Human) | P23693 (Rat) |
Recommended dilutions: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:5000;IF 1:50-200
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from mouse TNNI3. AA range:5-54
Reactivity Notes
Confirmed cross-reactivity: Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: tnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM).
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:5000;IF 1:50-200
Confirmed reactivity: Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
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