Western blot target validation

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2500 products
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Western blot test of (1) acid extracts of HeLa cells treated with sodium butyrate, (2) acid extracts of HeLa cells untreated, and (3) recombinant Histone H2B, using recombinant H2BK23ac antibody at 0.5 ug/ml.
Recombinant H2BK23ac Antibody
Target: Recombinant H2BK23ac
Antibodies, Primary Antibodies Western Blot, ELISA, Immunofluorescence
From $259.00
From $259.00
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Western blot of recombinant Histone H3.3 (1) and acid extracts of HeLa cells (2) using recombinant H3K79me2 antibody at 0.25 ug/ml showed a band of Histone H3 dimethylated at Lysine 79 (K79me2) in HeLa cells.
Recombinant H3K79me2 Antibody
Target: Recombinant H3K79me2
Antibodies, Primary Antibodies Western Blot, ELISA, ChIP, Immunohistochemistry
From $259.00
From $259.00
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Western blot testing of human K562 cell lysate with recombinant MyD88 antibody at 1:100 dilution. Predicted molecular weight ~33 kDa.
Recombinant MyD88 Antibody / N-Terminal
Target: Recombinant MyD88
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
$439.00
$439.00
In Stock at Manufacturer
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Western blot of A431 cell lysate using recombinant ACC antibody at 1:1000. Observed molecular weight ~260 kDa.
Recombinant ACC Antibody / Acetyl-CoA Carboxylase
Target: Recombinant ACC
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
$439.00
$439.00
In Stock at Manufacturer
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Western blot of recombinant Histone H3.3 (1) and acid extracts of HeLa cells (2) using recombinant H3K18me1 antibody at 1 ug/ml showed a band of Histone H3 monomethylated at Lysine 18 (K18me1) in HeLa cells.
Recombinant H3K18me1 Antibody
Target: Recombinant H3K18me1
Antibodies, Primary Antibodies Western Blot, ELISA
From $259.00
From $259.00
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Western blot testing of human 22RV1 cell lysate with recombinant Androgen Receptor antibody. Full length Androgen Receptor and splice variants may be observed.
Recombinant Androgen Receptor Antibody
Target: AR
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Immunofluorescence
$439.00
$439.00
In Stock at Manufacturer
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Western blot test of (1) acid extracts of HeLa cells and (2) recombinant Histone H4 using recombinant H4R3me1 antibody at 0.5 ug/ml showed a band of Histone monomethylated at Arginine 3 in HeLa cells.
Recombinant H4R3me1 Antibody
Target: Recombinant H4R3me1
Antibodies, Primary Antibodies Western Blot, ELISA, Immunofluorescence
From $259.00
From $259.00
In Stock at Manufacturer
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IHC testing of FFPE human tonsil tissue with recombinant Aurora B antibody at 1:1000.
Recombinant Aurora B Antibody
Target: Recombinant Aurora B
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
$439.00
$439.00
In Stock at Manufacturer
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Sandwich ELISA of human IgA2 using recombinant Human IgA2 antibody as the capture (100ng/well), and <a href=../tds/recombinant-human-ig-light-chains-antibody-rabbit-monoclonal-r20180btn>biotinylated anti-human light chains (kappa+lambda) antibody clone RM129</a> as the detect, followed by an alkaline phosphatase conjugated streptavidin.
Recombinant Human IgA2 Antibody (Biotin Conjugate)
Target: IgA2
Antibodies, Primary Antibodies Immunofluorescence, Immunohistochemistry, ELISA
$439.00
$439.00
In Stock at Manufacturer
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Western blot of human A431 cells using the recombinant Beta Actin antibody at a 1:1000. Predicted molecular weight ~42 kDa.
Recombinant Beta Actin Antibody (Loading Control)
Target: ACTB
Antibodies, Primary Antibodies Western Blot, Immunoprecipitation, Immunofluorescence, Immunohistochemistry, ChIP
$439.00
$439.00
In Stock at Manufacturer
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Western blot test of acid extracts of HeLa cells (1) and recombinant Histone H3.3 (2), using recombinant H3K9me3/S10p antibody at 0.01 ug/ml, showed a band of Histone H3 modified by both trimethylation at Lysine 9 and phosphorylation at Serine 10 (K9me3/S10p) in HeLa cells.
Recombinant H3K9me3/S10p Antibody
Target: Recombinant H3K9me3
Antibodies, Primary Antibodies Western Blot, ELISA
From $259.00
From $259.00
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Western Blot of recombinant histone H3.3 (1) and acid extracts of human HeLa cells (2) using recombinant H3K4me2 antibody at 0.025 ug/ml showed a band of histone H3 dimethylated at Lysine 4 (K4me2) in HeLa cells.
Recombinant H3K4me2 Antibody
Target: Recombinant H3K4me2
Antibodies, Primary Antibodies Western Blot, ELISA, Immunofluorescence
From $259.00
From $259.00
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ICC/IF of human HeLa cells using recombinant Calponin antibody (red). Actin filaments have been labeled with fluorescein phalloidin (green), and nucleus stained with DAPI (blue).
Recombinant Calponin Antibody
Target: CNN1
Antibodies, Primary Antibodies Western Blot, Immunofluorescence
$439.00
$439.00
In Stock at Manufacturer
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ICC staining of human HeLa cells using recombinant Acetyl Lysine antibody (red). Actin filaments was labeled with fluorescein phalloidin (green), and nuclei stained with DAPI (blue).
Recombinant Acetyl Lysine Antibody
Target: N/A (PTM)
Antibodies, Primary Antibodies Western Blot, Immunoprecipitation, Immunofluorescence, Immunohistochemistry, Flow Cytometry, ELISA
$475.00
$475.00
In Stock at Manufacturer
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Western blot of human A431 cells with recombinant Alpha Tubulin antibody at 1:1000. Predicted molecular weight ~50 kDa.
Recombinant Alpha Tubulin Antibody / TUBA1B (Loading Control)
Target: TUBA1B
Antibodies, Primary Antibodies Western Blot, Immunofluorescence
$439.00
$439.00
In Stock at Manufacturer
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ELISA of mouse immunoglobulins shows the recombinant Mouse Kappa Light Chain antibody reacts to the kappa light chain of mouse immunoglobulins. No cross reactivity with the lamda light chain, human IgG (κ+λ), rat IgG (κ+λ), or goat IgG (κ+λ).
Recombinant Mouse Kappa Light Chain Antibody (Biotin Conjugate)
Target: IGKC
Antibodies, Primary Antibodies ELISA
$439.00
$439.00
In Stock at Manufacturer
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Western blot of human brain lysate using recombinant Neurofilament antibody at 1:2500. Predicted molecular weight: 62-68 kDa.
Recombinant Neurofilament Antibody (light chain)
Target: NEFL
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
$439.00
$439.00
In Stock at Manufacturer
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ELISA of mouse immunoglobulins shows the recombinant Mouse IgG Fc antibody reacts to the Fc region of IgG1, IgG2a, and IgG2b, and very slightly to IgG3; no cross reactivity with IgM, IgA, IgE, human/rat/rabbit IgG.
Recombinant Mouse IgG Fc Antibody
Target: IgG Fc
Antibodies, Primary Antibodies ELISA
$439.00
$439.00
In Stock at Manufacturer
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Western blot of recombinant histone H3.3 (1) and acid extracts of human HeLa cells (2) using recombinant H3K9me1 antibody at 0.5 ug/ml shows a band of histone H3 monomethylated at Lysine 9 (K9me1) in HeLa cells.
Recombinant H3K9me1 Antibody
Target: Recombinant H3K9me1
Antibodies, Primary Antibodies Western Blot, ELISA, ChIP, Immunofluorescence
From $259.00
From $259.00
In Stock at Manufacturer
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Western blot of A431 cell lysate, untreated (-) or treated (+) with Lambda Protein Phosphatase (λPP), using recombinant phospho-ACC antibody at 1:1000.
Recombinant phospho-ACC Antibody (Ser79) / Acetyl CoA Carboxylase
Target: ACACA
Antibodies, Primary Antibodies, Phospho Antibodies Western Blot, Immunohistochemistry
$439.00
$439.00
In Stock at Manufacturer
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Western blot of human MCF7 lysate using recombinant E-Cadherin antibody at 1:1000. Expected molecular weight: 135 kDa (precursor), 80-120 kDa (mature, depending on glycosylation level).
Recombinant E-Cadherin Antibody
Target: CDH1
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
$439.00
$439.00
In Stock at Manufacturer
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Western blot test of acid extracts of HeLa cells non-treated (1) or treated with sodium butyrate (2) and recombinant Histone H3.3 (3), using recombinant H3K23ac antibody at 1 ug/ml, showed a band of Histone H3 acetylated at Lysine 23 in treated HeLa cells.
Recombinant H3K23ac Antibody
Target: Recombinant H3K23ac
Antibodies, Primary Antibodies Western Blot, ELISA, Immunofluorescence
From $259.00
From $259.00
In Stock at Manufacturer
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Western blot of recombinant histone H3.3 (1) and acid extracts of human HeLa cells (2) using the recombinant H3K4me3 antibody at 0.5 ug/ml showed a band of Histone H3 trimethylated at Lysine 4 (K4me3) in HeLa cells.
Recombinant H3K4me3 Antibody
Target: Recombinant H3K4me3
Antibodies, Primary Antibodies Western Blot, Dot Blot, ELISA
From $259.00
From $259.00
In Stock at Manufacturer
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Western blot testing of human brain lysate with recombinant NeuN antibody at 1:1000 dilution. Expected molecular weight: 46-48 kDa (two isoforms may be visualized).
Recombinant NeuN Antibody / Fox3 / N-Terminal
Target: Recombinant NeuN
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
$439.00
$439.00
In Stock at Manufacturer
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Western blot test of HeLa cell lysate, treated or untreated with anisomycin, using recombinant phospho-p38 antibody at 1:2000.
Recombinant phospho-p38 Antibody (Thr180/Tyr182)
Target: MAPK14
Antibodies, Primary Antibodies, Phospho Antibodies Western Blot, Immunohistochemistry
$439.00
$439.00
In Stock at Manufacturer
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Sandwich ELISA of human plasma using the recombinant Human IgM antibody as the capture (100ng/well), and biotinylated <a href=../tds/recombinant-human-ig-light-chains-antibody-rabbit-monoclonal-r20180btn>anti-human light chains (kappa+lambda) antibody RM129</a> as the detect, followed by an alkaline phosphatase conjugated streptavidin.
Recombinant Human IgM Antibody
Target: IgM
Antibodies, Primary Antibodies Immunofluorescence, Immunohistochemistry, ELISA
$439.00
$439.00
In Stock at Manufacturer
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Western blot of A431 cell lysate, untreated (-) or treated (+) with EGF, using recombinant phospho-EGFR antibody at 1:500. Expected molecular weight: 134-180 kDa depending on glycosylation level.
Recombinant phospho-EGFR Antibody (Tyr1173)
Target: EGFR
Antibodies, Primary Antibodies, Phospho Antibodies Western Blot, Immunohistochemistry
$439.00
$439.00
In Stock at Manufacturer
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A titer ELISA performed on purified His-tagged PCSK9 recombinant protein. A serial dilution of biotin labelled His Tag antibody was used as the primary antibody, followed by alkaline phosphatase conjugated Streptavidin.
Recombinant His Tag Antibody (Biotin Conjugate)
Target: N/A (Tag)
Antibodies, Primary Antibodies Western Blot, Immunofluorescence, Flow Cytometry, Immunohistochemistry, ELISA
$439.00
$439.00
In Stock at Manufacturer
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Western blot of human MCF-7 cell lysates using recombinant Estrogen Receptor alpha antibody at a 1:500 dilution. Predicted molecular weight ~66 kDa.
Recombinant Estrogen Receptor alpha Antibody / N-Terminal Region
Target: ESR1
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
$439.00
$439.00
In Stock at Manufacturer
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ICC of serum-starved human A431 cells non-treated or treated with Calyculin A/Okadaic Acid, using the recombinant Phosphothreonine antibody at 1:500 (PE-secondary, red) and DAPI (blue).
Recombinant Phosphothreonine Antibody
Target: N/A (PTM)
Antibodies, Primary Antibodies Western Blot, Immunoprecipitation, Immunofluorescence, Immunohistochemistry, ChIP
$485.00
$485.00
In Stock at Manufacturer
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Western blot testing of human HeLa lysate with recombinant Vimentin antibody at 1:400 dilution. Predicted molecular weight ~53 kDa.
Recombinant Vimentin Antibody / C-Terminal
Target: VIM
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
$439.00
$439.00
In Stock at Manufacturer
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Western blot test of acid extracts of HeLa cells treated (1) or non-treated (2) with Nocodazole, using recombinant phospho-Histone H2A/H4 antibody at 0.5 ug/ml, showed both Histone H2A and H4 phosphorylated at Serine 1 in HeLa cells.
Recombinant phospho-Histone H2A/H4 Antibody (Ser1)
Target: H2AC20 / H4C1
Antibodies, Primary Antibodies, Phospho Antibodies Western Blot, ELISA, Immunofluorescence
From $259.00
From $259.00
In Stock at Manufacturer
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ELISA of human immunoglobulins shows recombinant Human Ig Light Chains antibody reacts to both kappa and lambda light chains of human immunoglobulins. No cross reactivity with mouse, rat, or goat immunoglobulin light chain.
Recombinant Human Ig Light Chains Antibody
Target: IGKC / IGLC
Antibodies, Primary Antibodies Immunofluorescence, Immunohistochemistry, ELISA
$439.00
$439.00
In Stock at Manufacturer
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Western blot testing of lysate from Jurkat cells, untreated (-) or treated (+) with PHA and PMA, with recombinant PD-1 antibody at 1:100 dilution. Predicted molecular weight: 32-55 kDa depending on glycosylation level.
Recombinant PD-1 Antibody / N-Terminal
Target: Recombinant PD-1
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
$439.00
$439.00
In Stock at Manufacturer
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Western blot testing of human HeLa cell lysate with recombinant Paxillin antibody at 1:1000. Predicted molecular weight: 61/65/66 kDa (alpha/beta/gamma).
Recombinant Paxillin Antibody
Target: PXN
Antibodies, Primary Antibodies Western Blot, Immunofluorescence, Immunohistochemistry
$439.00
$439.00
In Stock at Manufacturer
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Western blot of nonreduced(-) and reduced(+) mouse IgG1 (20ng/lane), using 0.2ug/mL of recombinant Mouse IgG1 antibody. This mAb reacts to nonreduced IgG1 (~150 kDa) stronger than the reduced γ1 form (~50 kDa).
Recombinant Mouse IgG1 Antibody (Biotin Conjugate)
Target: IgG1
Antibodies, Primary Antibodies Western Blot, ELISA
$439.00
$439.00
In Stock at Manufacturer
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