Western blot target validation

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2500 products
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Western blot of acid extracts from HeLa cells untreated (-) or treated (+) with sodium butyrate using recombinant H3K27ac antibody at 1 ug/ml showed a band of Histone H3 acetylated at Lysine 27 in treated HeLa cells.
Target: Recombinant H3K27ac
Antibodies, Primary Antibodies Western Blot, ELISA, Immunofluorescence
From $259.00
Regular price From $259.00
In Stock at Manufacturer
Western blot test of acid extracts from HeLa cells treated (+) or untreated (-) with sodium butyrate, using recombinant H2A.ZK7ac antibody at 0.5 ug/ml, showed a band of Histone H2A.Z acetylated at Lysine 7 in treated HeLa cells.
Target: Recombinant H2A.ZK7ac
Antibodies, Primary Antibodies Western Blot, ELISA, Immunofluorescence
From $259.00
Regular price From $259.00
In Stock at Manufacturer
Western blot of recombinant Histone H3.3 (1) and acid extracts of HeLa cells (2) using recombinant H3K9me2 antibody at 0.5 ug/ml showed a band of Histone H3 dimethylated at Lysine 9 (K9me2) in HeLa cells.
Target: Recombinant H3K9me2
Antibodies, Primary Antibodies Western Blot, ELISA, ChIP, Immunofluorescence
From $259.00
Regular price From $259.00
In Stock at Manufacturer
Sandwich ELISA with human plasma using recombinant Human IgG4 Fc antibody as the capture, and <a href=../tds/recombinant-human-ig-light-chains-antibody-rabbit-monoclonal-r20180btn>biotinylated anti-human light chains (kappa+lambda) antibody RM129</a> as the detect, followed by an AP conjugated streptavidin.
Target: IgG4 Fc
Antibodies, Primary Antibodies ELISA
$439.00
Regular price $439.00
In Stock at Manufacturer
Western blot of human HeLa cell lysate using recombinant mTOR antibody at 1:1500. Predicted molecular weight ~280 kDa, also observed at ~220 kDa.
Target: Recombinant mTOR
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
$439.00
Regular price $439.00
In Stock at Manufacturer
ELISA of single stranded DNA using recombinant 5hmC antibody. The plate was coated with streptavidin and then biotinylated single stranded unmethylated DNA, 5-Methylcytosine (5mC) DNA, and 5-Hydroxymethylcytosine (5hmC) DNA. A serial dilution of RM236 mAb was used as the primary, and an anti-rabbit IgG AKP secondary.
Target: Recombinant 5hmC
Antibodies, Primary Antibodies Dot Blot, Immunofluorescence, Immunohistochemistry, ELISA
$530.00
Regular price $530.00
In Stock at Manufacturer
Western blot test of (1) acid extracts of HeLa cells treated with sodium butyrate, (2) acid extracts of HeLa cells untreated, and (3) recombinant Histone H4 using recombinant H4K8ac antibody at 0.5 ug/ml showed a band of Histone H4 acetylated at Lysine 8 in HeLa cells.
Target: Recombinant H4K8ac
Antibodies, Primary Antibodies Western Blot, ELISA, Immunofluorescence
From $259.00
Regular price From $259.00
In Stock at Manufacturer
Western blot testing of lysate from human HeLa cells, untreated (-) or treated (+) with Calyculin A (CalA), with recombinant phospho-EIF2A antibody at 1:200 dilution. Predicted molecular weight ~36 kDa.
Target: EIF2S1
Antibodies, Primary Antibodies, Phospho Antibodies Western Blot, Immunohistochemistry
$439.00
Regular price $439.00
In Stock at Manufacturer
Western blot testing of lysate from mouse brain tissue, untreated (-) or dephosphorylated (+) with Lambda Protein Phosphatase (lambdaPP), with recombinant phospho-GluR1 antibody at 1:200 dilution. Predicted molecular weight ~102 kDa.
Target: GRIA1
Antibodies, Primary Antibodies, Phospho Antibodies Western Blot, Immunohistochemistry
$439.00
Regular price $439.00
In Stock at Manufacturer
Western blot of Jurkat cell lysate, untreated or treated with Etoposide, using recombinant Caspase-3 antibody at 1:1000.
Target: CASP3
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
$439.00
Regular price $439.00
In Stock at Manufacturer
Western blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2) using the recombinant H3K36me2 antibody at 0.5 ug/ml showed a band of histone H3 dimethylated at Lysine 36 (K36me2) in HeLa cells.
Target: Recombinant H3K36me2
Antibodies, Primary Antibodies Western Blot, ELISA
From $259.00
Regular price From $259.00
In Stock at Manufacturer
Western blot test of acid extracts from (1) HeLa cells treated with sodium butyrate and (2) HeLa cells untreated, using recombinant H4K12ac antibody at 0.5 ug/ml, showed a band of Histone H4 acetylated at Lysine 12 in HeLa cells.
Target: Recombinant H4K12ac
Antibodies, Primary Antibodies Western Blot, ELISA, Immunofluorescence
From $259.00
Regular price From $259.00
In Stock at Manufacturer
Western blot of recombinant Histone H3.3 (1) and acid extracts of HeLa cells (2) using recombinant H3K27me3 antibody at 1 ug/ml showed a band of histone H3 trimethylated at Lysine 27 (K27me3) in HeLa cells.
Target: Recombinant H3K27me3
Antibodies, Primary Antibodies Western Blot, ELISA, Immunohistochemistry
From $259.00
Regular price From $259.00
In Stock at Manufacturer
Sandwich ELISA of human plasma using recombinant Human IgD antibody as the capture (100ng/well) and biotinylated anti-human light chains (κ+λ) antibody RM129 as the detect, followed by an alkaline phosphatase conjugated streptavidin.
Target: IgD
Antibodies, Primary Antibodies ELISA
$439.00
Regular price $439.00
In Stock at Manufacturer
Western blot testing of mouse brain lysate with recombinant GFAP antibody at 1:2500. Predicted molecular weight ~50 kDa.
Target: Recombinant GFAP
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
$439.00
Regular price $439.00
In Stock at Manufacturer
Western blot testing of mouse spleen tissue lysate using recombinant CD34 antibody at 1:100 dilution. Observed molecular weight: 41~110 kDa depending on level of glycosylation.
Target: Recombinant CD34
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
$439.00
Regular price $439.00
In Stock at Manufacturer
Sandwich ELISA with human IgG3 using recombinant Human IgG3 antibody as the capture, and <a href=../tds/recombinant-human-ig-light-chains-antibody-rabbit-monoclonal-r20180btn>biotinylated anti-human light chains (kappa+lambda) antibody RM129</a> as the detect, followed by an AP conjugated streptavidin.
Target: IgG3
Antibodies, Primary Antibodies Immunofluorescence, Immunohistochemistry, ELISA
$439.00
Regular price $439.00
In Stock at Manufacturer
IHC testing of FFPE human breast cancer tissue with recombinant p65 antibody at 1:1250.
Target: Recombinant p65
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
$439.00
Regular price $439.00
In Stock at Manufacturer
ICC staining of human HeLa cells using the recombinant Alpha Tubulin antibody at 1:200 (red) and nuclei with DAPI (blue).
Target: TUBA1B
Antibodies, Primary Antibodies Western Blot, Immunofluorescence
$439.00
Regular price $439.00
In Stock at Manufacturer
Western blot test of acid extracts from HeLa cells treated (+) or untreated (-) with sodium butyrate, using recombinant H2BK20ac antibody at 0.5 ug/ml.
Target: Recombinant H2BK20ac
Antibodies, Primary Antibodies Western Blot, ELISA, Immunofluorescence
From $259.00
Regular price From $259.00
In Stock at Manufacturer
Sandwich ELISA with human IgG4 using recombinant Human IgG4 antibody as the capture, and <a href=../tds/recombinant-human-ig-light-chains-antibody-rabbit-monoclonal-r20180btn>biotinylated anti-human light chains (kappa+lambda) antibody RM129</a> as the detect, followed by an AP conjugated streptavidin.
Target: IGHG4
Antibodies, Primary Antibodies Immunofluorescence, Immunohistochemistry, ELISA
$439.00
Regular price $439.00
In Stock at Manufacturer
ICC staining of human HeLa cells using the recombinant Beta Actin antibody at 1:200 (red) and DAPI to stain the nuclei (blue).
Target: ACTB
Antibodies, Primary Antibodies Western Blot, Immunofluorescence, Immunohistochemistry, ChIP
$439.00
Regular price $439.00
In Stock at Manufacturer
Western blot testing of human HeLa cell lysate with recombinant CD44 antibody at 1:1000. Predicted molecular weight ~81kDa.
Target: Recombinant CD44
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
$439.00
Regular price $439.00
In Stock at Manufacturer
Western blot test of (1) acid extracts of HeLa cells treated with sodium butyrate and (2) recombinant histone H3.3 using recombinant H3K56ac antibody at 1 ug/ml showed a band of Histone H3 acetylated at Lysine 56 in treated HeLa cells.
Target: Recombinant H3K56ac
Antibodies, Primary Antibodies Western Blot, ELISA, ChIP, Immunofluorescence, Immunohistochemistry
From $259.00
Regular price From $259.00
In Stock at Manufacturer
Western blot testing of human HeLa cell lysate with recombinant Synaptophysin antibody. Predicted molecular weight: 34-38 kDa.
Target: SYP
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
$439.00
Regular price $439.00
In Stock at Manufacturer
Sandwich ELISA with human IgG1 using recombinant Human IgG1 antibody as the capture, and <a href=../tds/recombinant-human-ig-light-chains-antibody-rabbit-monoclonal-r20180btn>biotinylated anti-human light chains (kappa+lambda) antibody RM129</a> as the detect, followed by an AP conjugated streptavidin.
Target: IgG1
Antibodies, Primary Antibodies Immunofluorescence, Immunohistochemistry, Flow Cytometry, ELISA
$439.00
Regular price $439.00
In Stock at Manufacturer
Western blot of extracts from human HeLa cells, untreated (-) or treated (+) with Trichostatin A (TSA), using the recombinant Acetylated Alpha Tubulin antibody at 1:1000.
Target: TUBA4A
Antibodies, Primary Antibodies Western Blot, Immunofluorescence
$439.00
Regular price $439.00
In Stock at Manufacturer
ELISA of single stranded DNA using recombinant 5mC antibody. The plate was coated with streptavidin and then biotinylated single stranded unmethylated DNA, 5-Methylcytosine (5-mC) DNA, and 5-Hydroxymethylcytosine (5-hmC) DNA. A serial dilution of RM231 was used as the primary, and an anti-rabbit IgG AKP secondary.
Target: Recombinant 5mC
Antibodies, Primary Antibodies Dot Blot, ChIP, Immunofluorescence, ELISA
$530.00
Regular price $530.00
In Stock at Manufacturer
Western blot test of (1) acid extracts of HeLa cells and (2) recombinant Histone H4 recombinant H4K5ac antibody at 0.2 ug/ml showed a band of Histone H4 acetylated at Lysine 5 in HeLa cells.
Target: Recombinant H4K5ac
Antibodies, Primary Antibodies Western Blot, ELISA, Immunofluorescence
From $259.00
Regular price From $259.00
In Stock at Manufacturer
Western blot test of (1) acid extracts of HeLa cells and (2) recombinant Histone H4, using recombinant H4K20me3 antibody at 1 ug/ml, showed a band of Histone H4 trimethylated at Lysine 20 in HeLa cells.
Target: Recombinant H4K20me3
Antibodies, Primary Antibodies Western Blot, ELISA
From $259.00
Regular price From $259.00
In Stock at Manufacturer
Western blot of A431 cell lysate, untreated (-) or treated (+) with EGF, using recombinant phospho-Stat3 antibody at 1:1000.
Target: STAT3
Antibodies, Primary Antibodies, Phospho Antibodies Western Blot, Immunohistochemistry
$439.00
Regular price $439.00
In Stock at Manufacturer
Western blot testing of recombinant His-Tag Chimeric Human antibody (0.2ug/ml) with lysate from HEK293 cells transfected (+) or untransfected (-) with a His-Tag Histone H3 DNA construct. An anti-Human IgG secondary mAb was used as detect.
Target: N/A (Tag)
Antibodies, Primary Antibodies Western Blot, ELISA
$439.00
Regular price $439.00
In Stock at Manufacturer
IHC testing of FFPE human tonsil tissue with recombinant Ki67 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Target: Recombinant Ki67
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
$439.00
Regular price $439.00
In Stock at Manufacturer
Western Blot of acid extracts from human HeLa cells untreated (-) or treated (+) with sodium butyrate using the recombinant H3K14ac antibody at 0.5 ug/ml.
Target: Recombinant H3K14ac
Antibodies, Primary Antibodies Western Blot, ELISA, ChIP, Immunofluorescence
From $259.00
Regular price From $259.00
In Stock at Manufacturer
Western blot testing of human Jurkat cell lysate with recombinant CD5 antibody at 1:1000 dilution. Observed molecular weight: 55~67 kDa depending on glycosylation level.
Target: Recombinant CD5
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
$439.00
Regular price $439.00
In Stock at Manufacturer
Western blot testing of human HEK293 cell lysate with recombinant AKT antibody at 1:10,000 dilution. Predicted molecular weight: ~56 kDa.
Target: Recombinant AKT
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
$439.00
Regular price $439.00
In Stock at Manufacturer

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