Recombinant AKT Antibody / PH domain

Overview

AKT antibody supplied as a purified reagent for WB, IHC-P in Human samples. This product is a Recombinant rabbit monoclonal antibody (host: Rabbit; isotype: Rabbit IgG) intended for research use only. The target is commonly annotated with cytoplasmic, membranous, nuclear localization context, which may inform staining patterns.

Key elements and design rationale

• Antibody identity: Rabbit Monoclonal; host Rabbit; isotype Rabbit IgG; clone RM316.
• Format and purification: format: Purified; purity: Protein A purified from animal origin-free supernatant.
• Species reactivity (reported): Human.
• Applications (listed): WB, IHC-P.
• Immunogen / epitope context: A peptide corresponding to the PH domain of human AKT1/2/3 was used as the immunogen for the AKT antibody..
• Localization: Cytoplasmic, membranous, nuclear (annotation-level guidance; cell state and isoforms can shift patterns).

These attributes help you align the antibody with the biological question (target state, sample type, and readout) while keeping interpretation grounded in appropriate controls.

Biological background

AKT is the intended antigen for this primary antibody. Reported biological context includes: Akt isoforms are overexpressed in a variety of human tumors, and, at the genomic level, are amplified in gastric adenocarcinomas (Akt1), ovarian (Akt2), pancreatic (Akt2) and breast (Akt2) cancer. The role of Akt3 is less clear, though it appears to be predominantly expressed in the brain.

Akt possesses a protein domain known as a PH domain, or Pleckstrin Homology domain, named after Pleckstrin, the protein in which it was first discovered. Subcellular localization information (Cytoplasmic, membranous, nuclear) can be useful when interpreting IF/ICC patterns and selecting compartment-enriched lysates for WB.

Research relevance and current trends

• Post-translational modification mapping: phosphorylation-site–resolved antibodies are used to connect signaling inputs to target activation states and downstream readouts.
• Signal-flow and turnover studies: researchers pair immunodetection with perturbations that modulate enzymatic activity or proteostasis to understand regulation, stability, and feedback.
• Spatial and single-cell approaches: imaging-based and cytometry workflows increasingly quantify heterogeneity and relocalization rather than only bulk abundance.

Common research applications

• Western blot (WB): compare relative abundance/isoform patterns across conditions and sample types; band shifts may reflect processing or post-translational modification.
• IHC-P: commonly used to measure relative target levels or localization changes in the context of the experimental question.

Across these readouts, differences in signal intensity, localization, or complex enrichment are typically interpreted alongside sample-matched controls and independent evidence to distinguish regulation from technical variation.

Notes for experimental interpretation

• Isoforms, cleavage products, or post-translational modifications can alter apparent molecular weight and subcellular distribution; interpret bands and staining patterns in the context of expected biology and sample preparation.
• Species differences and epitope conservation may affect binding; use matched positive controls and orthogonal evidence when comparing across organisms.
• Control concepts: include appropriate isotype and secondary-only controls (for imaging), and consider genetic perturbations (knockout/knockdown/overexpression) or independent antibodies targeting distinct epitopes to strengthen conclusions.

Epitope context is defined by the immunogen description; when available, align this with known domains, PTM sites, or family homology to anticipate potential cross-reactivity patterns. As a monoclonal antibody, binding is driven by a single epitope, which can support consistent recognition but may be sensitive to epitope masking by PTMs or conformational changes.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.