Western blot target validation

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2500 products
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Western blot testing of Topoisomerase II alpha antibody and human 1: HeLa; 2: Jurkat cell lysate; Predicted molecular weight ~174 kDa.
Topoisomerase II alpha Antibody / TOP2A
Target: TOP2A
Antibodies, Primary Antibodies Western Blot
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Western blot testing of MTCO1 antibody and Lane 1: rat heart; 2: mouse heart tissue lysate. Predicted molecular weight ~57 kDa, also observed at ~40 kDa (ref. 1).
MTCO1 Antibody
Target: MTCO1
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Immunofluorescence
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IHC staining of FFPE human liver cancer tissue with Annexin A2 antibody, HRP-secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Annexin A2 Antibody / ANXA2
Target: ANXA2
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Flow Cytometry
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Western blot testing of VEGFD antibody and Lane 1: SW620; 2: COLO320; 3: 6T-CEM; 4: HT1080 cell lysate. Expected molecular weight: 40-53 kDa (pro-form), ~21 kDa (mature/active form).
VEGFD Antibody
Target: VEGFD
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
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Western blot testing of SSX2 antibody and Lane 1: MCF-7; 2: HeLa cell lysate
SSX2 Antibody
Target: SSX2
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
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Western blot testing of IL1 beta antibody and Lane 1: Recombinant mouse IL1b partial protein 10ng; 2: 5ng; 3: 2.5ng
IL1 beta Antibody
Target: IL1
Antibodies, Primary Antibodies Western Blot
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Western blot testing of nNOS antibody and Lane 1: rat brain lysate; 2: human MCF-7 lysate; Predicted molecular weight ~160 kDa.
nNOS Antibody / NOS1
Target: NOS1
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
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Western blot testing of ERK2 antibody and Lane 1: human placenta; 2: rat liver; 3: rat brain; 4: rat thymus; 5: HeLa; 6: SMMC-7721; 7: COLO320; 8: MM231; 9: CEM
ERK2 Antibody / MAPK1
Target: ERK2
Antibodies, Primary Antibodies Western Blot
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Immunofluorescent staining of FFPE human U-2 OS cells with XAF1 antibody. HIER: steam section in pH6 citrate buffer for 20 min.
XAF1 Antibody
Target: XAF1
Antibodies, Primary Antibodies Western Blot, Immunofluorescence, Flow Cytometry
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Western blot testing of NPHS2 antibody and rat kidney tissue lysate. Predicted molecular weight ~42kDa.
NPHS2 Antibody (Podocin)
Target: NPHS2
Antibodies, Primary Antibodies Western Blot
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Western blot testing of human 1) A549, 2) HeLa, 3) Jurkat and 4) K562 cell lysate with GRP94 antibody. Predicted molecular weight ~94 kDa.
GRP94 Antibody / HSP90B1
Target: GRP94
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Immunofluorescence
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Western blot testing of IL1 beta antibody and Lane 1: recombinant human partial protein 20ng; 2: 10ng
IL1 beta Antibody
Target: IL1
Antibodies, Primary Antibodies Western Blot
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Western blot testing of Ly6AL antibody and Lane 1: rat heart; 2: rat liver tissue lysate. Predicted molecular weight ~16 kDa.
Ly6AL Antibody
Target: Ly6AL
Antibodies, Primary Antibodies Western Blot
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Western blot testing of 1) rat heart, 2) rat heart, 3) mouse heart and 4) mouse heart tissue lysate with Phospholamban antibody. Predicted molecular weight: 6/12/18/24 kDa (monomer/dimer/oligomers).
Phospholamban Antibody
Target: PLN
Antibodies, Primary Antibodies Western Blot
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Western blot testing of CXCR4 antibody and HeLa cell lysate. Predicted molecular weight ~40 kDa but may be observed at higher molecular weights due to glycosylation.
CXCR4 Antibody
Target: CXCR4
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
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Western blot testing of Podocin antibody and rat kidney tissue lysate. Predicted molecular weight ~42 kDa.
Podocin Antibody
Target: NPHS2
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
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Western blot testing of ERK2 antibody and Lane 1: rat brain; 2: rat lung; 3: rat placenta; 4: HT1080; 5: HeLa; 6: MM231; 7: Raji; 8: COLO320 cell lysate. Predicted/observed molecular weight: ~41kDa.
ERK2 Antibody / MAPK1
Target: ERK2
Antibodies, Primary Antibodies Western Blot
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Western blot testing testing of human 1) MCF-7, 2) HeLa, 3) HepG2, 4) SW620, 5) rat brain, 6) rat PC-3, 7) mouse brain and 8) mouse SP2/0 lysate with Decorin antibody. Predicted molecular weight ~40 kDa (non-glycanated), 90-140 kDa (glycanated).
Decorin Antibody
Target: DCN
Antibodies, Primary Antibodies Western Blot
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Western blot testing of c-Fos antibody and Lane 1: HT1080; 2: COLO320 cell lysate. Expected molecular weight: ~40 kDa (unmodified), 53-68 kDa (phosphorylated).
c-Fos Antibody
Target: c-Fos
Antibodies, Primary Antibodies Western Blot
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Immunofluorescent staining of FFPE human A431 cells with beta Catenin antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Beta Catenin Antibody
Target: CTNNB1
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Immunofluorescence
$449.00
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Western blot testing of SNAP25 antibody and rat brain tissue lysate. Expected molecular weight ~25 kDa.
SNAP25 Antibody
Target: SNAP25
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
$449.00
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IHC staining of frozen human placental tissue with MMP16 antibody.
MMP16 Antibody
Target: MMP16
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
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Western blot testing of Cyclin D1 antibody and Lane 1: rat testis; 2: rat ovary; 3: rat brain; 4: HeLa; 5: MM231; 6: SW620 cell lysate. Predicted molecular weight: 32-36 kDa.
Cyclin D1 Antibody
Target: D1
Antibodies, Primary Antibodies Western Blot
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Western blot testing of FGF8 antibody and rat ovary tissue lysate. Predicted molecular weight: ~25 kDa.
FGF8 Antibody
Target: FGF8
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
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Western blot testing of b-Catenin antibody and Lane 1: rat brain; 2: rat heart; 3: rat testis; 4: human MCF-7; 5: (h) HeLa; 6: (h) M453; 7: (h) M231; 8: (h) HT1080 cell lysate. Predicted molecular weight ~85 kDa, but routinely observed at 90-95 kDa.
b-Catenin Antibody
Target: b-Catenin
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Immunofluorescence
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Western blot testing of TNFAIP1 antibody and Lane 1: rat thymus; 2: HeLa; 3: COLO320; Predicted size: 36KD; Observed size: 36KD
TNFAIP1 Antibody
Target: TNFAIP1
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Immunofluorescence
$449.00
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Western blot testing of anti-Caspase-8 antibody and Lane 1: rat thymus; 2: rat liver; 3: human MCF-7; 4: (h) HeLa; 5: (h) SMMC-7721 lysate. Predicted molecular weight: ~55 kDa (pro), ~40 kDa (large + small subunit), ~20 kDa (large subunit).
Anti-Caspase-8 Antibody (large subunit)
Target: CASP8
Antibodies, Primary Antibodies Western Blot
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Western blot testing of NR1H3 antibody and Lane 1: rat liver; 2: MCF-7; 3: HeLa cell lysate
NR1H3 Antibody (LXR alpha)
Target: NR1H3
Antibodies, Primary Antibodies Western Blot
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Western blot testing of GFAP antibody and Lane 1: human U87 cell lysate; 2: mouse brain; 3: rat brain. Predicted molecular weight: ~50 kDa.
GFAP Antibody
Target: GFAP
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
$449.00
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Western blot testing of TFPI2 antibody and human lysates from Lane 1: MM453; 2: MM231; 3: HeLa; 4: HT1080; 5: Jurkat. Expected molecular weight: ~27 kDa (unmodified), 30-35 kDa (glycosylated).
TFPI2 Antibody / Tissue factor pathway inhibitor 2
Target: TFPI2
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
$449.00
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Western blot testing of Cyclin D1 antibody and Lane 1: rat testis; 2: human placenta; 3: rat brain; 4: MCF-7; 5: COLO320; 6: SW620; 7: MM231. Predicted molecular weight: 32-36 kDa.
Cyclin D1 Antibody
Target: D1
Antibodies, Primary Antibodies Western Blot, Flow Cytometry
$449.00
$449.00
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Western blot testing of 1) human HeLa and 2) rat PC-12 cell lysate with Bcl-XS antibody. Predicted/observed molecular weight: ~19 kDa.
Bcl-XS Antibody
Target: Bcl-XS
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
$449.00
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IHC staining of FFPE human intestinal cancer tissue with Cytokeratin 8 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Cytokeratin 8 Antibody
Target: KRT8
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
$449.00
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Western blot testing of NMDAR1 antibody and Lane 1: rat brain; 2: rat brain; 3: rat liver; 4: rat heart; 5: human MDA-MB-453; 6: human MDA-MB-231; 7: human HeLa lysate. Predicted molecular weight ~105 kDa.
NMDAR1 Antibody / NR1 / GRIN1
Target: NMDAR1
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
$449.00
$449.00
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Immunofluorescent staining of FFPE human HeLa cells with HSC70 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
HSC70 Antibody / HSPA8
Target: HSC70
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
$449.00
$449.00
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Western blot testing of Estrogen receptor antibody and MCF-7 lysate (human breast cancer line). Predicted molecular weight: 53~59 kDa.
Estrogen Receptor Antibody (beta)
Target: ESR2
Antibodies, Primary Antibodies Western Blot
$449.00
$449.00
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