Western blot target validation

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2500 products
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Western blot testing of human placenta lysate with NGN3 antibody. Predicted molecular weight ~23 kDa.
NGN3 Antibody / Neurogenin 3
Target: NGN3
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Immunofluorescence
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Western blot testing of human 1) T-47D, 2) PC3, 3) SW480, 4) HT-29 and 5) PANC-1 cell lysate with IFITM5 antibody. Predicted molecular weight: ~14 kDa.
IFITM5 Antibody
Target: IFITM5
Antibodies, Primary Antibodies Western Blot, Flow Cytometry, Immunohistochemistry
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Western blot testing of human 1) lung and 2) plasma lysate with Complement factor H antibody. Predicted molecular weight ~139 kDa but may be observed at a higher molecular weight due to glycosylation.
Complement factor H Antibody / CFH
Target: CFH
Antibodies, Primary Antibodies Immunohistochemistry, Western Blot, Immunofluorescence
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IHC testing of FFPE human tonsil tissue with GST pi antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
GST pi Antibody / GSTP1
Target: GSTP1
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
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Western blot testing of human 1) A549 and 2) plasma lysate with Clusterin antibody.
Clusterin Antibody / CLU / Apolipoprotein J / APO-J
Target: CLU
Antibodies, Primary Antibodies Western Blot, Flow Cytometry, Immunohistochemistry
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Western blot testing of human SW620 cell lysate with NUDT15 antibody. Predicted molecular weight ~19 kDa.
NUDT15 Antibody / Nudix hydrolase 15
Target: NUDT15
Antibodies, Primary Antibodies Flow Cytometry, Immunohistochemistry, Western Blot
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Western blot testing of human 1) HL60, 2) HeLa, 3) cerebellum, 4) SK-BR-3 and 5) mouse brain lysate with CEPT1 antibody. Predicted molecular weight ~47 kDa.
CEPT1 Antibody
Target: CEPT1
Antibodies, Primary Antibodies Immunohistochemistry, Western Blot
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IHC testing of FFPE human brain tissue with CHRM2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
CHRM2 Antibody
Target: CHRM2
Antibodies, Primary Antibodies Immunohistochemistry, Western Blot, Immunofluorescence, Flow Cytometry
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IHC testing of FFPE human testis tissue with GTPBP2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
GTPBP2 Antibody
Target: GTPBP2
Antibodies, Primary Antibodies Flow Cytometry, Immunohistochemistry, Western Blot
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Western blot testing of human HEK293 cell lysate with SEC24B antibody at 1:2000. Predicted molecular weight ~137 kDa.
SEC24B Antibody (C-Terminal Region)
Target: SEC24B
Antibodies, Primary Antibodies Western Blot
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Western blot testing of human 1) HeLa and 2) HepG2 cell lysate with Glyoxalase I antibody. Predicted molecular weight ~21 kDa.
Glyoxalase I Antibody / GLO1
Target: GLO1
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
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Western blot testing of human 1) HeLa, 2) Jurkat and 3) HL60 cell lysate with Thymidylate Synthase antibody. Predicted molecular weight ~36 kDa.
Thymidylate Synthase Antibody / TYSY
Target: TYSY
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
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Immunofluorescent staining of human brain tissue with Synaptophysin antibody (green) and DAPI nuclear stain (blue).
Synaptophysin Antibody / SYP
Target: SYP
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
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IHC testing of FFPE human cerebellum tissue with PPT1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
PPT1 Antibody
Target: PPT1
Antibodies, Primary Antibodies Immunohistochemistry, Flow Cytometry, Western Blot
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Western blot testing of human HepG2 cell lysate with FGFR4 antibody. Expected molecular weight: 88~125 kDa depending on phosphorylation and glycosylation level.
FGFR4 Antibody
Target: FGFR4
Antibodies, Primary Antibodies Immunohistochemistry, Flow Cytometry, Western Blot
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Western blot testing of 1) human Jurkat, 2) human HeLa, 3) human 293, 4) human MCF7, 5) mouse C2C12, 6) mouse NIH 3T3 and 7) rat C6 lysate with HSP70 antibody. Expected molecular weight ~70 kDa.
HSP70 Antibody / HSPA1A / HSPA1B
Target: HSP70
Antibodies, Primary Antibodies Western Blot
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IHC testing of FFPE human hepatocarcinoma with Carboxylesterase 2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Carboxylesterase 2 Antibody / CES2
Target: CES2
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Flow Cytometry
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IHC testing of FFPE human kidney tissue with SERPINA6 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
SERPINA6 Antibody
Target: SERPINA6
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Immunofluorescence
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IHC testing of FFPE human skeletal muscle tissue with PPARA antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
PPARA Antibody / PPAR alpha
Target: PPARA
Antibodies, Primary Antibodies Immunohistochemistry, Immunofluorescence, Flow Cytometry, Western Blot
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Western blot testing of human 1) kidney and 2) liver lysate with Sclerostin antibody. Predicted molecular weight ~24 kDa.
Sclerostin Antibody / SOST
Target: SOST
Antibodies, Primary Antibodies Immunohistochemistry, Western Blot
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Western blot testing of human 1) A549, 2) HUVEC, 3) liver and 4) placenta lysate with LOX-1 antibody. Predicted molecular weight: pro-form 35-50 kDa, mature form ~31 kDa.
LOX-1 Antibody / OLR1
Target: LOX-1
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
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Western blot testing of human CCRF-CEM cell lysate with PTAR1 antibody. Expected molecular weight: 46-50 kDa.
PTAR1 Antibody
Target: PTAR1
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Flow Cytometry
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Western blot testing of 1) human A549, 2) human HepG2 and 3) rat testis lysate with ALK2 antibody at 1:1000. Predicted molecular weight ~57 kDa.
ALK2 Antibody / ACVR1
Target: ALK2
Antibodies, Primary Antibodies Western Blot
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Western blot testing of 1) human HepG2, 2) human HeLa and 3) mouse liver lysate with Albumin antibody. Predicted molecular weight ~66 kDa.
Albumin Antibody / ALB
Target: ALB
Antibodies, Primary Antibodies Immunohistochemistry, Western Blot, Flow Cytometry
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Western blot testing of human 1) brain, 2) K562, 3) Jurkat, 4) HepG2 and 5) A431 lysate with TSG101 antibody at 1:2000. Predicted molecular weight ~45 kDa.
TSG101 Antibody
Target: TSG101
Antibodies, Primary Antibodies Western Blot
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Western blot testing of human 1) U-87 MG, 2) HeLa and 3) BxPC-3 cell lysate with MGEA5 antibody at 1:2000. Predicted molecular weight ~103 kDa, routinely observed at ~130 kDa (cytoplasmic form) and ~75 kDa (MGEA5s/nuclear form).
MGEA5 Antibody / OGA / O-GlcNAcase
Target: MGEA5
Antibodies, Primary Antibodies Western Blot
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IHC testing of FFPE human brain tissue with Angiotensin II Type 1 Receptor antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Angiotensin II Type 1 Receptor Antibody / AGTR1 / AT1R
Target: AGTR1
Antibodies, Primary Antibodies Immunohistochemistry, Western Blot, Flow Cytometry, Immunofluorescence
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Western blot testing of human HL60 cell lysate with HSPA5 antibody. Predicted molecular weight: ~73 kDa, but routinely observed at 70-78 kDa.
HSPA5 Antibody / GRP78 / BiP
Target: HSPA5
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
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IHC testing of FFPE human skeletal muscle tissue with MCT4 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
MCT4 Antibody / SLC16A3
Target: MCT4
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
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Western blot testing of human U-251 MG cell lysate with SH2B3 antibody at 1:2000. Predicted molecular weight ~63 kDa.
SH2B3 Antibody / LNK (N-Terminal Region)
Target: SH2B3
Antibodies, Primary Antibodies Western Blot
$205.00
$205.00
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Western blot testing of human 1) U-87 MG, 2) U-2 OS, 3) SH-SY5Y, 4) 293T, 5) HepG2 and 6) mouse brain lysate with Engrailed 1 antibody. Predicted molecular weight ~40 kDa.
Engrailed 1 Antibody / EN1
Target: EN1
Antibodies, Primary Antibodies Immunofluorescence, Western Blot
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Western blot testing of human 1) COLO 205, 2) brain, 3) HeLa, 4) MCF7 and 5) SH-SY5Y lysate with KCNH1 antibody at 1:2000. Predicted molecular weight ~111 kDa (may be observed larger than predicted due to glycosylation).
KCNH1 Antibody / EAG1 (C-Terminal Region)
Target: KCNH1
Antibodies, Primary Antibodies Western Blot
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Western blot testing of human heart lysate with NDUFA2 antibody at 1:2000. Predicted molecular weight ~11 kDa.
NDUFA2 Antibody / C-Terminal Region
Target: NDUFA2
Antibodies, Primary Antibodies Western Blot
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Western blot testing of human 1) 293T/17, 2) brain and 3) K562 lysate with SLC14A2 antibody at 1:500. Predicted molecular weight ~101 kDa.
SLC14A2 Antibody / N-Terminal Region
Target: SLC14A2
Antibodies, Primary Antibodies Western Blot
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Western blot testing of mouse EL4 cell lysate with TCIRG1 antibody at 1:2000. Predicted molecular weight ~93 kDa, routinely observed at ~116 kDa.
TCIRG1 Antibody (C-Terminal Region)
Target: TCIRG1
Antibodies, Primary Antibodies Western Blot, Flow Cytometry
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IHC testing of FFPE mouse skeletal muscle tissue with Vinculin antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Vinculin Antibody
Target: VCL
Antibodies, Primary Antibodies Western Blot, Flow Cytometry, Immunohistochemistry
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