Western blot target validation

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2500 products
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Western blot testing of human Jurkat cell lysate with CIP2A antibody at 1:2000. Predicted molecular weight: ~102 kDa, routinely observed at ~90 kDa.
CIP2A Antibody / p90 Autoantigen (Center Region)
Target: CIP2A
Antibodies, Primary Antibodies Western Blot
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Western blot testing of human 1) Jurkat, 2) U-2 0S, 3) HeLa and 4) MCF7 cell lysate with PSMC2 antibody at 1:2000. Predicted molecular weight ~49 kDa.
PSMC2 Antibody / Proteasome 19S S7 / MSS1
Target: PSMC2
Antibodies, Primary Antibodies Western Blot
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Western blot testing of human Li-7 cell lysate with IGFBP1 antibody at 1:2000. Expected molecular weight: 28-35 kDa.
IGFBP1 Antibody (Center Region)
Target: IGFBP1
Antibodies, Primary Antibodies Western Blot
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Western blot testing of 1) monkey COS7, 2) human MCF7, 3) human HCT116 and 4) human A549 cell lysates using S100A6 antibody 1:1000. Predicted molecular weight ~10 kDa.
S100A6 Antibody
Target: S100A6
Antibodies, Primary Antibodies Western Blot
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Western blot testing of human 293T/17 cell lysate with DNA Polymerase alpha antibody at 1:2000. Predicted molecular weight ~166 kDa, observed at ~180 kDa.
DNA Polymerase alpha Antibody / POLA1 (N-Terminal Region)
Target: POLA1
Antibodies, Primary Antibodies Western Blot
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Western blot testing of human 1) LNCaP, 2) PC-3 and 3) HEK293 cell lysate with OR6C3 antibody at 1:1000 dilution. Predicted molecular weight ~35 kDa.
OR6C3 Antibody
Target: OR6C3
Antibodies, Primary Antibodies Western Blot
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Western blot testing of human 1) SW480, 2) PANC-1, 3) MCF7 and 4) HeLa cell lysate with OR7C2 antibody at 1:1000. Predicted molecular weight ~35 kDa.
OR7C2 Antibody
Target: OR7C2
Antibodies, Primary Antibodies Western Blot
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Western blot testing of rat brain lysate with VGLUT2 antibody at 1:2000. Predicted molecular weight ~64 kDa.
VGLUT2 Antibody / SLC17A6 (Center Region)
Target: VGLUT2
Antibodies, Primary Antibodies Western Blot
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Western blot testing of human 1) HL60, 2) A431, 3) Jurkat and 4) mouse NIH3T3 cell lysate with METTL14 antibody at 1:2000. Expected molecular weight: 52-65 kDa.
METTL14 Antibody / N-Terminal Region
Target: METTL14
Antibodies, Primary Antibodies Western Blot
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Western blot testing of human 1) RPMI-8226, 2) A549, 3) A431, 4) HT-1080 and 5) MCF7 cell lysate with RHBDF2 antibody. Predicted molecular weight ~97 kDa.
RHBDF2 Antibody
Target: RHBDF2
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
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IHC testing of FFPE human pancreas tissue with Glucagon antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Glucagon Antibody
Target: GCG
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
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Western blot testing of human 1) HeLa, 2) MCF7, 3) SW620 and 4) A549 cell lysate with GAA antibody. Predicted molecular weight ~105 kDa.
GAA Antibody / Glucosidase alpha acid
Target: GAA
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
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IHC testing of FFPE human kidney tissue with RPS18 antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
RPS18 Antibody
Target: RPS18
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
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Western blot testing of human 1) HCT-116 and 2) MCF7 cell lysates with ESRP1 antibody. Predicted molecular weight: 68-76 kDa (multiple isoforms).
ESRP1 Antibody / Epithelial splicing regulatory protein 1
Target: ESRP1
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
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Western blot testing of human 1) heart, 2) kidney, 3) skeletal muscle and 4) SK-BR-3 lysate with SPNS2 antibody. Predicted molecular weight ~58 kDa.
SPNS2 Antibody
Target: SPNS2
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Flow Cytometry
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IHC testing of FFPE human brain tissue with Lingo1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Lingo1 Antibody
Target: Lingo1
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
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IHC testing of FFPE human placenta tissue with SGPP1 antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
SGPP1 Antibody / Sphingosine-1-phosphate phosphatase 1
Target: SGPP1
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
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Western blot testing of c-Met A) transfected (partial protein) and (B) untransfected CHO-K1 cell lysate with c-Met antibody at 1:1000. Predicted molecular weight of the transfected partial: ~38 kDa.
c-Met Antibody
Target: MET
Antibodies, Primary Antibodies Western Blot
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Western blot testing of human 1) 293 and 2) MCF7 cell lysate with IDOL antibody. Predicted molecular weight ~50 kDa.
IDOL Antibody / MYLIP
Target: IDOL
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Flow Cytometry
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Western blot testing of human 1) HEK293, 2) HT-29 and 3) U-2 OS cell lysate with ADD1 antibody at 1:2000. Predicted molecular weight ~81 kDa.
ADD1 Antibody / Alpha Adducin (Center Region)
Target: ADD1
Antibodies, Primary Antibodies Western Blot
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Immunofluorescent staining of fixed and permeabilized human T-47D cells with Catenin Beta antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
Catenin Beta Antibody / CTNNB1
Target: CTNNB1
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
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Western blot testing of 1) human heart and 2) human skeletal muscle lysate with PD-L1 antibody at 1:1000. Predicted molecular weight: 34-70 kDa depending on glycosylation level.
PD-L1 Antibody / C-Terminal Region
Target: PD-L1
Antibodies, Primary Antibodies Western Blot
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Western blot testing of lysate from human A2780 cells untreated or treated with alkaline phosphatase using phospho-CDC25A antibody. Predicted molecular weight: 59-70 kDa.
Phospho-CDC25A Antibody (pS124)
Target: Ps124
Antibodies, Primary Antibodies, Phospho Antibodies Western Blot, Immunohistochemistry
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Western blot testing of human U-87 MG cell lysate with SCN1A antibody at 1:2000. Predicted molecular weight ~229 kDa, routinely observed at ~250 kDa.
SCN1A Antibody / Nav1.1 (Center Region)
Target: SCN1A
Antibodies, Primary Antibodies Western Blot, Flow Cytometry
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Western blot testing of human 1) cerebellum and 2) spleen tissue lysate with Grancalcin antibody at 1:2000. Predicted molecular weight ~24 kDa.
Grancalcin Antibody / GCA (N-Terminal Region)
Target: GCA
Antibodies, Primary Antibodies Western Blot
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Western blot testing of 1) human K562 and 2) mouse NIH3T3 cell lysate with MUT antibody at 1:2000. Predicted molecular weight ~83 kDa.
MUT Antibody / Methylmalonyl-CoA mutase
Target: MUT
Antibodies, Primary Antibodies Western Blot
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Western blot testing of human Jurkat lysate with ATG16L1 antibody. Predicted molecular weight ~68 kDa.
ATG16L Antibody
Target: ATG16L
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Immunofluorescence
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Western blot testing of human 1) MCF7, 2) HepG2, 3) T-47D, 4) mouse cerebellum and 5) human placenta lysate with GLG1 antibody. Predicted molecular weight ~135 kDa.
GLG1 Antibody / Golgi
Target: GLG1
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
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Western blot testing of 1) mouse kidney, 2) mouse lung and 3) rat lung tissue lysate with DLL4 antibody at 1:2000. Predicted molecular weight: 75-85 kDa.
DLL4 Antibody
Target: DLL4
Antibodies, Primary Antibodies Western Blot
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Western blot testing of 1) human brain and 2) mouse brain lysate with SNCA antibody at 1:2000. Predicted molecular weight ~15 kDa.
SNCA Antibody / Alpha-Synuclein
Target: SNCA
Antibodies, Primary Antibodies Western Blot
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Western blot testing of human 1) HeLa, 2) THP1 and 3) RPMI 8226 cell lysate with GPX7 antibody at 1:2000. Predicted molecular weight ~20 kDa.
GPX7 Antibody
Target: GPX7
Antibodies, Primary Antibodies Western Blot
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Western blot testing of human 1) HeLa and 2) HepG2 cell lysate with MUT antibody at 1:2000. Predicted molecular weight ~83 kDa.
MUT Antibody / Methylmalonyl-CoA mutase / N-Terminal Region
Target: MUT
Antibodies, Primary Antibodies Western Blot
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Western blot testing of human 1) Caco-2, 2) RMPI-8226 and 3) SH-SY5Y cell lysate with CKS2 antibody at 1:2000. Predicted molecular weight ~10 kDa.
CKS2 Antibody / Cyclin-dependent kinases regulatory subunit 2 (N-Terminal Region)
Target: CKS2
Antibodies, Primary Antibodies Western Blot
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Western blot testing of 1) human skeletal muscle and 2) rat skeletal muscle lysate with MYL1 antibody at 1:2000. Predicted molecular weight ~21 kDa.
MYL1 Antibody (N-Terminal Region)
Target: MYL1
Antibodies, Primary Antibodies Western Blot
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Western blot testing of lysates from human T47D, Jurkat, untransfected CHO-K1(B) and CHO-K1 transfected with an HDAC6 fragment (A) using HDAC6 antibody at 1:1000. Predicted molecular weight: 130-160 kDa (full length), ~55 kDa (partial).
HDAC6 Antibody
Target: HDAC6
Antibodies, Primary Antibodies Western Blot
$439.00
$439.00
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ICC/IF analysis of HeLa cells, fixed fixed by anhydrous methanol at -20oC, using SIRT2 antibody at 1:50 (green) and Histone H3.1 (pSer10) antibody at 1:200 (red).
SIRT2 Antibody
Target: SIRT2
Antibodies, Primary Antibodies Western Blot, Immunofluorescence
$439.00
$439.00
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