Overview
Affinity Biosciences offers over 14,000 antibody products and 16,000 peptides, with a focus on cell signaling transduction research. Their product range spans primary antibodies, phospho-specific antibodies, secondary antibodies, antigen peptides, inhibitors, siRNA, and custom antibody services. The company uses synthetic peptide haptens and recombinant proteins to produce antibodies, enabling precise targeting of specific protein regions and isoforms. Beyond catalog products, they provide fast and cost-effective custom antibody development services, including polyclonal, phospho-specific, and modification-specific antibody production. All products are backed by comprehensive datasheets and 24/7 scientific support with a replacement or refund guarantee.
Key techniques
Affinity Biosciences employs a precision-driven approach to antibody development, starting with carefully designed synthetic peptide antigens to ensure high specificity from the source. Their proprietary production pipeline uses affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns, yielding antibodies with strong affinity and high specificity. For quality validation, they utilize a three-level QC system combining IHC tissue arrays, blocking peptide verification, and mass spectrometry — an approach aligned with the independent antibody validation strategies recommended by the International Antibody Validation Working Group (IWGAV).
Product lines
Affinity Biosciences' catalog spans the full spectrum of antibody research tools. Their primary antibodies cover all major signaling pathways and are available as rabbit polyclonal and monoclonal antibodies produced using synthetic peptide antigens and affinity purification. The phospho-specific antibody collection — their flagship line — includes 2,300+ specificities with 800 worldwide exclusives. Secondary antibodies, antigen/blocking peptides, and over 500 inhibitors and siRNAs round out the catalog. Custom services include polyclonal antibody production, phospho-specific antibody development, modification-specific antibody generation, peptide synthesis, and organic/inhibitor synthesis.
Capabilities
Affinity Biosciences combines in-house manufacturing capabilities with rigorous validation protocols to deliver research-grade antibodies trusted by academic and pharmaceutical researchers worldwide. Their 3,000 sq. meter laboratory and 10,000 sq. meter animal breeding facility support end-to-end antibody production from antigen design through purification and validation. The company has established partnerships with major antibody brands globally and supports researchers with comprehensive datasheets, application data, reference images, and 24/7 technical support backed by a product performance guarantee.
QC & documentation
Quality assurance is central to Affinity Biosciences' operations. Every antibody undergoes rigorous testing using their three-level quality control system. Most rabbit polyclonal antibodies are validated using independent antibody verification — one of the five validation strategies recommended by the International Antibody Validation Working Group (IWGAV). Their exclusive phospho-antibodies (Cat# AF7xxx, AF8xxx) are further verified through IHC tissue arrays across multiple tissue samples, blocking peptide experiments, and mass spectrometry. Original HPLC and MS reports are available for each product. All products come with comprehensive datasheets including application protocols, species reactivity, and supporting images.
Lead time & shipping
Affinity Biosciences ships globally from facilities in China and the United States. In-stock catalog antibodies typically ship within 1–3 business days. Products are shipped under temperature-controlled conditions appropriate for each product — antibodies are stored at -20°C and shipped in insulated packaging with ice packs or dry ice. The standard formulation (PBS pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol) ensures stability during transit. Custom antibody projects have defined timelines based on project scope. All orders include comprehensive product documentation and technical support.