HomeSuppliersAFFINITY BIOSCIENCES LTD
World's most complete library of phosphorylated antibodies. Known for 800 exclusive phospho-antibodies verified by IHC tissue array, blocking peptide + mass spectrometry validation. Winner of consecutive CiteAb "Supplier to Watch" awards. Ideal for cell signaling transduction research.

AFFINITY BIOSCIENCES LTD Verified partner

Affinity Biosciences is a pioneering life science company founded in 2006, specializing in high-quality antibodies for cell signaling transduction research. With R&D affiliates in the UK and US, and QC facilities in China, the company has developed over 14,000 antibody categories. Their phosphorylated antibodies — including 2,300+ phospho-specific antibodies with 800 worldwide exclusives — have become their global calling card, earning consecutive CiteAb "Supplier to Watch" awards.

Affinity Biosciences offers over 14,000 antibody products and 16,000 peptides, with a focus on cell signaling transduction research. Their product range spans primary antibodies, phospho-specific antibodies, secondary antibodies, antigen peptides, inhibitors, siRNA, and custom antibody services. The company uses synthetic peptide haptens and recombinant proteins to produce antibodies, enabling precise targeting of specific protein regions and isoforms. Beyond catalog products, they provide fast and cost-effective custom antibody development services, including polyclonal, phospho-specific, and modification-specific antibody production. All products are backed by comprehensive datasheets and 24/7 scientific support with a replacement or refund guarantee.

9001:2025
AFFINITY BIOSCIENCES LTD
1
Product line
Global · Ships worldwide via BioHippo
Why work with them

What makes AFFINITY BIOSCIENCES LTD different

Antibodies for signaling research

Affinity Biosciences offers over 14,000 antibody products and 16,000 peptides focused on cell signaling transduction research.

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The range spans primary antibodies, phospho-specific antibodies, secondary antibodies, antigen peptides, inhibitors, and siRNA for studying signaling pathways.

Phospho-specific antibody focus

The company develops phospho-specific antibodies as a core part of its catalog, including over 2,300 phospho-specific products.

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Using synthetic peptide haptens and recombinant proteins, it targets specific protein regions and isoforms, with a large number of worldwide exclusive antibodies.

Custom antibodies and support

Beyond catalog products, Affinity Biosciences provides custom antibody development services.

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These include polyclonal, phospho-specific, and modification-specific production, backed by comprehensive datasheets, 24/7 scientific support, and a replacement or refund guarantee.

Key techniques

Where they're strong

Affinity Biosciences employs a precision-driven approach to antibody development, starting with carefully designed synthetic peptide antigens to ensure high specificity from the source. Their proprietary production pipeline uses affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns, yielding antibodies with strong affinity and high specificity. For quality validation, they utilize a three-level QC system combining IHC tissue arrays, blocking peptide verification, and mass spectrometry — an approach aligned with the independent antibody validation strategies recommended by the International Antibody Validation Working Group (IWGAV).

Affinity PurificationPeptide Antigen DesignIHC Tissue Array ValidationBlocking Peptide Verification
Catalog scope

Product lines & services

Affinity Biosciences' catalog spans the full spectrum of antibody research tools. Their primary antibodies cover all major signaling pathways and are available as rabbit polyclonal and monoclonal antibodies produced using synthetic peptide antigens and affinity purification. The phospho-specific antibody collection — their flagship line — includes 2,300+ specificities with 800 worldwide exclusives. Secondary antibodies, antigen/blocking peptides, and over 500 inhibitors and siRNAs round out the catalog. Custom services include polyclonal antibody production, phospho-specific antibody development, modification-specific antibody generation, peptide synthesis, and organic/inhibitor synthesis.

Services

Primary Antibodies
Phospho-Specific Antibodies
Secondary Antibodies
Antigen Peptides
Antigen Peptides
Quality & logistics

QC, documentation & shipping

Quality control & documentation

Quality assurance is central to Affinity Biosciences' operations. Every antibody undergoes rigorous testing using their three-level quality control system. Most rabbit polyclonal antibodies are validated using independent antibody verification — one of the five validation strategies recommended by the International Antibody Validation Working Group (IWGAV). Their exclusive phospho-antibodies (Cat# AF7xxx, AF8xxx) are further verified through IHC tissue arrays across multiple tissue samples, blocking peptide experiments, and mass spectrometry. Original HPLC and MS reports are available for each product. All products come with comprehensive datasheets including application protocols, species reactivity, and supporting images.

Quality control
Performed & documented
Three-Level Phospho-Antibody ValidationLevel 1: IHC tissue array screening across multiple cell lines and tissue samples. Level 2: Blocking peptide experiments to confirm antibody specificity. Level 3: Mass spectrometry (immune-capture MS) to verify target protein identity. This triple-validation approach ensures the highest reliability for phospho-specific detection.
Independent Antibody Verification (IWGAV)Most polyclonal antibodies undergo independent antibody validation as recommended by the International Antibody Validation Working Group. Multiple independent antibodies targeting different epitopes of the same protein are tested side-by-side in Western blot, IHC, and immunofluorescence to confirm specificity and reproducibility.
Comprehensive Product DocumentationEach antibody comes with a detailed datasheet including: validated applications (WB, IHC-p, IHC-f, IF/ICC, IP, FCM), species reactivity data, recommended dilutions, immunogen information, storage conditions, and supporting images. Original HPLC purity reports and MS validation data are available upon request. Products guaranteed to work as described or replacement/refund provided.
Documentation
Available on request
Certificate of Analysis MSDS / SDS HPLC report Quality Policy

Lead time & shipping

Affinity Biosciences ships globally from facilities in China and the United States. In-stock catalog antibodies typically ship within 1–3 business days. Products are shipped under temperature-controlled conditions appropriate for each product — antibodies are stored at -20°C and shipped in insulated packaging with ice packs or dry ice. The standard formulation (PBS pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol) ensures stability during transit. Custom antibody projects have defined timelines based on project scope. All orders include comprehensive product documentation and technical support.

Catalog Products
In-stock antibodies and peptides: 1–3 business days processing. US domestic delivery: 3–5 business days. International shipping: 5–12 business days depending on destination and customs. Products shipped at -20°C with appropriate cold chain packaging. Blocking peptides available for each antibody and can be ordered simultaneously.
Custom Antibody Projects
Custom polyclonal antibody: approximately 8–12 weeks (peptide synthesis through ELISA titer assay and affinity purification). Custom phospho-specific antibody: approximately 10–14 weeks (includes phospho-peptide and non-phospho-peptide affinity column purification). Custom modification-specific antibody: timeline varies by project scope. Contact order@affbiotech.com for detailed quotes and timelines.

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COA / MSDS / QC on request
Bulk & custom pricing available