AFFINITY BIOSCIENCES LTD

Affinity Biosciences is a pioneering life science company founded in 2006, specializing in high-quality antibodies for cell signaling transduction research. With R&D affiliates in the UK and US, and QC facilities in China, the company has developed over 14,000 antibody categories. Their phosphorylated antibodies — including 2,300+ phospho-specific antibodies with 800 worldwide exclusives — have become their global calling card, earning consecutive CiteAb "Supplier to Watch" awards.

ISO9001
Antibodies
Tip: World's most complete library of phosphorylated antibodies. Known for 800 exclusive phospho-antibodies verified by IHC tissue array, blocking peptide + mass spectrometry validation. Winner of consecutive CiteAb "Supplier to Watch" awards. Ideal for cell signaling transduction research.

Overview

Affinity Biosciences offers over 14,000 antibody products and 16,000 peptides, with a focus on cell signaling transduction research. Their product range spans primary antibodies, phospho-specific antibodies, secondary antibodies, antigen peptides, inhibitors, siRNA, and custom antibody services. The company uses synthetic peptide haptens and recombinant proteins to produce antibodies, enabling precise targeting of specific protein regions and isoforms. Beyond catalog products, they provide fast and cost-effective custom antibody development services, including polyclonal, phospho-specific, and modification-specific antibody production. All products are backed by comprehensive datasheets and 24/7 scientific support with a replacement or refund guarantee.

2,300+ Phospho-Specific Antibodies
The world's most complete library of phosphorylated antibodies, including 800 exclusive specificities not available from any other supplier. Verified through IHC tissue arrays, blocking peptide validation, and mass spectrometry.

14,000+ Antibody Categories
Comprehensive catalog covering all major cell signaling pathways including Akt, AMPK, NF-κB, TGF-β, MAPK, JAK/STAT, PI3K/mTOR, and more. Applications include WB, IHC, IF/ICC, IP, and flow cytometry.

Custom Antibody Services
Polyclonal antibody development from $1,500/project and phospho-specific antibody development from $2,500/project. Full service from peptide synthesis through ELISA titer assay, with 24/7 customer support.

Key techniques

Affinity Biosciences employs a precision-driven approach to antibody development, starting with carefully designed synthetic peptide antigens to ensure high specificity from the source. Their proprietary production pipeline uses affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns, yielding antibodies with strong affinity and high specificity. For quality validation, they utilize a three-level QC system combining IHC tissue arrays, blocking peptide verification, and mass spectrometry — an approach aligned with the independent antibody validation strategies recommended by the International Antibody Validation Working Group (IWGAV).

Affinity Purification Peptide Antigen Design IHC Tissue Array Validation Blocking Peptide Verification

Product lines

Affinity Biosciences' catalog spans the full spectrum of antibody research tools. Their primary antibodies cover all major signaling pathways and are available as rabbit polyclonal and monoclonal antibodies produced using synthetic peptide antigens and affinity purification. The phospho-specific antibody collection — their flagship line — includes 2,300+ specificities with 800 worldwide exclusives. Secondary antibodies, antigen/blocking peptides, and over 500 inhibitors and siRNAs round out the catalog. Custom services include polyclonal antibody production, phospho-specific antibody development, modification-specific antibody generation, peptide synthesis, and organic/inhibitor synthesis.

Capabilities

Affinity Biosciences combines in-house manufacturing capabilities with rigorous validation protocols to deliver research-grade antibodies trusted by academic and pharmaceutical researchers worldwide. Their 3,000 sq. meter laboratory and 10,000 sq. meter animal breeding facility support end-to-end antibody production from antigen design through purification and validation. The company has established partnerships with major antibody brands globally and supports researchers with comprehensive datasheets, application data, reference images, and 24/7 technical support backed by a product performance guarantee.

Phospho-Antibody Expertise
Industry-leading phosphorylated antibody library with 2,300+ specificities including 800 worldwide exclusives (Cat# AF7xxx, AF8xxx). Three-level QC: IHC tissue array screening, blocking peptide confirmation, and mass spectrometry verification. Original MS and HPLC reports provided with each antibody.

Custom Antibody Development
End-to-end services from peptide synthesis through purification and validation. Polyclonal antibody projects from $1,500 and phospho-specific projects from $2,500. Also available: modification-specific antibodies, peptide synthesis, and inhibitor/organic synthesis. 24/7 customer support with fast turnaround times.

QC & documentation

Quality assurance is central to Affinity Biosciences' operations. Every antibody undergoes rigorous testing using their three-level quality control system. Most rabbit polyclonal antibodies are validated using independent antibody verification — one of the five validation strategies recommended by the International Antibody Validation Working Group (IWGAV). Their exclusive phospho-antibodies (Cat# AF7xxx, AF8xxx) are further verified through IHC tissue arrays across multiple tissue samples, blocking peptide experiments, and mass spectrometry. Original HPLC and MS reports are available for each product. All products come with comprehensive datasheets including application protocols, species reactivity, and supporting images.

Three-Level Phospho-Antibody Validation
Level 1: IHC tissue array screening across multiple cell lines and tissue samples. Level 2: Blocking peptide experiments to confirm antibody specificity. Level 3: Mass spectrometry (immune-capture MS) to verify target protein identity. This triple-validation approach ensures the highest reliability for phospho-specific detection.

Independent Antibody Verification (IWGAV)
Most polyclonal antibodies undergo independent antibody validation as recommended by the International Antibody Validation Working Group. Multiple independent antibodies targeting different epitopes of the same protein are tested side-by-side in Western blot, IHC, and immunofluorescence to confirm specificity and reproducibility.

Comprehensive Product Documentation
Each antibody comes with a detailed datasheet including: validated applications (WB, IHC-p, IHC-f, IF/ICC, IP, FCM), species reactivity data, recommended dilutions, immunogen information, storage conditions, and supporting images. Original HPLC purity reports and MS validation data are available upon request. Products guaranteed to work as described or replacement/refund provided.

Lead time & shipping

Affinity Biosciences ships globally from facilities in China and the United States. In-stock catalog antibodies typically ship within 1–3 business days. Products are shipped under temperature-controlled conditions appropriate for each product — antibodies are stored at -20°C and shipped in insulated packaging with ice packs or dry ice. The standard formulation (PBS pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol) ensures stability during transit. Custom antibody projects have defined timelines based on project scope. All orders include comprehensive product documentation and technical support.

Catalog Products
In-stock antibodies and peptides: 1–3 business days processing. US domestic delivery: 3–5 business days. International shipping: 5–12 business days depending on destination and customs. Products shipped at -20°C with appropriate cold chain packaging. Blocking peptides available for each antibody and can be ordered simultaneously.

Custom Antibody Projects
Custom polyclonal antibody: approximately 8–12 weeks (peptide synthesis through ELISA titer assay and affinity purification). Custom phospho-specific antibody: approximately 10–14 weeks (includes phospho-peptide and non-phospho-peptide affinity column purification). Custom modification-specific antibody: timeline varies by project scope. Contact order@affbiotech.com for detailed quotes and timelines.

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