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A-172 (A172 or A-172 MG) is a significant cell line used in neuroscience research. It originates from the brain tissue of a 53-year-old male with glioblastoma, a type of brain cancer. These cells adhere and spread on the surface of culture dishes, with a karyotype of n = 80 (80 chromosomes). A-172 cells are hypertriploid, exhibiting over 20 marker chromosomes. They have been shown to be non-tumorigenic in anti-thymocyte serum treated NIH Swiss mice. A-172 cells have a gene expression profile that highlights their mesenchymal lineage and involvement in angiogenesis.
They express genes related to mesenchymal markers (CD90, CD105, fibroblast activation protein, tenascin C) and angiogenesis inducers (VEGF, FGF2 (b), TGFb1, thrombospondin-1). Comparisons with the T98G cell line reveal differences in morphology and surface marker expression. Both cell lines show high expression of a2 smooth muscle actin. Changing the fetal serum concentration in the culture medium affects the proportion of cells expressing specific surface antigens, such as CD73 and CD105.
A-172 and T98G cell lines accurately represent glioblastomas, providing valuable tools for studying this brain tumor. Their gene expression profiles and morphological features allow for investigations into the molecular mechanisms underlying glioblastoma development and progression. Researchers can utilize A-172 cells to gain insights into glioblastoma biology and potentially identify new therapeutic targets for this devastating disease.
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- MSI status: Stable (MSS)
- Mutational profile: Has no IDH1 mutation
- cultureMedium: DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 3.7 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)
- supplements: Supplement the medium with 10% FBS
- dissociationReagent: Accutase
- doublingTime: 40 hours
- subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
- seedingDensity: 1 x 104 cells/cm2 will result in a confluent monolayer within 3 days.
- fluidRenewal: 2 to 3 times per week
- postThawRecovery: After thawing, plate the cells at 4 x 104 cells/cm2 and allow the cells to recover from the freezing process and to adhere for at least 24 to 48 hours.
- freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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