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The HNO97 cell line is derived from an oral squamous cell carcinoma, a subtype of head and neck squamous cell carcinoma (HNSCC). This cell line has been characterized by various chromosomal abnormalities, including DNA copy number gains in regions such as 3p25-pter, 3q, 5p, 9q22-qter, 10p, 10q, 11cen-p14, 20p, and 20q, alongside a significant copy number loss in the 18q region. These genetic alterations are consistent with those frequently observed in aggressive forms of HNSCC and are associated with key oncogenes involved in tumor progression, including those implicated in cell cycle regulation and proliferation. HNO97 has been extensively used in studies focused on tumor-specific targeting and peptide binding. For instance, the HNO97 cell line was instrumental in the identification and characterization of the HBP-1 peptide, which binds specifically to HNSCC cells and shows potential for use in targeted therapies. The binding kinetics of HBP-1 to HNO97 cells revealed rapid internalization, making this cell line a valuable model for investigating the efficacy of novel therapeutic agents aimed at specific molecular targets within HNSCC tumors . Furthermore, HNO97 has been utilized in biodistribution studies using tumor-bearing nude mice, where it was shown that certain peptides, like HBP-1, preferentially accumulate in HNO97 tumors, highlighting its utility in preclinical models for drug delivery and imaging studies. This cell line's genetic and molecular profile makes it an important tool in the study of oral cancer biology and the development of targeted treatments .
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- cultureMedium: DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 3.7 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)
- supplements: Supplement the medium with 10% FBS
- dissociationReagent: Accutase
- subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
- fluidRenewal: 2 to 3 times per week
- freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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