U-Blot® AKAP 220 Rabbit Polyclonal Antibody

SKU:BHA21805435
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UcallM Biosciences Inc
UcallM Biosciences Inc
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Overview
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Anti-AKAP 220 antibody (Rabbit host, polyclonal, Unconjugated). Validated for Immunohistochemistry, Immunofluorescence, ELISA with reactivity to Human, Rat.
Clonality Polyclonal
Host Rabbit
Reactivity Human, Rat
Conjugate Unconjugated
Isotype IgG
Applications Immunohistochemistry, Immunofluorescence, ELISA
Options selector
Catalog no. Size
WP10997-50 50 uL
WP10997-100 100 uL
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 50 uL
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Mfr No WP10997
Alternative Names AKAP11;AKAP220;KIAA0629;A-kinase anchor protein 11;AKAP-11;A-kinase anchor protein 220 kDa;AKAP 220;hAKAP220;Protein kinase A-anchoring protein 11;PRKA11
Antigen
  • A-kinase anchor protein 11
Cellular Localization Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasmic in premeiotic pachytene spermatocytes and in the centrosome of developing postmeiotic germ cells, while a midpiece/centrosome localization was found in elongating spermatocytes and mature sperm.
Clonality
  • Polyclonal
Concentration 1 mg/ml
Conjugate
  • Unconjugated
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 11215 (Human)
Host Rabbit
Immunogen The antiserum was produced against synthesized peptide derived from human AKAP11. AA range:1761-1810
Isotype
  • IgG
Molecular Weight Calculated: 211 kDa
Product Type
  • Polyclonal Antibody
  • Primary Antibodies
Reactivity
  • Human
  • Rat
Storage -15°C to -25°C/1 year(Do not lower than -25°C)
Target AKAP 220
Antibody

Product Overview

U-Blot® AKAP 220 Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against AKAP 220. It has been validated for Immunohistochemistry, Immunofluorescence, ELISA and reacts with Human, Rat.

Validated Applications

Target AKAP 220
Host Rabbit
Clonality Polyclonal
Isotype IgG
Conjugate Unconjugated
Reactivity Human, Rat
Applications Immunohistochemistry, Immunofluorescence, ELISA
Purification Affinity Purified
Specificity AKAP 220 Polyclonal Antibody detects endogenous levels of AKAP 220 protein.
Molecular Weight Calculated: 211 kDa
UniProt / Swiss-Prot Q9UKA4 (Human)

Recommended dilutions: IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:10000;Not yet tested in other applications.

Immunogen / Antigen

The antiserum was produced against synthesized peptide derived from human AKAP11. AA range:1761-1810

Reactivity Notes

Confirmed cross-reactivity: Human, Rat. Confirm suitability for untested species before use.

Scientific Background

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is expressed at high levels throughout spermatogenesis and in mature sperm. It binds the RI and RII subunits of PKA in testis. It may serve a function in cell cycle control of both somatic cells and germ cells in addition to its putative role in spermatogenesis and sperm function. [provided by RefSeq, Jul 2008]

Safety & Handling

For Research Use Only (RUO). Not for diagnostic or therapeutic use. Store as indicated: -15°C to -25°C/1 year(Do not lower than -25°C)
What applications is this antibody validated for?

This antibody is validated for Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:10000;Not yet tested in other applications.

What species does it react with?

Confirmed reactivity: Human, Rat. Other species should be validated by the user.

Is this antibody conjugated?

Conjugate: Unconjugated.

How should it be stored?

Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)

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