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Choosing Antibodies for Multiplex and Spatial Imaging Panels

How to choose the right clone for each target, pair antibodies with fluorophores, optimize staining order, and validate your panel before combining markers.

BI

Biohippo Inc

Spatial Biology & Imaging

| September 24, 2026 · 18 Multiplex IF Spatial Imaging Antibody Selection Panel Design FFPE Validation
Choosing Antibodies for Multiplex and Spatial Imaging Panels

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Featured Products

IHC testing of FFPE human tonsil stained with Ki-67 antibody (MKI67/2465). Required HIER: boiling tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.
Ki-67 Antibody
BHA17114960View product →
Western blot testing of Ki67 antibody and human samples 1: HeLa; 2: MCF-7; 3: COLO320; 4: HEPG2; 5: SKOV lysate. Predicted molecular weight ~350 kDa.
Ki67 Antibody
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Western blot testing of human 1) HepG2 and 2) MCF7 cell lysate with Cytokeratin 19 antibody (clone A53-B/A2.26). Predicted molecular weight ~43 kDa.
Cytokeratin 19 Antibody
BHA17111455View product →
IHC testing of FFPE human colon cancer stained with Cytokeratin 18 antibody (clone DA7).
Cytokeratin 18 Antibody
BHA17111452View product →
IHC staining of FFPE human skin tissue stained with Cytokeratin 18 antibody (clone DC10).
Cytokeratin 18 Antibody
BHA17111449View product →
IHC testing of FFPE human tonsil tissue with recombinant Ki67 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Recombinant Ki67 Antibody
BHA17103819View product →
Western blot analysis was performed using anti-PTPRC polyclonal antibody at 1ug/mL on various samples. Lane 1: RAW264.7 cell lysate Lane 2: Mouse spleen lysate Lane 3: Mouse thymus lysate
Anti-Mouse CD45/PTPRC Polyclonal Antibody
BHA21400002View product →
SDS-PAGE for Anti-Human CD45/PTPRC Nanobody (2H7)
Anti-Human CD45/PTPRC Recombinant Nanobody (2H7)
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