Autophagy & Flux Antibodies

Validated antibodies for autophagy and autophagic-flux studies — LC3B (LC3-I to LC3-II conversion), p62/SQSTM1, Beclin-1, ULK1, ATG5, ATG7 and more, for Western blot, IHC, and IF. Read LC3-II and p62 together, with the right controls (± lysosomal inhibitor), so a band change reflects real flux, not the antibody.

212Products
212 products
Image ProductProduct Type ApplicationPrice Status ACTION
IHC staining of FFPE human endometrium with ATG5 antibody (clone ATG5/2101). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
ATG5 Antibody
Target: ATG5
Antibodies, Primary Antibodies ELISA, Western Blot, Immunohistochemistry
From $259.00
From $259.00
In Stock at Manufacturer
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Flow cytometry testing of human HeLa cells with ATG7 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ATG7 antibody.
ATG7 Antibody
Target: ATG7
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Flow Cytometry
$449.00
$449.00
In Stock at Manufacturer
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IHC staining of FFPE human lung cancer with ULK1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
ULK1 Antibody
Target: ULK1
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Flow Cytometry
$449.00
$449.00
In Stock at Manufacturer
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Flow cytometry testing of human U-87 MG cells with ATG7/APG7 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ATG7/APG7 antibody.
ATG7/APG7 Antibody
Target: ATG7
Antibodies, Primary Antibodies Western Blot, Flow Cytometry, ELISA
$449.00
$449.00
In Stock at Manufacturer
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Western blot testing of human 1) K562, 2) HEK293, 3) HeLa, 4) Jurkat, 5) HepG2, 6) Raji, 7) A549 and 8) SW620 cell lysate with TFEB antibody. A major band is detected at approximately 65 kDa in all samples, which migrates higher than the predicted 53 kDa size and reflects the phosphorylated forms of TFEB. Several cell lines show a faint lower band around 60 kDa, consistent with a less phosphorylated TFEB species.
TFEB Antibody / Transcription Factor EB
Target: TFEB
Antibodies, Primary Antibodies Western Blot, ELISA
$449.00
$449.00
In Stock at Manufacturer
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Immunofluorescent staining of FFPE human A431 cells with SQSTM1 antibody (green) and DAPI (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
SQSTM1 Antibody / p62
Target: SQSTM1
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
$449.00
$449.00
In Stock at Manufacturer
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Western blot testing of human A431 cell lysate with LAMP1 antibody. This heavily glycosylated protein of 417 amino acids is visualized at up to 140 kDa.
CD107A Antibody / LAMP1
Target: CD107A
Antibodies, Primary Antibodies Western Blot
$449.00
$449.00
In Stock at Manufacturer
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Western blot testing of 1) human HepG2, 2) mouse spleen and 3) rat kidney lysate with ATG7 antibody. Predicted molecular weight: 70-80 kDa.
ATG7 Antibody
Target: ATG7
Antibodies, Primary Antibodies Western Blot
$449.00
$449.00
In Stock at Manufacturer
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Western blot testing of human HepG2 cell lysate with ATG7 antibody. Predicted molecular weight: 70-80 kDa.
ATG7 Antibody
Target: ATG7
Antibodies, Primary Antibodies Western Blot
$449.00
$449.00
In Stock at Manufacturer
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Western blot testing of human HepG2 lysate with ATG12 antibody. Expected molecular weight: ~15 kDa (ATG12) and 50-60 kDa (ATG12-ATG5 complex).
ATG12 Antibody
Target: ATG12
Antibodies, Primary Antibodies Western Blot
$449.00
$449.00
In Stock at Manufacturer
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Western blot testing of 1) human HEK293 and 2) rat PC-12 cell lysate with ULK1 antibody. Predicted molecular weight: 112-150 kDa.
ULK1 Antibody
Target: ULK1
Antibodies, Primary Antibodies Western Blot
$449.00
$449.00
In Stock at Manufacturer
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Immunofluorescent staining of human lung cancer tissue with p62 antibody (red) at 1ug/ml and DAPI counterstain. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
p62 Antibody / SQSTM1
Target: P62
Antibodies, Primary Antibodies Immunohistochemistry, Immunofluorescence, Western Blot
$449.00
$449.00
In Stock at Manufacturer
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Western blot testing of human lung lysate with TFEB antibody at 1ug/ml. Expected molecular weight: 53-60 kDa (unmodified), 65-70 kDa (modified).
TFEB Antibody
Target: TFEB
Antibodies, Primary Antibodies Western Blot, ELISA
$475.00
$475.00
In Stock at Manufacturer
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Immunofluorescent staining of FFPE human HeLa cells with Beclin 1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Beclin 1 Antibody
Target: Beclin 1
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation
$449.00
$449.00
In Stock at Manufacturer
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Flow cytometry testing of human HeLa cells with ATG7 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ATG7 antibody.
ATG7 Antibody / APG7
Target: ATG7
Antibodies, Primary Antibodies Western Blot, Flow Cytometry
$449.00
$449.00
In Stock at Manufacturer
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Western blot testing of 1) human PANC-1, 2) human HT1080, 3) human U-251, 4) rat C6 and 5) mouse testis tissue lysate with ATG5 antibody. Predicted molecular weight ATG5: ~32 kDa; ATG5/ATG12 heterodimer: ~56 kDa.
ATG5 Antibody
Target: ATG5
Antibodies, Primary Antibodies Western Blot
$449.00
$449.00
In Stock at Manufacturer
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IHC staining of FFPE human stomach cancer with p62 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
p62 Antibody / SQSTM1
Target: SQSTM1
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
$449.00
$449.00
In Stock at Manufacturer
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Western blot testing of 1) human HepG2, 2) human Jurkat, 3) human HeLa, 4) mouse brain and 5) mouse lung tissue lysate with LAMP1 antibody. This heavily glycosylated protein of 417 amino acids can be visualized at up to 140 kDa.
LAMP1 Antibody / CD107A
Target: LAMP1
Antibodies, Primary Antibodies Western Blot
$449.00
$449.00
In Stock at Manufacturer
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Western blot testing of Beclin 1 antibody and Lane 1: HeLa; 2: SW620; 3: PANC cell lysate. Predicted and Observed size: 49KD
Beclin 1 Antibody
Target: Beclin 1
Antibodies, Primary Antibodies Western Blot
$449.00
$449.00
In Stock at Manufacturer
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IHC testing of FFPE human liver tissue with ATG7 antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
ATG7 Antibody / APG7
Target: ATG7
Antibodies, Primary Antibodies Immunofluorescence, Western Blot, Immunohistochemistry
From $205.00
From $205.00
In Stock at Manufacturer
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Western blot testing of 1) huma HCT116, 2) rat brain, 3) human K562 and 4) rat C6 cell lysates using ATG5 antibody at 1:1000. Predicted molecular weight ATG5: ~32 kDa; ATG5/ATG12 heterodimer: ~56 kDa.
ATG5 Antibody
Target: ATG5
Antibodies, Primary Antibodies Western Blot
$439.00
$439.00
In Stock at Manufacturer
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Western blot analysis of APG7 antibody and recombinant protein fragment (34KD).
ATG7 Antibody
Target: ATG7
Antibodies, Primary Antibodies Western Blot, ELISA
$439.00
$439.00
In Stock at Manufacturer
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Western blot testing of Atg5 antibody at 1:4000 dilution + NIH3T3 lysate; ATG5: ~32 kDA; ATG5/ATG12 heterodimer: ~56 kDa
Atg5 Antibody (Zebrafish)
Target: Atg5
Antibodies, Primary Antibodies Western Blot, ELISA
From $205.00
From $205.00
In Stock at Manufacturer
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Western blot testing of Beclin-1 antibody at 1:2000 dilution. Lane 1: Zebrafish lysate; 2: ZF4 lysate; Predicted band size : 51 kDa.
Zebrafish Beclin-1 Antibody
Target: Beclin-1
Antibodies, Primary Antibodies Western Blot, ELISA
From $205.00
From $205.00
In Stock at Manufacturer
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Western blot testing of Atg5 antibody at 1:1000 dilution + HT-1080 lysate. ATG5: ~32 kDA; ATG5/ATG12 heterodimer: ~56 kDa
Atg5 Antibody
Target: Atg5
Antibodies, Primary Antibodies Western Blot, ELISA
From $205.00
From $205.00
In Stock at Manufacturer
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Western blot analysis of lysate from A549 cell line using p62 antibody at 1:1000. Observed molecular weight ~62 kDa.
p62 Antibody
Target: p62
Antibodies, Primary Antibodies Western Blot, ELISA
From $205.00
From $205.00
In Stock at Manufacturer
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Western blot analysis of ULK1 in mouse cerebellum tissue lysate.
ULK1 Antibody
Target: ULK1
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Flow Cytometry, ELISA
From $205.00
From $205.00
In Stock at Manufacturer
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Dot blot analysis of phospho-ULK1 antibody. 50ng of phos-peptide or nonphos-peptide per dot were spotted.
Phospho-ULK1 Antibody (pS467)
Target: ULK1
Antibodies, Primary Antibodies, Phospho Antibodies Dot Blot, ELISA
From $205.00
From $205.00
In Stock at Manufacturer
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Dot blot analysis of phospho Beclin 1 antibody. 50ng of phos-peptide or nonphos-peptide per dot were spotted.
Phospho-Beclin 1 Antibody (pS64)
Target: BECN1
Antibodies, Primary Antibodies, Phospho Antibodies Dot Blot, ELISA
From $205.00
From $205.00
In Stock at Manufacturer
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Dot blot analysis of phospho-ULK1 antibody. 50ng of phos-peptide or nonphos-peptide per dot were spotted.
Phospho-ULK1 Antibody (pS317)
Target: ULK1
Antibodies, Primary Antibodies, Phospho Antibodies Dot Blot, ELISA
From $205.00
From $205.00
In Stock at Manufacturer
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Fluorescent image of Chloroquine treated U251 cells stained with phospho-ULK1 antibody at 1:200. Immunoreactivity is localized to autophagic vacuoles in the cytoplasm.
Phospho-ULK1 Antibody (pS556)
Target: ULK1
Antibodies, Primary Antibodies, Phospho Antibodies Immunofluorescence, Dot Blot, ELISA
From $205.00
From $205.00
In Stock at Manufacturer
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Dot blot analysis of phospho Beclin-1 antibody. 50ng of phos-peptide or nonphos-peptide per dot were spotted.
Phospho-Beclin-1 Antibody (pT119)
Target: Beclin-1
Antibodies, Primary Antibodies, Phospho Antibodies Dot Blot, ELISA
From $205.00
From $205.00
In Stock at Manufacturer
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Western blot testing of p62 antibody at 1:500 dilution + HeLa lysate; Predicted band size : 47~62 kDa.
p62 Antibody
Target: p62
Antibodies, Primary Antibodies Western Blot, Immunofluorescence, Immunohistochemistry, ELISA
From $205.00
From $205.00
In Stock at Manufacturer
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SQSTM1 antibody western blot analysis in HeLa lysate. Observed molecular weight ~62 kDa.
SQSTM1 Antibody
Target: SQSTM1
Antibodies, Primary Antibodies Western Blot, ELISA
From $205.00
From $205.00
In Stock at Manufacturer
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ULK1 antibody western blot analysis in U251 lysate. Predicted molecular weight: 112-150 kDa
ULK1 Antibody
Target: ULK1
Antibodies, Primary Antibodies Western Blot, ELISA
From $205.00
From $205.00
In Stock at Manufacturer
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ULK1 antibody western blot analysis in HeLa lysate. Predicted molecular weight: 112-150 kDa.
ULK1 Antibody
Target: ULK1
Antibodies, Primary Antibodies Western Blot, ELISA
From $205.00
From $205.00
In Stock at Manufacturer
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