Autophagy & Flux Antibodies

Validated antibodies for autophagy and autophagic-flux studies — LC3B (LC3-I to LC3-II conversion), p62/SQSTM1, Beclin-1, ULK1, ATG5, ATG7 and more, for Western blot, IHC, and IF. Read LC3-II and p62 together, with the right controls (± lysosomal inhibitor), so a band change reflects real flux, not the antibody.

212Products
212 products
Image ProductProduct Type ApplicationPrice Status ACTION
TFEB antibody western blot analysis in HepG2 lysate. Expected molecular weight: 53-60 kDa (unmodified), 65-70 kDa (modified).
Target: TFEB
Antibodies, Primary Antibodies Western Blot, ELISA
From $205.00
Regular price From $205.00
In Stock at Manufacturer
Fluorescent image of chloroquine-treated U251 cells stained with ATG12 antibody. Alexa Fluor 488 conjugated secondary was used. ATG12 immunoreactivity is localized to autophagic vacuoles in the cytoplasm.
Target: ATG12
Antibodies, Primary Antibodies Immunofluorescence, Western Blot, Immunohistochemistry, ELISA
From $205.00
Regular price From $205.00
In Stock at Manufacturer
Western blot analysis of lysate from HeLa, HT1080 and human placenta lysate (left to right), using LAMP1 antibody at 1:1000. This heavily glycosylated protein of 417 amino acids is visualized at up to ~140 kDa.
Target: LAMP1
Antibodies, Primary Antibodies Immunohistochemistry, Western Blot, ELISA
From $205.00
Regular price From $205.00
In Stock at Manufacturer
Western blot analysis of anti-LAMP2 antibody and T47D lysate. The protein can be extensively glycosylated and visualized from 45~110 kDa.
Target: LAMP2
Antibodies, Primary Antibodies Western Blot, ELISA
From $205.00
Regular price From $205.00
In Stock at Manufacturer
Fluorescent image of U251 cells stained with anti-ATG5 antibody diluted at 1:25 dilution. U251 cells were treated with Chloroquine (50 uM, 16h), An Alexa Fluor 488-conjugated goat anti-rabbit lgG was used as the secondary Ab (green). DAPI was used as a nuclear counterstain (blue).
Target: ATG5
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
From $205.00
Regular price From $205.00
In Stock at Manufacturer
ATG7 antibody western blot analysis in Jurkat lysate. Predicted molecular weight: 70-80 kDa.
Target: ATG7
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, ELISA
From $205.00
Regular price From $205.00
In Stock at Manufacturer
Fluorescent image of Chloroquine treated U251 cells stained with ATG7 antibody at 1:200. Immunoreactivity is localized to autophagic vacuoles in the cytoplasm.
Target: ATG7
Antibodies, Primary Antibodies Immunofluorescence, Western Blot, Immunohistochemistry, ELISA
From $205.00
Regular price From $205.00
In Stock at Manufacturer
Western blot testing of Beclin antibody and (1) A2058, (2) HeLa, (3) mouse brain, (4) Y79, and (5) HL-60 lysate
Target: Beclin
Antibodies, Primary Antibodies Flow Cytometry, Western Blot, Immunohistochemistry, ELISA
From $205.00
Regular price From $205.00
In Stock at Manufacturer
Western blot analysis of ATG5 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (2) with the human gene. Predicted molecular weight ATG5: ~32 kDa; ATG5/ATG12 heterodimer: ~56 kDa.
Target: ATG5
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
From $205.00
Regular price From $205.00
In Stock at Manufacturer
Western blot analysis of Beclin 1 antibody and 293 lysate transiently transfected with the BECN1 gene (2ug/lane).
Target: BECN1
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, ELISA
From $205.00
Regular price From $205.00
In Stock at Manufacturer
Western blot analysis of ATG12 antibody and MCF-7 lysate.
Target: ATG12
Antibodies, Primary Antibodies Western Blot, Immunofluorescence, ELISA
From $205.00
Regular price From $205.00
In Stock at Manufacturer
LAMP2 antibody western blot analysis in mouse NIH3T3 lysate. The protein can be extensively glycosylated and visualized from 45~110 kDa.
Target: LAMP2
Antibodies, Primary Antibodies Western Blot, Immunofluorescence, ELISA
From $205.00
Regular price From $205.00
In Stock at Manufacturer
Western blot analysis of ATG12 antibody and recombinant protein.
Target: ATG12
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
From $205.00
Regular price From $205.00
In Stock at Manufacturer
Rabbit Anti-Beclin 1 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on SK-N-BE (SPC-601)
Target: Beclin 1
Antibodies, Primary Antibodies Western Blot, Immunofluorescence
From $338.00
Regular price From $338.00
In Stock at Manufacturer
Rabbit Anti-ATG7 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Colon carcinoma cell line (RKO) (SPC-610)
Target: ATG7
Antibodies, Primary Antibodies Western Blot, Immunofluorescence, Immunohistochemistry
From $338.00
Regular price From $338.00
In Stock at Manufacturer
Rabbit Anti-Beclin 1 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Neuroblastoma cell line (SK-N-BE) (SPC-600)
Target: Beclin 1
Antibodies, Primary Antibodies Western Blot, Immunofluorescence
From $338.00
Regular price From $338.00
In Stock at Manufacturer
Rabbit Anti-ULK1 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Neuroblastoma cell line (SK-N-BE) (SPC-640)
Target: ULK1
Antibodies, Primary Antibodies Western Blot, Immunofluorescence
From $338.00
Regular price From $338.00
In Stock at Manufacturer
Mouse Anti-LAMP1 Antibody [Ly1C6] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human transfected HeLa cells (SMC-140)
Target: LAMP1
Antibodies, Primary Antibodies Western Blot, Immunofluorescence, Immunoprecipitation
From $326.00
Regular price From $326.00
In Stock at Manufacturer
Rat Anti-LAMP2 Antibody [GL2A7] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Rabbit Corneal Endothelial Cell (CEC) (SMC-141)
Target: LAMP2
Antibodies, Primary Antibodies Western Blot, Immunofluorescence, Immunoprecipitation
From $313.00
Regular price From $313.00
In Stock at Manufacturer
Anti-Human CD107a/LAMP1 Antibody (Mab1), PE
Target: CD107a
Antibodies, Primary Antibodies, Flow Cytometry Antibodies Flow Cytometry
$352.00
Regular price $352.00
In Stock at Manufacturer
Anti-Human CD107a/LAMP1 Antibody (Mab1)
Target: CD107a
Antibodies, Primary Antibodies, Flow Cytometry Antibodies Flow Cytometry
$270.00
Regular price $270.00
In Stock at Manufacturer
Western blot analysis was performed using anti-ULK1 polyclonal antibody at 1ug/mL  on various samples.
Lane 1: Mouse brain lysate
Target: ULK1
Antibodies, Primary Antibodies ELISA, Immunohistochemistry, Western Blot
From $168.00
Regular price From $168.00
In Stock at Manufacturer
Western blot analysis was performed using anti-Human ATG10 polyclonal antibody at 1ug/mL on recombinant Human ATG10 (Catalog No. HB377012).
Target: ATG10
Antibodies, Primary Antibodies ELISA, Immunohistochemistry, Western Blot
From $168.00
Regular price From $168.00
In Stock at Manufacturer
Anti-Human CD107a/LAMP1 Antibody (Mab1), FITC
Target: CD107a
Antibodies, Primary Antibodies, Flow Cytometry Antibodies Flow Cytometry
$270.00
Regular price $270.00
In Stock at Manufacturer
Flow-cytometry using APC anti-human CD107a antibody. Hela cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD107a monoclonal antibody (Catalog: HY332137, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Target: CD107a
Antibodies, Primary Antibodies, Flow Cytometry Antibodies Flow Cytometry
$352.00
Regular price $352.00
In Stock at Manufacturer
SDS-PAGE for Anti-Beclin-1 Antibody (N248A/15)
Target: Beclin-1
Antibodies, Primary Antibodies, Recombinant Antibodies Immunohistochemistry, Western Blot
From $196.00
Regular price From $196.00
In Stock at Manufacturer
SDS-PAGE for Anti-Beclin-1 Antibody (N248A/22)
Target: Beclin-1
Antibodies, Primary Antibodies, Recombinant Antibodies Immunohistochemistry, Western Blot
From $196.00
Regular price From $196.00
In Stock at Manufacturer
SDS-PAGE for Anti-Beclin-1 Antibody (N248/32)
Target: Beclin-1
Antibodies, Primary Antibodies, Recombinant Antibodies Immunohistochemistry, Western Blot
From $196.00
Regular price From $196.00
In Stock at Manufacturer
Western blot analysis was performed using anti-CD107b/LAMP2 polyclonal antibody at 1ug/mL on recombinant CD107b/LAMP2 (Catalog No. HY293012).
Target: LAMP2
Antibodies, Primary Antibodies ELISA, Immunohistochemistry, Western Blot
From $168.00
Regular price From $168.00
In Stock at Manufacturer
Western blot analysis was performed using anti-SQSTM1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: K562 cell lysate
Target: SQSTM1
Antibodies, Primary Antibodies ELISA, Immunohistochemistry, Western Blot
From $168.00
Regular price From $168.00
In Stock at Manufacturer
Western blot analysis was performed using anti-ATG5 polyclonal antibody at 1ug/mL on recombinant ATG5 (Catalog No. HV332012).
Target: ATG5
Antibodies, Primary Antibodies ELISA, Immunohistochemistry, Western Blot
From $168.00
Regular price From $168.00
In Stock at Manufacturer
Western blot analysis was performed using anti-Human ATG7 polyclonal antibody at 1ug/mL on recombinant Human ATG7 (Catalog No. HF840012).
Target: ATG7
Antibodies, Primary Antibodies ELISA, Immunohistochemistry, Western Blot
From $168.00
Regular price From $168.00
In Stock at Manufacturer

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