Denarase®
Endonuclease |
You need to degrade host-cell DNA and RNA to reduce viscosity and meet residual-DNA limits, and your process stream is at or below about 150 mM NaCl. |
Purity ≥99% (SDS-PAGE, silver staining)
Activity >250 U/µL
Endotoxin <0.25 EU/kU
−20 °C ± 5 °C, at least 36 months
Mg²⁺ optimum 1–2 mM, pH optimum 8.0–9.0 |
R&D: 100, 500, 1,000 and 5,000 kU
GMP: 1 MU and 5 MU |
Denarase® High Salt
Salt-tolerant endonuclease |
Your stream runs above 150 mM NaCl — for example after elution or in high-conductivity harvest — where standard nuclease activity falls away. Between 150 and 250 mM, test both enzymes. |
Purity ≥98% (SDS-PAGE, silver staining)
Activity >250 U/µL across 0–500 mM NaCl
Endotoxin <0.25 EU/kU
−20 °C ± 5 °C, at least 30 months
Mg²⁺ 5 mM standard, rising to 25 mM; pH optimum 7.4–9.0 |
R&D: 25, 100, 500, 1,000 and 5,000 kU
GMP: 1 MU and 5 MU |
Denarase® ELISA Kit
Residual-nuclease assay |
You have to show how much Serratia marcescens endonuclease is left in the product. One kit covers Denarase®, Denarase® High Salt and Benzonase®. |
Limit of detection 4 pg/mL
Limit of quantification 12 pg/mL
Working range approx. 32–1,000 pg/mL
480 measurements (5 × 96-well plates)
About 130 minutes total incubation
2–8 °C, 18 months |
Complete kit, or replacement wash and dilution buffer (500 mL, 10× concentrate)
For research and manufacturing use; not for diagnostic procedures |
CellTrypase
Cell dissociation enzyme |
You are detaching adherent cells and need an animal origin-free enzyme with GMP documentation — viral vector and vaccine production, cell banking, or passaging sensitive cell types. |
Purity ≥95% (size-exclusion HPLC)
1× 0.6–1.2 kU/L; 10× 6–12 kU/L
Endotoxin ≤1 EU/mL (1×), ≤10 EU/mL (10×)
Mycoplasma negative, sterility pass
2–8 °C, at least 21 months; ships at ambient temperature |
R&D and GMP, each as 1× or 10×, in 100 mL and 500 mL
Animal origin-free; no inhibitor needed to stop the reaction |
