KO-Validated Antibodies

Primary antibodies validated in knockout (KO) models, where the signal disappears in the knockout sample — the strongest orthogonal evidence that an antibody binds only its intended target.

22Products
22 products
Image ProductProduct Type ApplicationPrice Status ACTION
Western blot analysis of extracts of various cell lines, using TLK2 antibody  at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG   at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit .
Exposure time: 90s.
Primary Antibody Western Blot
From $258.00
Regular price From $258.00
In Stock at Manufacturer
Western blot analysis of extracts of various cell lines, using NF2 antibody  at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG   at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 60s.
Primary Antibody Western Blot, Immunofluorescence, Immunoprecipitation
From $258.00
Regular price From $258.00
In Stock at Manufacturer
Western blot analysis of extracts of various cell lines, using GARS antibody  at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG   at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 60s.
Primary Antibody Western Blot, Immunohistochemistry, Immunofluorescence
From $258.00
Regular price From $258.00
In Stock at Manufacturer
Western blot analysis of extracts from wild type and MTCH2 knockout  HeLa cells, using MTCH2 antibody  at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG   at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 3s.
Primary Antibody Western Blot, Immunohistochemistry, Immunofluorescence
From $258.00
Regular price From $258.00
In Stock at Manufacturer
[KO Validated] SUZ12 polyclonal antibody – Figure 1 of 5
Primary Antibody Western Blot, Immunofluorescence, Immunoprecipitation
From $258.00
Regular price From $258.00
In Stock at Manufacturer
[KO Validated] SETD7 polyclonal antibody – Figure 1 of 1
Primary Antibody Western Blot, Immunofluorescence
From $258.00
Regular price From $258.00
In Stock at Manufacturer
Western blot analysis of extracts of Rat liver, using AMPKα2 antibody  at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG   at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 180s.
Primary Antibody Western Blot, Immunofluorescence
From $258.00
Regular price From $258.00
In Stock at Manufacturer
[KO Validated] RhoC polyclonal antibody – Figure 1 of 3
Primary Antibody Western Blot, Immunohistochemistry, Immunofluorescence
From $258.00
Regular price From $258.00
In Stock at Manufacturer
Western blot analysis of extracts of various cell lines, using CD73/NT5E antibody  at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG   at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.
Primary Antibody Western Blot, Immunohistochemistry, Immunofluorescence
From $258.00
Regular price From $258.00
In Stock at Manufacturer
Western blot analysis of extracts from normal  and KMT2A knockout  293T cells, using KMT2A antibody  at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG   at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 180s.
Primary Antibody Western Blot, Immunohistochemistry
From $258.00
Regular price From $258.00
In Stock at Manufacturer
Western blot analysis of extracts of various cell lines, using EEF2K antibody  at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG   at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 40s.
Polyclonal Antibody Western Blot, Immunofluorescence
From $258.00
Regular price From $258.00
In Stock at Manufacturer
Western blot analysis of extracts of various cell lines, using HIF1AN/FIH1 antibody  at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG   at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.
Polyclonal Antibody Western Blot, Immunofluorescence, Immunoprecipitation
From $258.00
Regular price From $258.00
In Stock at Manufacturer
Western blot analysis of extracts of various cell lines, using BAF57/BAF57/SMARCE1 antibody  at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG   at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 90s.
Polyclonal Antibody Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
From $258.00
Regular price From $258.00
In Stock at Manufacturer
[KO Validated] SUCLG2 polyclonal antibody – Figure 1 of 5
Polyclonal Antibody Western Blot, Immunohistochemistry, Immunofluorescence
From $258.00
Regular price From $258.00
In Stock at Manufacturer
Western blot analysis of extracts from wild type and GCN1L1 knockout  293T cells, using GCN1L1 antibody  at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG   at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.
Polyclonal Antibody Western Blot
From $258.00
Regular price From $258.00
In Stock at Manufacturer
[KO Validated] DDIT4 polyclonal antibody – Figure 1 of 2
Polyclonal Antibody Western Blot
From $258.00
Regular price From $258.00
In Stock at Manufacturer
[KO Validated] PRMT6 polyclonal antibody – Figure 1 of 2
Polyclonal Antibody Western Blot, Immunofluorescence
From $258.00
Regular price From $258.00
In Stock at Manufacturer
Western blot analysis of extracts of various cell lines, using XIAP antibody  at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG   at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.
Polyclonal Antibody Western Blot
From $258.00
Regular price From $258.00
In Stock at Manufacturer
Western blot analysis of extracts from normal  and IGF1R knockout  293T cells, using IGF1R antibody  at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG   at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 10s.
Polyclonal Antibody Western Blot
From $258.00
Regular price From $258.00
In Stock at Manufacturer
Western blot analysis of extracts from normal  and RBBP4 knockout  293T cells, using RBBP4 antibody  at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG   at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.
Polyclonal Antibody Western Blot, Immunofluorescence, ChIP
From $258.00
Regular price From $258.00
In Stock at Manufacturer
Western blot analysis of extracts of various cell lines, using HDAC1 antibody  at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG   at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 60s.
Polyclonal Antibody Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
From $258.00
Regular price From $258.00
In Stock at Manufacturer
Western blot analysis of extracts of various cell lines, using APEX1/APE1 antibody .
Secondary antibody: HRP Goat Anti-Rabbit IgG   at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Polyclonal Antibody Western Blot, Immunofluorescence, Immunoprecipitation
From $258.00
Regular price From $258.00
In Stock at Manufacturer

About KO-Validated Antibodies

Antibody specificity is a leading cause of irreproducible results. Knockout (KO) validation is the gold-standard control: when the target gene is knocked out, a specific antibody's signal disappears. Every product in this collection carries KO-validation data on its page.

How antibody validation levels compare

Validation methodWhat it showsConfidence
Knockout (KO)Signal is lost in KO sample — antibody is specific to the targetHighest (orthogenal genetic control)
Knockdown (siRNA/shRNA)Signal is reduced when target is silencedHigh, but residual expression remains
OverexpressionSignal increases with target overexpressionSupportive; does not rule out off-target bands
Orthogonal / IP-MSIndependent method confirms identityHigh; method-dependent

Choose KO-validated antibodies when you need the strongest evidence of specificity — for example, before publishing Western blot or IHC data.

Recommended KO-validated antibodies

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