Protein Quality Control — Antibodies

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IHC staining of FFPE human testis tissue with HSP90 beta antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
HSP90 beta Antibody / HSP90AB1
Target: HSP90 BETA
Antibodies, Primary Antibodies Immunohistochemistry, Western Blot
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Western blot testing of human 1) HeLa and 2) Raji cell lysate with HSP90AB1 antibody. Predicted molecular weight: 84-90 kDa.
HSP90AB1 Antibody / HSP90 beta
Target: HSP90AB1
Antibodies, Primary Antibodies Immunohistochemistry, Western Blot
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Western blot testing of human HeLa cell lysate with HSP90AA1 antibody. Predicted molecular weight: 86-90 kDa.
HSP90AA1 Antibody / HSP90 alpha
Target: HSP90AA1
Antibodies, Primary Antibodies Immunohistochemistry, Western Blot
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IHC staining of FFPE human breast cancer tissue with HSP90 alpha/beta antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
HSP90 alpha/beta Antibody
Target: HSP90 ALPHA/BETA
Antibodies, Primary Antibodies Immunohistochemistry, Western Blot, Immunofluorescence
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Western blot testing of human Jurkat cell lysate with HSP90AA1 antibody. Predicted molecular weight: 86-90 kDa.
HSP90AA1 Antibody / HSP90 alpha
Target: HSP90AA1
Antibodies, Primary Antibodies Western Blot
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Western blot testing of 1) rat PC-12 and 2) human HeLa cell lysate with HSP90 alpha antibody. Predicted molecular weight: 86-90 kDa.
HSP90 alpha Antibody / HSP90AA1
Target: HSP90 ALPHA
Antibodies, Primary Antibodies Western Blot
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Western blot testing of lysate from human A431 cells 1) untreated or 2) treated with Anisomycin, with phospho-HSP27 antibody (pS78). Expected molecular weight: 24-27 kDa.
Phospho-HSP27 Antibody (pS78)
Target: HSP27
Antibodies, Primary Antibodies Western Blot
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Western blot testing of human HeLa cell lysate with HSP27 antibody. Expected molecular weight: 23-27 kDa.
HSP27 Antibody
Target: HSP27
Antibodies, Primary Antibodies Western Blot
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Western blot testing of human serum lysate with von Willebrand Factor antibody. Predicted molecular weight ~309 kDa.
von Willebrand Factor Antibody / VWF
Target: VON WILLEBRAND FACTOR
Antibodies, Primary Antibodies Western Blot
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Western blot testing of human serum lysate with Transferrin antibody. Predicted molecular weight ~77 kDa.
Transferrin Antibody
Target: TRANSFERRIN
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
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Western blot testing of human fetal heart lysate with Alpha B Crystallin antibody. Predicted molecular weight ~20 kDa.
Alpha B Crystallin Antibody / CRYAB
Target: ALPHA B CRYSTALLIN
Antibodies, Primary Antibodies Western Blot
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Western blot testing of human 1) HepG2 and 2) PC-3 lysate with TMEM166 antibody at 0.5ug/ml. Predicted molecular weight ~17 kDa.
TMEM166 Antibody / EVA1A
Target: TMEM166
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Flow Cytometry
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Western blot testing of 1) human Caco-2, 2) rat brain and 3) mouse brain lysate with HECTD3 antibody at 0.5ug/ml. Predicted molecular weight ~97 kDa.
HECTD3 Antibody
Target: HECTD3
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Flow Cytometry
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Western blot testing of 1) human HepG2, 2) mouse spleen and 3) rat kidney lysate with ATG7 antibody. Predicted molecular weight: 70-80 kDa.
ATG7 Antibody
Target: ATG7
Antibodies, Primary Antibodies Western Blot
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Western blot testing of human HeLa cell lysate with BAG2 antibody. Predicted molecular weight ~22 kDa.
BAG2 Antibody
Target: BAG2
Antibodies, Primary Antibodies Western Blot
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Western blot testing of human HepG2 cell lysate with ATG7 antibody. Predicted molecular weight: 70-80 kDa.
ATG7 Antibody
Target: ATG7
Antibodies, Primary Antibodies Western Blot
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Western blot testing of human HepG2 lysate with ATG12 antibody. Expected molecular weight: ~15 kDa (ATG12) and 50-60 kDa (ATG12-ATG5 complex).
ATG12 Antibody
Target: ATG12
Antibodies, Primary Antibodies Western Blot
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Western blot testing of human K562 cell lysate with BAG3 antibody. Predicted molecular weight ~62 kDa but can be observed at 75~80 kDa.
BAG3 Antibody
Target: BAG3
Antibodies, Primary Antibodies Western Blot
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Western blot testing of lysate from 1) untreated and 2) AP-treated human K562 cells with phospho-IRE1 antibody. Predicted molecular weight ~110 kDa.
Phospho-IRE1 Antibody (pS724)
Target: IRE1
Antibodies, Primary Antibodies Western Blot
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Western blot testing of 1) human HEK293 and 2) rat PC-12 cell lysate with ULK1 antibody. Predicted molecular weight: 112-150 kDa.
ULK1 Antibody
Target: ULK1
Antibodies, Primary Antibodies Western Blot
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Western blot testing of human Jurkat cell lysate with PARK2 antibody. Expected molecular weight: 50-60 kDa with multiple smaller isoforms (possible ~30 kDa isoform visualized here).
PARK2 Antibody / Parkin
Target: PARK2
Antibodies, Primary Antibodies Western Blot
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Western blot testing of human U-87 MG cell lysate with HSPA12A antibody. Expected molecular weight ~70 kDa.
HSPA12A Antibody / Heat shock 70 kDa protein 12A
Target: HSPA12A
Antibodies, Primary Antibodies Immunohistochemistry, Western Blot
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Western blot testing of mouse skeletal muscle lysate with Hsp20 antibody. Predicted molecular weight: 17-20 kDa.
Hsp20 Antibody / HSPB6
Target: HSP20
Antibodies, Primary Antibodies Western Blot
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Western blot testing of human HepG2 cell lysate with Ubiquitin D antibody. Expected molecular weight: 18-22 kDa.
Ubiquitin D Antibody / UBD / FAT10
Target: UBIQUITIN D
Antibodies, Primary Antibodies Western Blot
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Western blot testing of human 1) U-87 MG, 2) HepG2 and 3) A375 cell lysate with Mib1 antibody at 0.5ug/ml. Predicted molecular weight ~110 kDa.
Mib1 Antibody / Mindbomb
Target: MIB1
Antibodies, Primary Antibodies Western Blot, ELISA
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Western blot testing of 1) rat skeletal muscle, 2) rat lung and mouse 3) spleen, 4) skeletal muscle, 5) lung, 6) liver and 7) testis lysate with Hsp27 antibody at 0.5ug/ml. Predicted molecular weight: 23-27 kDa.
Hsp27 Antibody
Target: HSP27
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, ELISA
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IHC staining of FFPE human esophagus squamous cancer with AHR antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
AHR Antibody / Aryl Hydrocarbon Receptor
Target: AHR
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Flow Cytometry
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Western blot testing of 1) human K562, 2) rat brain and 3) mouse brain lysate with CUL2 antibody at 0.5ug/ml. Predicted molecular weight: ~87 kDa.
CUL2 Antibody / Cullin 2
Target: CUL2
Antibodies, Primary Antibodies Western Blot, Flow Cytometry, ELISA
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Western blot testing of human 1) HeLa, 2) COLO-320, 3) SW620, 4) A431, 5) A549, 6) HepG2 and 7) PANC-1 lysate with HSP70 antibody at 0.5ug/ml. Expected molecular weight ~70 kDa.
HSP70 Antibody / HSPA1A / HSPA1B
Target: HSP70
Antibodies, Primary Antibodies Flow Cytometry, Immunohistochemistry, Western Blot, Immunofluorescence
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Western blot testing of human 1) HeLa, 2) placenta, 3) COLO-320, 4) SGC-7901, 5) HepG2, 6) K562, 7) Jurkat and 8) SK-OV-3 lysate with HSP105 antibody at 0.5ug/ml. Expected molecular weight: 105-110 kDa.
HSP105 Antibody
Target: HSP105
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, ELISA
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Western blot testing of human 1) HeLa, 2) MBA-MD-231, 3) COLO-320, 4) PANC-1, 5) HT180, 6) MBA-MD-453 and 7) HepG2 lysate with HSPA2 antibody at 0.5ug/ml. Expected molecular weight ~70 kDa.
HSPA2 Antibody
Target: HSPA2
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
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Western blot testing of human 1) HeLa, 2) 293T, 3) COLO-320, 4) SW620 and 5) MCF7 cell lysate with RNF43 antibody at 0.5ug/ml. Predicted molecular weight: ~86 kDa (isoform 1), ~95 kDa (isoform 4).
RNF43 Antibody
Target: RNF43
Antibodies, Primary Antibodies Western Blot, ELISA
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Western blot testing of human 1) U-87 MG and 2) MDA-MB-453 cell lysate with NEDD4 antibody at 0.5ug/ml. Predicted molecular weight ~149 kDa; commonly observed at 110-149 kDa with a possible ~95 kDa cleavage band.
NEDD4 Antibody
Target: NEDD4
Antibodies, Primary Antibodies Western Blot, ELISA
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Western blot testing of rat 1) brain, 2) spleen, 3) heart, 4) kidney and mouse 5) brain, 6) spleen, 7) heart, 8) kidney and 9) testis lysate with Cullin 5 antibody at 0.5ug/ml. Predicted molecular weight ~91 kDa.
Cullin 5 Antibody / CUL5
Target: CULLIN 5
Antibodies, Primary Antibodies Western Blot, ELISA
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Western blot testing of human 1) HeLa and 2) HepG2 cell lysate with CLPX antibody at 0.5ug/ml. Predicted molecular weight ~69 kDa.
CLPX Antibody / Caseinolytic protease X
Target: CLPX
Antibodies, Primary Antibodies Western Blot, Flow Cytometry, Immunohistochemistry, Immunofluorescence, ELISA
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Western blot testing of human 1) HeLa 2) placenta, 3) MCF7, 4) A549, 5) SK-OV-3, 6) 22RV1, 7) A431 and 8) COLO320 lysate with UBA2 antibody at 0.5ug/ml. Predicted molecular weight: ~72 kDa but routinely observed at ~90 kDa.
UBA2 Antibody / SUMO-activating enzyme subunit 2 / SAE2
Target: UBA2
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, ELISA
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