Validated Cancer Antibodies

Application-validated cancer-research antibodies, each validated for two or more applications spanning Western blot, immunohistochemistry and immunofluorescence. Check each datasheet for the applications validated for your target. Save 15% with code ONCO15.

4395Products
4395 products
Image ProductProduct Type ApplicationPrice Status ACTION
Western Blot
Positive WB detected in: HeLa whole cell lysate(30µg) , A549 whole cell lysate(30µg) , MCF-7 whole cell lysate(30µg) , U-251MG whole cell lysate(30µg) , SH-SY5Y whole cell lysate(30µg) , THP-1 whole cell lysate(30µg) , HT-29 whole cell lysate(30µg) , Mouse brain tissue lysate(30µg) , Rat heart tissue lysate(30µg) 
All lanes: VEGFD antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 40 kDa
Observed band size: 40 kDa
Exposure time:2min
Target: VEGFD
Antibodies, Primary Antibodies, Recombinant Antibodies ELISA, Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
From $216.30
Regular price From $216.30
In Stock at Manufacturer
Western blot testing with VEGFR2 antibody and SMMC-7721 lysate. Predicted molecular weight: ~152 (immature), 180-200 kDa (intermediate) and 220-230 kDa (mature).
Target: VEGFR2
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Immunofluorescence
$462.47
Regular price $462.47
In Stock at Manufacturer
Western blot analysis of VEGFR2 (pY1214) expression in HuvEc (A), HepG2 (B) whole cell lysates.
Polyclonal Antibody Western Blot, Immunohistochemistry, Immunofluorescence
From $173.04
Regular price From $173.04
In Stock at Manufacturer
Western blot testing of human 1) HeLa, 2) MCF7 and 3) HepG2 cell lysate with VEGFR3 antibody at 0.5ug/ml. Predicted molecular weight ~153 kDa (long), ~147 kDa (short) and ~93 kDa (sVegfr3).
Target: VEGFR3
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
$462.47
Regular price $462.47
In Stock at Manufacturer
VHL antibody IHC analysis in formalin fixed and paraffin embedded mouse brain tissue.
Target: VHL
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, ELISA
From $211.15
Regular price From $211.15
In Stock at Manufacturer
VIL1 antibody – Figure 1 of 3
Target: VIL1
Primary Antibody ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
$338.00
Regular price $338.00
In Stock at Manufacturer
Western blot
All lanes: VIM antibody at 8µg/ml
Lane 1: Hela whole cell lysate
Lane 2: Jurkat whole cell lysate
Lane 3: A549 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa
Target: VIM
Antibodies, Primary Antibodies ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
From $170.98
Regular price From $170.98
In Stock at Manufacturer
Western Blot analysis of 293 cells using Vimentin Polyclonal Antibody
Target: VIM
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
From $122.57
Regular price From $122.57
In Stock at Manufacturer
Western Blot analysis of HepG2 cells using VIM Polyclonal Antibody
Target: VIM
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
From $122.57
Regular price From $122.57
In Stock at Manufacturer
Western Blot analysis of A549 cells using Vimentin Polyclonal Antibody
Target: VIM
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
From $122.57
Regular price From $122.57
In Stock at Manufacturer
VIM Antibody – Figure 1 of 1
Target: VIM
Antibodies, Primary Antibodies ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
$618.00
Regular price $618.00
In Stock at Manufacturer
IHC staining of FFPE human colon tissue with VIM antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Target: VIM
Antibodies, Primary Antibodies Immunohistochemistry, Western Blot, Immunofluorescence
$462.47
Regular price $462.47
In Stock at Manufacturer
Immunofluorescent staining of FFPE human intestinal cancer with Vimentin antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA for 20 min.
Target: VIM
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Immunofluorescence
$462.47
Regular price $462.47
In Stock at Manufacturer
IHC-P: Vimentin antibody testing of human breast cancer tissue. Required HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Target: VIM
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Immunofluorescence
$462.47
Regular price $462.47
In Stock at Manufacturer
IHC staining of FFPE human rectal cancer with VPRBP antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Target: DCAF1
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, ELISA
$462.47
Regular price $462.47
In Stock at Manufacturer
IHC staining of human tonsil with vWF antibody (clone 3E2D10). Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
Target: VWF
Antibodies, Primary Antibodies Flow Cytometry, Immunofluorescence, Western Blot, Immunoprecipitation, Immunohistochemistry
From $266.77
Regular price From $266.77
In Stock at Manufacturer
IHC staining of FFPE human stomach cancer tissue with WAPL antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Target: WAPL
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, ELISA
$462.47
Regular price $462.47
In Stock at Manufacturer
Western blot testing of rat 1) heart, 2) kidney, 3) lung and human 4) A549, 5) A431 and 6) COLO320 lysate with WISP1 antibody. Expected molecular weight: 40-55 kDa depending on degree of glycosylation.
Target: WISP1
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Immunofluorescence
$462.47
Regular price $462.47
In Stock at Manufacturer
WNT1 Antibody – Figure 1 of 2
Target: WNT1
Primary Antibody Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
From $122.57
Regular price From $122.57
In Stock at Manufacturer
Western blot analysis of extracts from HT-29 cells, using WNT1 antibody.
Target: WNT1
Antibodies, Primary Antibodies ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
$305.91
Regular price $305.91
In Stock at Manufacturer
WT1 antibody western blot analysis in MCF-7 lysate. Predicted molecular weight: ~49 kDa
Target: WT1
Antibodies, Primary Antibodies Immunofluorescence, Flow Cytometry, Western Blot, Immunohistochemistry, ELISA
From $211.15
Regular price From $211.15
In Stock at Manufacturer
IHC staining of FFPE human ovarian cancer tissue with X-ray repair cross-complementing 4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Target: XRCC4
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, ELISA
$462.47
Regular price $462.47
In Stock at Manufacturer
Western Blot analysis of HepG2 cells using Phospho-XIAP (S87) Polyclonal Antibody diluted at 1:500
Primary Antibody Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
From $192.40
Regular price From $192.40
In Stock at Manufacturer
XIAP antibody – Figure 1 of 4
Target: XIAP
Primary Antibody ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
$312.00
Regular price $312.00
In Stock at Manufacturer
Immunofluorescent analysis of A549 cells using XRCC1 antibody at 1:25. Alexa Fluor 488-conjugated secondary was used (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 conjugated Phalloidin (red).
Target: XRCC1
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Flow Cytometry, Immunofluorescence, ELISA
From $211.15
Regular price From $211.15
In Stock at Manufacturer
Western blot testing of XRCC1 antibody and Lane 1: 293T; 2: A431; 3: HeLa cell lysate. Rountinely observed molecular weight: 69~90 kDa.
Target: XRCC1
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Immunofluorescence
$462.47
Regular price $462.47
In Stock at Manufacturer
Western blot testing of human 1) HeLa, 2) placenta, 3) MCF7, 4) HepG2, 5) A549, 6) SKOV3, 7) PANC-1 and 8) mouse testis and 9) rat testis lysate with XRCC1 antibody at 0.5ug/ml. Rountinely observed molecular weight: 69~90 kDa.
Target: XRCC1
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
$462.47
Regular price $462.47
In Stock at Manufacturer
Western blot (WB) analysis of YAP (Q295) pAb at 1:500 dilution
Lane1:H9C2 whole cell lysate(40ug)
Lane2:MEF whole cell lysate(40ug)
Lane3:L02 whole cell lysate(40ug)
Lane4:PC3 whole cell lysate(40ug)
Polyclonal Antibody Western Blot, Immunohistochemistry, Immunofluorescence
From $162.74
Regular price From $162.74
In Stock at Manufacturer
Immunofluorescent staining of FFPE human A549 cells with YAP1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Target: YAP1
Antibodies, Primary Antibodies Western Blot, Immunofluorescence, Flow Cytometry, Immunoprecipitation, Immunohistochemistry
$462.47
Regular price $462.47
In Stock at Manufacturer
Immunofluorescent staining of FFPE human A431 cells with YB1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Target: YB1
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
$462.47
Regular price $462.47
In Stock at Manufacturer
Immunofluorescent staining of FFPE human HepG2 cells with YTHDF2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Target: YTHDF2
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Flow Cytometry, Immunofluorescence
$462.47
Regular price $462.47
In Stock at Manufacturer
IHC staining of FFPE human laryngeal squamous cell carcinoma tissue with YWHAE antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Target: 14-3-3 Epsilon/YWHAE
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
$462.47
Regular price $462.47
In Stock at Manufacturer
IHC staining of FFPE human prostate cancer tissue with YWHAE antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Target: 14-3-3 Epsilon/YWHAE
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
$462.47
Regular price $462.47
In Stock at Manufacturer
Immunofluorescent staining of PFA-fixed human Jurkat cells with ZAP-70 antibody (green, clone 2F3.2) and Reddot nuclear stain (red).
Target: ZAP-70
Antibodies, Primary Antibodies Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry
From $266.77
Regular price From $266.77
In Stock at Manufacturer
Western blot (WB) analysis of ZBP-89 (E84) at 1:500 dilution
Lane1:H9C2 whole cell lysate(40ug)
Lane2:SP2/0 whole cell lysate(40ug)
Lane3:Hela whole cell lysate(40ug)
Lane4:HEK293T whole cell lysate(40ug)
Lane5:U-87MG whole cell lysate(40ug)
Polyclonal Antibody Western Blot, Immunohistochemistry, Immunofluorescence
From $162.74
Regular price From $162.74
In Stock at Manufacturer
Western blot testing of human 1) SGC-7901, 2) U-87 MG, 3) HEK293 and 4) PC-3 cell lysate with ZEB1 antibody. Predicted molecular weight ~124 kDa but observed at up to ~200 kDa.
Target: ZEB1
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
$462.47
Regular price $462.47
In Stock at Manufacturer

About Validated Cancer Antibodies

Every antibody here is validated for multiple cancer-research applications — two or more of Western blot, immunohistochemistry and immunofluorescence. Check each datasheet for the applications and images validated for your specific target. Use code ONCO15 for 15% off cancer antibodies.

How validated is “validated”? Choosing a cancer antibody

Two antibodies to the same target are not interchangeable. The deciding factor is the evidence behind the listing — in which applications, species, and with what images. This collection sets the bar at multi-application validation: every antibody is validated in two or more of WB, IHC and IF.

Decision dimension Multi-application validated (this collection) Single-application Catalog / RUO-only
Validation evidenceTwo or more of WB / IHC / IF, with datasheet dataOne application (often WB only)Specification sheet; little or no application data
Applications confirmed2+ (across lysate, tissue and/or cells)10–1, frequently predicted
Reproducibility riskLower — cross-application concordanceModerate — format-dependentHigher — unverified
Best forPublication-grade tumor work across methodsA single, well-defined assayEarly screening on a budget
Watch-outsConfirm the validated applications, species and clone for your modelMay not transfer to IHC/IFPlan to validate in-house first

How to read this: if your project spans lysate, tissue and cells — or is headed for publication — start here, where every antibody is validated in two or more of these applications. Confirm on the datasheet which applications are validated for your target. If you only need one assay, the broader Cancer Antibodies catalog has more single-application options; for non-cancer targets browse all Antibodies. Validation is per-clone and per-lot — always check the datasheet image for your specific application and species.

Recommended — best-selling validated cancer antibodies

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