| Field | Specification |
|---|---|
| Target | |
| Alternative names | Solute carrier family 2, facilitated glucose transporter member 1;Glucose transporter type 1, erythrocyte/brain;GLUT-1;HepG2 glucose transporter;SLC2A1;GLUT1; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human SLC2A1 recombinant protein (Position: R92-V492). Human SLC2A1 shares 98% and 98.3% amino acid (aa) sequence identity with mouse and rat SLC2A1, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes solute carrier family 2, facilitated glucose transporter member 1 (gene SLC2A1) in human, mouse and rat samples and is validated for flow cytometry, ICC/IF, IHC and Western blot. The predicted molecular weight is 54.1 kDa, and the supplier reports an observed band at about 55 kDa.
GLUT1 (SLC2A1) is a facilitative glucose transporter responsible for basal glucose uptake in many cells. It is highly expressed in red blood cells and in the endothelium of the blood–brain barrier, where it supplies the brain's main fuel, and its deficiency causes GLUT1 deficiency syndrome; it is also often upregulated in cancer cells.
| Target | Solute carrier family 2, facilitated glucose transporter member 1 (gene SLC2A1; UniProt P11166, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 54.1 kDa |
| Observed MW | 55 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse, Rat) |
| IHC (frozen sections) | 0.5–1 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 2 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): rat brain tissue, mouse brain tissue, mouse NIH3T3 cells, human placenta tissue, human A431 cells, rat PC-12 cells, mouse NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): mouse brain tissue, rat brain tissue, human placenta tissue, human mammary cancer tissue, human placenta cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Samples with confirmed staining (IHC): human placenta tissue.
Samples with confirmed staining (ICC/IF): HepG2 cells.
Samples with confirmed staining (ICC/IF): A549 cells.
Samples with a confirmed signal (flow cytometry): A431 cells.
Immunogen
Recombinant human SLC2A1 fragment (Arg92–Val492), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 98% and 98.3%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places solute carrier family 2, facilitated glucose transporter member 1 in the cell membrane, melanosome and photoreceptor inner segment. Tissue expression noted by UniProt (human): Detected in erythrocytes (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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Shan Xiuhong et al. (2013) Necrosis Degree Displayed in Computed Tomography Images Correlated With Hypoxia and Angiogenesis in Breast Cancer. Journal of Computer Assisted Tomography. 10.1097/RCT.0b013e318279abd1
Yan Shi et al. (2015) Caffeic Acid Phenethyl Ester inhibit Hepatic Fibrosis by Nitric Oxide Synthase and Cystathionine Gamma-Lyase in Rats. Medical Science Monitor. 10.12659/MSM.895272
Yanxiang Cheng et al. (2013) How does hypoxia inducible factor-1α participate in enhancing the glycolysis activity in cervical cancer?. Annals of Diagnostic Pathology. 10.1016/j.anndiagpath.2012.12.002
