Western blot target validation

2500Products
2500 products
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Western blot testing of IRS-1 antibody and Lane 1: HeLa; 2: A549; 3: 293T; 4: MCF-7 lysate, Expected size: 132~185KD depending on serine phosphorylation level
Target: IRS-1
Antibodies, Primary Antibodies Western Blot
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Western blot testing of human 1) Caco-2, 2) HepG2 and 3) A549 cell lysate with Rab8 antibody. Predicted molecular weight: ~24 kDa.
Target: Rab8
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
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Western blot testing of GLUR3 antibody and Lane 1: rat brain; 2: mouse brain lysate. Predicted/observed size ~99KD
Target: GLUR3
Antibodies, Primary Antibodies Western Blot
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Western blot testing of ST5 antibody and Lane 1: rat testis; 2: human A431; 3: (h) HeLa; 4: (h) COLO320; 5: mouse NIH3T3 lysate. Predicted/observed molecular weight: ~126kDa.
Target: ST5
Antibodies, Primary Antibodies Western Blot
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Western blot testing of CAT1 antibody and Lane 1: human placenta; 2: HeLa; 3: SKOV-3; 4: HT1080 cell lysate. Expected molecular weight ~68 kDa.
Target: CAT1
Antibodies, Primary Antibodies Western Blot
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Western blot testing of human 1) 293T and 2) HeLa cell lysate with Nicastrin antibody. Predicted molecular weight ~78 kDa, but can be observed at up to ~160 kDa due to glycosylation.
Target: Nicastrin
Antibodies, Primary Antibodies Western Blot
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Western blot testing of GPR30 antibody and Lane 1: human COLO320; 2: human MCF-7; 3: monkey COS7 cell lysate. Expected molecular weight: 40-60 kDa depending on glycosylation level.
Target: GPR30
Antibodies, Primary Antibodies Western Blot
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Western blot testing of MKK4 antibody and Lane 1: rat skeletal muscle; 2: HeLa; 3: A549; 4: MM231; 5: CEM lysate. Predicted molecular weight ~44 kDa.
Target: MKK4
Antibodies, Primary Antibodies Western Blot
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Western blot testing of 1) human PANC-1, 2) human HT1080, 3) human U-251, 4) rat C6 and 5) mouse testis tissue lysate with ATG5 antibody. Predicted molecular weight ATG5: ~32 kDa; ATG5/ATG12 heterodimer: ~56 kDa.
Target: ATG5
Antibodies, Primary Antibodies Western Blot
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Western blot testing of RIP3 antibody and Lane 1: PANC; 2: SW620; 3: SKOV-3; 4: M231 cell lysate
Target: RIP3
Antibodies, Primary Antibodies Western Blot
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Western blot testing of TLR1 antibody and Lane 1: COLO320; 2: SW620; 3: SKOV; 4: Jurkat; 5: CEM; 6: PANC lysate. Predicted molecular weight ~90 kDa.
Target: TLR1
Antibodies, Primary Antibodies Western Blot
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Western blot testing of Antithrombin III antibody and Lane 1: rat testis; 2: SMMC-7721; 3: Jurkat; 4: Raji cell lysate. Expected/observed size ~52KD
Target: III
Antibodies, Primary Antibodies Western Blot
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Western blot testing of TIA-1 antibody and Lane 1: Jurkat; 2: Raji; 3: CEM; 4: HT1080; 5: K562 cell lysate. Predicted molecular weight: ~43kDa and ~15kDa (granulocyte-associated isoform).
Target: TIA-1
Antibodies, Primary Antibodies Western Blot
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Western blot testing of PON1 antibody and Lane 1: rat liver; 2: (r) lung; 3: human placenta; 4: (r) testis; 5: (h) HeLa; 6: mouse HEPA; 7: (h) A549; 8: (h) Jurkat; 9: (h) SKOV lysate. Expected/observed molecular weight ~40 kDa.
Target: PON1
Antibodies, Primary Antibodies Western Blot
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Western blot testing of HNF1B antibody and mouse liver lysate. Predicted molecular weight ~61 kDa.
Target: HNF1B
Antibodies, Primary Antibodies Western Blot
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Western blot testing of PIASg antibody and Lane 1: human placenta; 2: A431 lysate. Expected/observed size ~56 kDa.
Target: PIAS4
Antibodies, Primary Antibodies Western Blot
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Western blot testing of KIN17 antibody and Lane 1: rat skeletal muscle; 2: human placenta; 3: rat testis tissue lysate. Predicted molecular weight ~45kDa.
Target: KIN17
Antibodies, Primary Antibodies Western Blot
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Western blot testing of Apoptosis-Inducing Factor antibody and Lane 1: rat heart; 2: (r) brain; 3: human K562; 4: (h) HEPG2; 5: (h) A431; 6: mouse NIH3T3 cell lysate. Expected/observed molecular weight: ~67kDa.
Target: AIFM1
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Immunofluorescence
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Western blot testing of 1) human HepG2, 2) human 293T, 3) rat kidney, 4) rat NRK and 5) mouse kidney tissue lysate with SGLT1 antibody. Expected molecular weight: ~73 kDa.
Target: SGLT1
Antibodies, Primary Antibodies Western Blot
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Western blot testing of RGS9 antibody and Lane 1: rat brain; 2: mouse brain tissue lysate. Predicted molecular weight: 50-77 kDa (multiple isoforms).
Target: RGS9
Antibodies, Primary Antibodies Western Blot
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Western blot testing of PROX1 antibody and Lane 1: rat thymus; 2: human HeLa; 3: (h) Jurkat; 4: (h) MM231 cell lysate. Predicted molecular weight is 83 kDa, observed at 80-110 kDa.
Target: PROX1
Antibodies, Primary Antibodies Western Blot
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Western blot testing of beta Arrestin 1 antibody and Lane 1: rat lung; 2: rat skeletal muscle; 3: human SW620 cell lysate. Predicted molecular weight ~51 kDa.
Target: ARRB1
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
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Western blot testing of FAT10 antibody and Lane 1: HeLa; 2: SKOV; 3: MCF-7; 4: A549; 5: SMMC-7721 cell lysate. Expected/observed size: 18-22 kDa.
Target: FAT10
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
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Western blot testing of human 1) HeLa, 2) A375 and 3) PANC cell lysate with COMT antibody. Predicted molecular weight: ~30/25kDa (isoforms 1/2).
Target: COMT
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
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Western blot testing of mouse kidney tissue lysate with SLC9A2 antibody. Expected molecular weight ~92 kDa.
Target: SLC9A2
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
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Western blot testing of Securin antibody and Lane 1: HeLa; 2: MCF-7; 3: SKOV; 4: A375 lysate. Predicted molecular weight ~22 kDa, can be observed at 25-30 kDa.
Target: Securin
Antibodies, Primary Antibodies Western Blot
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Western blot testing of PLTP antibody and 1. MCF7, 2. Raji and 3. HeLa lysate. Expected/observed size ~55KD
Target: PLTP
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
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Western blot testing of CDC6 antibody and Lane 1: Jurkat; 2: MCF-7 cell lysate. Predicted/observed molecular weight: ~62kDa
Target: CDC6
Antibodies, Primary Antibodies Western Blot
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IHC-P: FABP6 antibody testing of human lung cancer tissue
Target: FABP6
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
$449.00
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Western blot testing of Calponin antibody and human 1) HeLa, 2) Jurkat, and 3) MCF7 cell lysate. Expected molecular weight ~33 kDa.
Target: CNN1
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
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Western blot testing of HYAL1 antibody and Lane 1: rat NRK; 2: mouse liver; 3: (m) kidney; 4: (m) SP2/0 cell lysate
Target: HYAL1
Antibodies, Primary Antibodies Western Blot
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Western blot testing of SERPINA3 antibody and SMMC-7721 cell lysate. Predicted molecular weight: 47/65kDa (unmodified/glycosylated).
Target: SERPINA3
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
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Western blot testing of SUMO1 antibody and Lane 1: rat spleen; 2: human placenta tissue lysate. Predicted molecular weight: 12-15 kDa
Target: SUMO1
Antibodies, Primary Antibodies Western Blot
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Western blot testing of CCR3 antibody and Lane 1: K562; 2: Raji cell lysate. Expected molecular weight: 40~55 kDa.
Target: CCR3
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
$449.00
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Western blot testing of IRF5 antibody and Lane 1: human placenta; 2: rat thymus; 3: (r) kidney; 4: (r) ovary tissue lysate. Expected/observed molecular weight ~57kDa.
Target: IRF5
Antibodies, Primary Antibodies Western Blot
$449.00
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Immunofluorescent staining of FFPE human U-2 OS cells with IRF8 antibody (green) and Alpha Tubulin mAb (red). HIER: steam section in pH6 citrate buffer for 20 min.
Target: IRF8
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Immunofluorescence
$449.00
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