Western blot target validation

2500Products
2500 products
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Western blot testing of Alpha 2a Adrenergic Receptor antibody and Lane 1: human HeLa; 2: human PANC-1 cell lysate. Predicted molecular weight ~49 kDa.
Alpha 2a Adrenergic Receptor Antibody
Target: ADRA2A
Antibodies, Primary Antibodies Western Blot
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Western blot testing of Integrin alpha 7 antibody and Lane 1: 293T; 2: A431; 3: HeLa; 4: Jurkat; 5: Raji cell lysate. Expected/observed size ~129KD
Integrin alpha 7 Antibody (ITGA7)
Target: ITGA7
Antibodies, Primary Antibodies Western Blot
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Western blot testing of 1) human HepG2, 2) human HUH7, 3) rat liver, 4) rat RH35 and 5) mouse liver tissue lysate with HNF6 antibody. Predicted molecular weight ~51 kDa.
HNF6 Antibody / Hepatocyte nuclear factor 6 / ONCECUT1
Target: HNF6
Antibodies, Primary Antibodies Western Blot
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Western blot testing of IRAKM antibody and Lane 1: HeLa; 2: Jurkat; 3: HUT102 cell lysate. Predicted molecular weight: ~68kDa.
IRAKM Antibody
Target: IRAKM
Antibodies, Primary Antibodies Western Blot
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Western blot testing of Olig1 antibody and Lane 1: U87; 2: HeLa lysate
Olig1 Antibody
Target: Olig1
Antibodies, Primary Antibodies Western Blot
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Western blot testing of SBP antibody and Lane 1: COLO320; 2: PANC cell lysate
SBP Antibody / Selenium Binding Protein / SELENBP1
Target: SBP
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
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Western blot testing of KLF4 antibody and Lane 1: HeLa; 2: A549; 3: U20S cell lysate. Predicted molecular weight: 50-60 kDa + possible ~75 kDa (phosphorylated form).
KLF4 Antibody
Target: KLF4
Antibodies, Primary Antibodies Western Blot
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Western blot testing of 1) rat brain and 2) mouse brain lysate. Predicted molecular weight ~53 kDa.
Dopamine Receptor D5 Antibody
Target: DRD5
Antibodies, Primary Antibodies Western Blot
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Western blot testing of MCT5 antibody and lysate from 1: rat testis; 2: human Jurkat; 3: (h) HeLa; 4: (h) MCF-7 cells. Predicted molecular weight: ~54 kDa.
MCT5 Antibody / SLC16A4
Target: MCT5
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
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Western blot testing of Leupaxin antibody and Lane 1: rat thymus; 2: human Jurkat cell lysate. Expected/observed size ~43KD
Leupaxin Antibody
Target: LPXN
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
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Western blot testing of SLC1A4 antibody and Lane 1: human U87; 2: rat brain; 3: mouse brain tissue lysate. Expected/observed size 60~80KD
SLC1A4 Antibody
Target: SLC1A4
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
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Western blot testing of MMP12 antibody and Lane 1: human SMMC-7721; 2: mouse HEPA; 3: (h) HeLa; 4: (h) K562; 5: (h) MCF-7 cell lysate. Predicted molecular weight: ~55 kDa (pro form), ~45 kDa and ~22 kDa (active forms).
MMP12 Antibody
Target: MMP12
Antibodies, Primary Antibodies Western Blot
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Western blot testing of LAMA2 antibody and Lane 1: HeLa; 2: A549; 3: PANC lysate. Predicted molecular weight ~344 kDa.
LAMA2 Antibody / Laminin alpha 2
Target: LAMA2
Antibodies, Primary Antibodies Western Blot
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Immunofluorescent staining of FFPE human U-2 OS cells with EGR2 antibody (green) and Alpha Tubulin mAb (red). HIER: steam section in pH6 citrate buffer for 20 min.
EGR2 Antibody / Early growth response 2
Target: EGR2
Antibodies, Primary Antibodies Western Blot, Immunofluorescence
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Western blot testing of FLI1 antibody and Lane 1: Jurkat; 2: Raji cell lysate. Predicted/observed molecular weight: ~51kDa.
FLI1 Antibody
Target: FLI1
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
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Western blot testing of OCT1 antibody and Lane 1: human HeLa cell lysate; 2: human A549 cell lysate. Predicted molecular weight ~61 kDa but this glycoprotein may be observed at up to ~80 kDa.
OCT1 Antibody / SLC22A1
Target: OCT1
Antibodies, Primary Antibodies Western Blot
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Western blot testing of MMP24 antibody and Lane 1: PANC; 2: HeLa; 3: SMMC-7721; 4: A549 cell lysate
MMP24 Antibody
Target: MMP24
Antibodies, Primary Antibodies Western Blot
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Western blot testing of Profilin 2 antibody and rat brain lysate. Expected/observed size ~15 kDa.
Profilin 2 Antibody / PFN2
Target: PFN2
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Flow Cytometry
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Western blot testing of GJC1 antibody and MCF-7 cell lysate. Expected/observed molecular weight ~45 kDa.
GJC1 Antibody / Connexin 45
Target: GJC1
Antibodies, Primary Antibodies Western Blot
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Western blot testing of EAAT3 antibody and human placenta tissue lysate. Expected molecular weight: 57-70 kDa.
EAAT3 Antibody / SLC1A1
Target: EAAT3
Antibodies, Primary Antibodies Western Blot
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Immunofluorescent staining of FFPE human colon cancer tissue with NKCC1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
NKCC1 Antibody / SLC12A2
Target: NKCC1
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Immunofluorescence
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Flow cytometry testing of fixed and permeabilized human HepG2 cells with IGF2R antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= IGF2R antibody.
IGF2R Antibody / M6PR
Target: IGF2R
Antibodies, Primary Antibodies Western Blot, Flow Cytometry
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Western blot testing of Angiotensin Converting Enzyme antibody and Lane 1: A549; 2: HeLa; 3: 22RV1 lysate. Expected molecular weight 140-170 kDa.
Angiotensin Converting Enzyme Antibody (ACE)
Target: ACE
Antibodies, Primary Antibodies Western Blot
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Western blot testing of human 1) HepG2, 2) 293T and 3) Raji cell lysate with EIF2B antibody. Expected molecular weight: ~38 kDa.
EIF2B Antibody / EIF2S2
Target: EIF2B
Antibodies, Primary Antibodies Western Blot
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Western blot testing of Cdk6 antibody and Lane 1: rat testis; 2: (r) lung tissue lysate. Predicted molecular weight: 36-40 kDa.
Cdk6 Antibody
Target: Cdk6
Antibodies, Primary Antibodies Western Blot
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Western blot testing of IRAK1 antibody and Lane 1: rat liver; 2: human placenta; 3: MCF-7; 4: PANC cell lysate. Predicted/observed molecular weight: ~76 kDa.
IRAK1 Antibody
Target: IRAK1
Antibodies, Primary Antibodies Western Blot
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IHC-P: ACE antibody testing of human prostate cancer tissue
ACE Antibody [Angiotensin Converting Enzyme]
Target: ACE
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
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Western blot testing of 1) human HepG2, 2) human PC-3, 3) monkey COS-7, 4) rat liver, 5) mouse liver and 6) mouse kidney tissue lysate with HYAL1 antibody. Expected molecular weight: 48-60 kDa.
HYAL1 Antibody
Target: HYAL1
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Immunofluorescence
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Western blot testing of OCTN2 antibody and Lane 1: rat kidney; 2: (r) skeletal muscles; 3: mouse liver; 4: (m) HEPA; 5: (m) NIH3T3 cell lysate. Expected/observed size ~63KD
OCTN2 Antibody / SLC22A5
Target: OCTN2
Antibodies, Primary Antibodies Western Blot
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IHC-P: MMP14 antibody testing of human intestinal cancer tissue
MMP14 Antibody
Target: MMP14
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
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IHC staining of FFPE mouse kidney tissue with NKCC2 antibody, HRP-secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
NKCC2 Antibody / SLC12A1
Target: NKCC2
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
$449.00
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Western blot testing of human 1) HeLa and 2) COLO320 cell lysate with HDAC5 antibody. Predicted molecular weight ~122 kDa, observed at 122-160 kDa.
HDAC5 Antibody
Target: HDAC5
Antibodies, Primary Antibodies Western Blot
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Western blot testing of SMAD5 antibody and Lane 1: human K562; 2: (h) Jurkat; 3: rat PC-12; 4: (h) HeLa; 5: (h) SMMC-7721 cell lysate. Observed molecular weight: 52~60 kDa.
SMAD5 Antibody
Target: SMAD5
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
$449.00
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IHC staining of FFPE human colorectal adenocarcinoma tissue with SLC12A6 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
SLC12A6 Antibody
Target: SLC12A6
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
$449.00
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Western blot testing of TBK1 antibody and Lane 1: HeLa; 2: rat testis; 3: rat liver; Predicted molecular weight: ~84 kDa.
TBK1 Antibody
Target: TBK1
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
$449.00
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Western blot testing of E2F4 antibody and Lane 1: rat lung; 2: human SMMC-7721 lysate. Expected molecular weight ~44 kDa (unmodified) and 60-65 kDa (phosphorylated).
E2F4 Antibody
Target: E2F4
Antibodies, Primary Antibodies Western Blot
$449.00
$449.00
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