Western blot target validation

2500Products
2500 products
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IHC staining of FFPE human liver cancer tissue with PCSK9 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Target: PCSK9
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
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Western blot testing of CCR9 antibody and Lane 1: Jurkat; 2: HeLa; 3: SMMC-7721 cell lysate. Expected/observed size ~43KD
Target: CCR9
Antibodies, Primary Antibodies Western Blot
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Western blot testing of Cdc37 antibody and 1. Jurkat, 2. 293T cell lysate. Expected/observed molecular weight 44~50kDa.
Target: Cdc37
Antibodies, Primary Antibodies Western Blot
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Western blot testing of P2X7 antibody and human U87-MG cell lysate. Expected molecular weight: 65~85 kDa depending on glycosylation level.
Target: P2X7
Antibodies, Primary Antibodies Western Blot
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Western blot testing of BAG2 antibody; Lane 1: rat testis; 2: HeLa; 3: A549; 4: A431 cell lysate. Predicted/observed molecular weight: ~22kDa.
Target: BAG2
Antibodies, Primary Antibodies Western Blot
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Western blot testing of human 1) SK-OV-3, 2) U-2 0S, 3) HeLa and 4) SMMC-7721 cell lysate. Expected molecular weight ~66 kDa (unmodified), 85-95 kDa (glycosylated).
Target: PTH1R
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Immunofluorescence
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Western blot testing of SIDT1 antibody and Lane 1: HeLa; 2: COLO320; 3: SW620; 4: HT1080 cell lysate
Target: SIDT1
Antibodies, Primary Antibodies Western Blot
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Western blot testing of Caspase-12 antibody; Lane 1: PANC; 2: SMMC-7721; 3: A549; 4: HeLa. Predicted molecular weight ~50/36~42kDa (pro/active).
Target: CASP12
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
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Western blot testing of Aquaporin 0 antibody and Lane 1: mouse spleen; 2: mouse intestine; Observed size: 28-60KD, depending on level of glycosylation
Target: AQP0
Antibodies, Primary Antibodies Western Blot
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Western blot testing human 1) U-251, 2) SH-SY5Y, 3) U-87 MG and 4) U-2 OS cell lysate. Predicted molecular weight: ~105/125 kDa (unmodified/glycosylated).
Target: ADAM19
Antibodies, Primary Antibodies Western Blot
$449.00
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Western blot testing of RUNX3 antibody and 1. rat liver and 2. mouse liver. Predicted molecular weight: ~44 kDa, isoforms can be observed at 42-48 kDa.
Target: RUNX3
Antibodies, Primary Antibodies Western Blot
$449.00
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Western blot testing of RGS3 antibody and Lane 1: rat testis; 2: (r) brain; 3: (r) spleen; 4: human HeLa; 5: (h) U87 cell lysate
Target: RGS3
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
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Western blot testing of Proprotein Convertase 1/3 antibody; Lane 1: rat liver; 2: (r) thymus; and human samples 3: A549; 4: HeLa; 5: COLO320; 6: PANC cell lysate
Target: PCSK1
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Immunofluorescence
$449.00
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Western blot testing of Cystatin A antibody and human A431 cell lysate. Predicted molecular weight ~11 kDa.
Target: CSTA
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
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Western blot testing of GCN2 antbody; Lane 1: Recombinant mouse protein 10ng; 2: 5ng; 3: 2.5ng; 4: 1.25ng
Target: GCN2
Antibodies, Primary Antibodies Western Blot
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Western blot testing of SMAD1 antibody and Lane 1: SMMC-7721; 2: K562; 3: HT1080; 4: HeLa; 5: Jurkat cell lysate. Predicted molecular weight: 52~60 kDa.
Target: SMAD1
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Immunofluorescence
$449.00
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Western blot testing of ALDH2 antibody and rat samples; Lane 1: liver; 2: intestine; 3: lung; 4: kidney tissue lysate. Predicted molecular weight: ~56kDa.
Target: ALDH2
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
$449.00
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IHC staining of FFPE human breast cancer tissue with Ataxin-2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Target: Ataxin-2
Antibodies, Primary Antibodies Western Blot, Flow Cytometry, Immunofluorescence, Immunohistochemistry
$449.00
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Western blot testing of CXCR4 antibody; Lane 1: M231; 2: MCF-7; 3: Jurkat cell lysate. Predicted molecular weight ~40 kDa but may be observed at higher molecular weights due to glycosylation.
Target: CXCR4
Antibodies, Primary Antibodies Western Blot
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Western blot testing of VE Cadherin antibody; Lane 1: A549; 2: HeLa; 3: MCF-7 cell lysate. Expected molecular weight: 90~140 kDa depending on glycosylation level.
Target: CDH5
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
$449.00
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Western blot testing of Aquaporin 6 antibody and Lane 1: U87; 2: COLO320; 3: MCF-7 cell lysate
Target: AQP6
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
$449.00
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Western blot testing of SPARCL1 antibody and Lane 1: rat lung; 2: mouse lung; and human samples 3: PANC; 4: A549; 5: COLO320; 6: MCF-7; 7: HT1080 cell lysate. Predicted/observed molecular weight ~75 kDa.
Target: SPARCL1
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
$449.00
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Western blot testing of human HepG2 cell lysate with FMO5 antibody. Expected molecular weight ~60 kDa.
Target: FMO5
Antibodies, Primary Antibodies Western Blot
$449.00
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Flow cytometry testing of human PBM cells with CXCR1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CXCR1 antibody.
Target: CXCR1
Antibodies, Primary Antibodies Western Blot, Flow Cytometry
$449.00
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Western blot testing of APLP1 antibody and 1) human HeLa, 2) rat PC-12, and 3) mouse HEPA1-6 lysate; Expected molecular weight: 76 kDa (unmodified), ~86 kDa (soluble/glycosylated form), 92~95 kDa (mature form).
Target: APLP1
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
$449.00
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Western blot testing of Proprotein Convertase 4 antibody and rat brain tissue. Observed size 54~84KD depending on glycosylation level
Target: PCSK4
Antibodies, Primary Antibodies Western Blot
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Immunofluorescent staining of FFPE human A549 cells with PMP70 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Target: PMP70
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Immunofluorescence
$449.00
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Western blot testing of rat 1) heart, 2) kidney, 3) lung and human 4) A549, 5) A431 and 6) COLO320 lysate with WISP1 antibody. Expected molecular weight: 40-55 kDa depending on degree of glycosylation.
Target: WISP1
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry, Immunofluorescence
$449.00
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Western blot testing of EIF2S1 antbody; Lane 1: COLO320; 2: CEM; 3: Raji; 4: HT1080 cell lysate. Predicted molecular weight: 36 kDa.
Target: EIF2S1
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
$449.00
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Western blot testing of CXCR6 antibody and Lane 1: HeLa; 2: Jurkat; 3: MCF-7 lysate. Expected/observed molecular weight: ~39 kDa.
Target: CXCR6
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
$449.00
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IHC staining of FFPE human rectal cancer tissue with ALDH3A1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Target: ALDH3A1
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
$449.00
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Western blot testing of ADAM2 antibody in human SMMC-7721 cell lysate. Predicted molecular weight ~82 kDa, but can be observed at ~100 kDa.
Target: ADAM2
Antibodies, Primary Antibodies Western Blot
$449.00
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Western blot testing of Prolactin Receptor antibody and Lane 1: human HeLa; 2: human MCF-7 cell lysate. Expected molecular weight: 70-117 kDa depending on glycosylation level.
Target: PRLR
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
$449.00
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Western blot testing of PGK1 antbody; Lane 1: rat liver; 2: (r) brain; 3: (r) lung; and human samples 4: A431; 5: COLO320; 6: HeLa; 7: A549; 8: Jurkat cell lysate. Expected/observed molecular weight ~44kDa.
Target: PGK1
Antibodies, Primary Antibodies Western Blot, Immunohistochemistry
$449.00
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Western blot testing of ABCB4 antibody and human HepG2 cell lysate. Predicted molecular weight ~142 kDa.
Target: ABCB4
Antibodies, Primary Antibodies Western Blot
$449.00
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Western blot testing of Prothrombin antbody and rat testis lysate; Predicted molecular weight: 70 kDa, but may be observed at higher molecular weights due to glycosylation.
Target: F2
Antibodies, Primary Antibodies Western Blot
$449.00
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