| Field | Specification |
|---|---|
| Species |
DU145 is a human prostate cancer cell with an epithelial morphology commonly used in prostate cancer research. The cell line was established from the brain of a 69-year-old male with prostate cancer. They express androgen receptors and are considered tumorigenic with moderate metastatic potential, forming adenocarcinoma (grade II) consistent with prostatic primary when injected into nude mice.
In terms of karyotype, DU145 cells are hypotriploid and have several marker chromosomes, including t(11q12q), del(11)(q23), 16q+, del(9)(p11), del(1)(p32), among others. They express several isoenzymes, including AK-1, ES-D, G6PD, GLO-I, Me-2, PGM1, and PGM3. However, the cells do not express the prostate antigen.
DU145 cells are weakly positive for acid phosphatase and capable of forming colonies in soft agar. The presence of microvilli, tonofilaments, desmosomes, any mitochondria, well-developed Golgi, and heterogenous lysosomes was reported by ultrastructural analyses. DU145 cells have a doubling time of approximately 30-40 hours and are suitable transfection hosts.
DU145 cells are a valuable tool in the therapeutic research of prostate cancer. Along with PC3 and LNCaP cell lines, DU145 is a standard prostate cancer cell line used in medicinal research. Along with PC-3 cells, DU-145 cells express androgen receptor proteins. However, when treated with an androgen ligand, the cells did not show stimulation of the activity of an AR-responsive reporter gene. Therefore, these cells are considered to be androgen non-responsive.
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- Antigen expression: Blood Type O, Rh+
- Isoenzymes: Me-2, 1-2, PGM3, 2, PGM1, 1, ES-D, 1, AK-1, 1, G6PD, B, GLO-1, 2, Phenotype Frequency Product: 0.0041
- Tumorigenic: Forms adenocarcinoma (grade II) consistent with prostatic primary
- Karyotype: (P75) hypotriploid to tetraploid with abnormalities including breaks, dicentrics, minutes and large telocentric marker
- cultureMedium: EMEM (MEM Eagle), w: 2 mM L-Glutamine, w: 2.2 g/L NaHCO3, w: EBSS (Cytion article number 820100a)
- supplements: Supplement the medium with 10% FBS and 1% NEAA
- dissociationReagent: Accutase
- subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
- seedingDensity: 2 x 104 cells/cm2 will yield in a confluent layer in about 4 days
- fluidRenewal: 2 to 3 times per week
- postThawRecovery: After thawing, allow the cells to recover from the freezing process for at least 24 hours.
- freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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