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2× Hieff™ Ultra-Rapid II HotStart Universal PCR Master Mix contains antibody-modified heat-stabilized Taq DNA Polymerase with a strong elongation factor and optimized buffer system for ultra-high amplification efficiency. Particularly suited for direct colony PCR from E. coli, Agrobacterium, and yeast. Pre-loaded red tracer dye enables direct gel electrophoresis. Validated shelf life of 2 years at −20°C.
Features
- Antibody-based Hot Start: prevents non-specific amplification during setup
- Ultra-rapid amplification: 1 sec/kb for simple templates ≤6 kb
- Colony PCR compatible: E. coli, Agrobacterium, and yeast
- Pre-loaded red tracer dye for direct gel loading
- Long-fragment capability up to >10 kb from genomic DNA
- 2-year stability at −25 to −15°C
Extension Speed Guide
| Template Type | Fragment Size | Extension Rate |
|---|---|---|
| Complex templates | ≤3 kb | 1 sec/kb |
| Complex templates | 3–6 kb | 3 sec/kb |
| Complex templates | 6–10 kb | 5 sec/kb |
| Complex templates | >10 kb | 10 sec/kb |
| Simple templates (plasmid) | ≤6 kb | 1 sec/kb |
Selected Citations
[1] Rapid, sensitive, and visual detection of mandarin fish ranavirus using RPA-CRISPR/Cas12a. Front. Microbiol. 2024.
[2] CRISPR/Cas9-mediated resistance to wheat dwarf virus in hexaploid wheat. Viruses. 2024.
This ultra-rapid mix achieves 1 sec/kb for simple templates such as plasmids up to 6 kb. For complex templates, extension rates are 1 sec/kb for ≤3 kb, 3 sec/kb for 3–6 kb, 5 sec/kb for 6–10 kb, and 10 sec/kb for >10 kb fragments from genomic DNA.
The heat-stabilized Taq DNA Polymerase is modified with monoclonal antibodies that block polymerase activity at ambient temperature. At initial denaturation (≥95°C), the antibodies dissociate irreversibly, releasing fully active enzyme. This prevents non-specific priming during reaction assembly.
Yes. This mix is specifically validated for direct colony PCR from E. coli, Agrobacterium, and yeast. Colony material can be added directly to the reaction without prior culture preparation or DNA extraction.
No. The mix contains a pre-loaded red tracer dye enabling direct gel loading of PCR products immediately after amplification.
The formulation contains a protective agent validated for 2 years of stability at −25 to −15°C. High stability is confirmed by the manufacturer for the full stated shelf life.
Yeasen Biotechnology supports custom formulation and scale-up services for reagent mixes and master mixes. Contact BioHippo to discuss project requirements and initiate an inquiry.
▶ GMP-Grade & Bulk Supply
Select Yeasen reagent mixes are available in GMP grade for IVD assay development. Manufactured in an ISO 13485-certified UCF.ME™ facility with FDA Drug Master File (DMF) support.
- GMP release testing and CoA documentation
- Scalable from milliliter to liter-scale quantities
- Consistent lot-to-lot performance specifications
▶ Glycerol-Free & Lyophilization-Ready Formats
Glycerol-free and lyophilization-ready formats are available for reagents intended for cartridge-based point-of-care devices, liquid handling automation, or ambient-temperature distribution.
- Glycerol-free liquid formulation
- Lyophilization-compatible format with cryoprotectant
- Custom reaction buffer optimization
▶ Molecular IVD RDC Service
Yeasen's Research and Development Contracting team provides end-to-end support for molecular diagnostic product development using Yeasen reagent mixes as a foundation.
- Master mix selection and performance matching for target assay
- Primer/probe design and assay buffer optimization
- Sensitivity, specificity, and LOD validation
- Instrument compatibility assessment
▶ Custom Formulation
Custom formulation services are available for researchers requiring modifications to standard reagent mix compositions, including ROX level adjustment, dye substitution, stabilizer optimization, and buffer modification.
- ROX concentration customization (No / Low / High ROX)
- Reference dye and indicator dye options
- Buffer composition and pH optimization
- Scale-up to commercial quantities on confirmation
ⓘ Customization services fulfilled by Yeasen Biotechnology. Contact BioHippo for project scoping and pricing.
