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Scientific Background
Middle East Respiratory Syndrome Coronavirus 3C-like protease (MERS-CoV 3CL Protease), GenBank Accession No. ATG84821.1, a.a. 1-306(full length), with an N-terminal MBP-tag, and C-terminal His-tag, expressed in an E. coli expression system, MW=78 kDa.
Product Description
E. coli ExpressionRecombinant 3CL Protease MERS-CoV protein is produced using a validated E. coli expression system and supplied in aqueous buffer solution. Suitable for enzyme kinetics, inhibitor screening, binding assays, structural studies, and related biochemical research applications.
Protein Specifications
| UniProt ID | A0A291I686 |
|---|---|
| Expression System | E. coli |
| Amino Acids / Region | 1-306(full length) |
| Affinity Tag | N-terminal MBP-tag, C-terminal His-tag |
| Molecular Weight | 78 kDa |
| Formulation | 40 mM Tris-HCl, pH 8.0, 138 mM NaCl, 2.7 mM KCl, 20% glycerol, variable Imidazol… |
| Storage | Aqueous buffer solution. |
| Biosafety Level | Not applicable (BSL-1) |
Safety & Handling
Avoid freeze/thaw cycles.
This protein was produced in E. coli. Expression system selection determines post-translational processing, disulfide bond formation, and co-factor incorporation — all of which affect enzymatic activity. Insect cell (Sf9) systems are preferred for kinases and multi-subunit enzymes that require phosphorylation or chaperone assistance; E. coli is used for structurally simpler proteins.
This protein spans amino acids 1-306(full length). Confirm the region includes your domain of interest — the active site, binding pocket, or substrate recognition sequence — before placing your order. Refer to the UniProt database for domain annotation.
Purity is assessed by SDS-PAGE; see the Certificate of Analysis. A gel image is provided with each lot. BPS Bioscience performs rigorous QC on each lot, including purity assessment and functional activity testing where applicable. Contact technical support if purity ≥99% is required for biophysical measurements.
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Refer to the product datasheet for validated protocols and recommended assay conditions. Contact BioHippo technical support for application-specific guidance.
Aqueous buffer solution. Avoid repeated freeze-thaw cycles — prepare single-use working aliquots. Add BSA or glycerol to aliquots if storing diluted enzyme is necessary. Typical stability is at least 6 months at −80°C.
BioHippo offers flexible sourcing for qualified research institutions and partners.
- Bulk quantities: Large-scale orders for HTS campaigns or structural studies.
- Custom constructs: Alternative tag positions, truncation variants, or point mutants may be available upon request.
- Biotinylated variants: Avi-Tag site-specific biotinylation is available for SPR/BLI surface capture applications.
- Extended QC data: Activity assay data, SEC-HPLC profiles, or additional purity methods available on request.
Contact BioHippo customer support for custom requirements.
- Jo, S. et al., J. Enzyme Inh. & Med. Chem. 2019; 1(35): 145-151.
- He, J. et al., Intl J. Antimicrobial Agents. 2020; doi.org/10.1016/j.ijantimicag.2020.106055.
