5,7-Dichlorokynurenic acid

Product details

• Chemical name: 5,7-dichloro-4-oxo-1H-quinoline-2-carboxylic acid.
• Also known as: 5,7-DCKA
• CAS number: 131123-76-7
• Molecular formula: C10H5Cl2NO3.
• Purity: >98%
• Concentration: 0.5-20 µM.
• Form: Lyophilized powder.
• Solubility: 200 mM in DMSO. Centrifuge all product preparations before use (10 000 X g for 1 min).
• Reconstitution: 200 mM in DMSO. Centrifuge all product preparations before use (10 000 X g for 1 min).
• Shipping: Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
• Target: NMDA receptors
• Source: Synthetic
• Activity: 5,7-Dichlorokynurenic acid is an NMDA receptor antagonist acting at the glycine site1.
• Alternative names: 5,7-DCKA

Scientific background

5,7-Dichlorokynurenic acid (5,7-DCKA), is a derivative of kynurenic acid, an extremely potent excitatory amino acid receptor antagonist of N-methyl-D-aspartate (NMDA) receptors. 5,7-DCKA binds with high affinity to a strychnine-insensitive glycine binding site located on the NMDA receptor. This site is considered to be used as a therapeutic target for acute focal ischemia. The compound exhibits strong selectivity for binding to GluN1 subunit over GluN3A1,2 with an IC50 value of 79 nM in rat cortical and hippocampal membranes4.Small concentrations of 5,7-DCKA have the ability to inhibit several processes including NMDA-stimulated elevation of cytosolic calcium in cultured hippocampal neurons, cGMP accumulation in cerebellar slices, and norepinephrine release from hippocampal regions1.Several different studies have demonstrated potent anticonvulsant features of 5,7-DCKA when the compound is administrated intra-cerebroventricularly directly to the brain1.NMDA receptors play an important role in a variety of cellular processes and brain functions such as synaptic plasticity, addiction and stroke. They mediate several physiological functions including learning and memory formation; they play a role in glutamate excitotoxicity and are involved in many neurodegenerative conditions including Alzheimer's disease.

Lead time: 1-2 Business Days

Country of origin: Israel/IL

Applications key

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

Bioassay tested: Yes

• SDS (PDF)

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